Wildtype heterogeneity contributes to clonal variability in genome edited cells

• Published in: Scientific reports Vol. 12; no. 1; p. 18211
• Main Authors: Westermann, Lukas, Li, Yong, Göcmen, Burulca, Niedermoser, Matthias, Rhein, Kilian, Jahn, Johannes, Cascante, Isabel, Schöler, Felix, Moser, Niklas, Neubauer, Björn, Hofherr, Alexis, Behrens, Yvonne Lisa, Göhring, Gudrun, Köttgen, Anna, Köttgen, Michael, Busch, Tilman
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.10.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/80; 692/4022; Cell Line; Cell lines; Cells, Cultured; Cloning; CRISPR; CRISPR-Cas Systems - genetics; Gene Editing - methods; Genetic variability; Genome editing; Genomes; Genotypes; Heterogeneity; Humanities and Social Sciences; multidisciplinary; Phenotypes; Reproducibility; Reproducibility of Results; Science; Science (multidisciplinary)

Genome editing tools such as CRISPR/Cas9 enable the rapid and precise manipulation of genomes. CRISPR-based genome editing has greatly simplified the study of gene function in cell lines, but its widespread use has also highlighted challenges of reproducibility. Phenotypic variability among differen...