A quantitative reverse transcription-polymerase chain reaction for detection of Getah virus
Getah virus (GETV), a mosquito-borne alphavirus, is an emerging animal pathogen causing outbreaks among racehorses and pigs. Early detection of the GETV infection is essential for timely implementation of disease prevention and control interventions. Thus, a rapid and accurate nucleic acid detection...
Saved in:
| Published in | Scientific reports Vol. 8; no. 1; pp. 17632 - 7 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
London
Nature Publishing Group UK
05.12.2018
Nature Publishing Group |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Getah virus (GETV), a mosquito-borne alphavirus, is an emerging animal pathogen causing outbreaks among racehorses and pigs. Early detection of the GETV infection is essential for timely implementation of disease prevention and control interventions. Thus, a rapid and accurate nucleic acid detection method for GETV is highly needed. Here, two TaqMan minor groove binding (MGB) probe-based quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were developed. The qRT-PCR primers and TaqMan MGB probe were designed based on the conserved region of nsP1 and nsP2 genes of 23 GETV genome sequences retrieved from GenBank. Only the qRT-PCR assay using nsP2-specific primers and probe detected all two Malaysia GETV strains (MM2021 and B254) without cross-reacting with other closely related arboviruses. The qRT-PCR assay detected as few as 10 copies of GETV RNA, but its detection limit at the 95% probability level was 63.25 GETV genome copies (probit analysis,
P
≤ 0.05). Further validation of the qRT-PCR assay using 16 spiked simulated clinical specimens showed 100% for both sensitivity and specificity. In conclusion, the qRT-PCR assay developed in this study is useful for rapid, sensitive and specific detection and quantification of GETV. |
|---|---|
| AbstractList | Getah virus (GETV), a mosquito-borne alphavirus, is an emerging animal pathogen causing outbreaks among racehorses and pigs. Early detection of the GETV infection is essential for timely implementation of disease prevention and control interventions. Thus, a rapid and accurate nucleic acid detection method for GETV is highly needed. Here, two TaqMan minor groove binding (MGB) probe-based quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were developed. The qRT-PCR primers and TaqMan MGB probe were designed based on the conserved region of nsP1 and nsP2 genes of 23 GETV genome sequences retrieved from GenBank. Only the qRT-PCR assay using nsP2-specific primers and probe detected all two Malaysia GETV strains (MM2021 and B254) without cross-reacting with other closely related arboviruses. The qRT-PCR assay detected as few as 10 copies of GETV RNA, but its detection limit at the 95% probability level was 63.25 GETV genome copies (probit analysis, P ≤ 0.05). Further validation of the qRT-PCR assay using 16 spiked simulated clinical specimens showed 100% for both sensitivity and specificity. In conclusion, the qRT-PCR assay developed in this study is useful for rapid, sensitive and specific detection and quantification of GETV. Getah virus (GETV), a mosquito-borne alphavirus, is an emerging animal pathogen causing outbreaks among racehorses and pigs. Early detection of the GETV infection is essential for timely implementation of disease prevention and control interventions. Thus, a rapid and accurate nucleic acid detection method for GETV is highly needed. Here, two TaqMan minor groove binding (MGB) probe-based quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were developed. The qRT-PCR primers and TaqMan MGB probe were designed based on the conserved region of nsP1 and nsP2 genes of 23 GETV genome sequences retrieved from GenBank. Only the qRT-PCR assay using nsP2-specific primers and probe detected all two Malaysia GETV strains (MM2021 and B254) without cross-reacting with other closely related arboviruses. The qRT-PCR assay detected as few as 10 copies of GETV RNA, but its detection limit at the 95% probability level was 63.25 GETV genome copies (probit analysis, P ≤ 0.05). Further validation of the qRT-PCR assay using 16 spiked simulated clinical specimens showed 100% for both sensitivity and specificity. In conclusion, the qRT-PCR assay developed in this study is useful for rapid, sensitive and specific detection and quantification of GETV.Getah virus (GETV), a mosquito-borne alphavirus, is an emerging animal pathogen causing outbreaks among racehorses and pigs. Early detection of the GETV infection is essential for timely implementation of disease prevention and control interventions. Thus, a rapid and accurate nucleic acid detection method for GETV is highly needed. Here, two TaqMan minor groove binding (MGB) probe-based quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were developed. The qRT-PCR primers and TaqMan MGB probe were designed based on the conserved region of nsP1 and nsP2 genes of 23 GETV genome sequences retrieved from GenBank. Only the qRT-PCR assay using nsP2-specific primers and probe detected all two Malaysia GETV strains (MM2021 and B254) without cross-reacting with other closely related arboviruses. The qRT-PCR assay detected as few as 10 copies of GETV RNA, but its detection limit at the 95% probability level was 63.25 GETV genome copies (probit analysis, P ≤ 0.05). Further validation of the qRT-PCR assay using 16 spiked simulated clinical specimens showed 100% for both sensitivity and specificity. In conclusion, the qRT-PCR assay developed in this study is useful for rapid, sensitive and specific detection and quantification of GETV. Getah virus (GETV), a mosquito-borne alphavirus, is an emerging animal pathogen causing outbreaks among racehorses and pigs. Early detection of the GETV infection is essential for timely implementation of disease prevention and control interventions. Thus, a rapid and accurate nucleic acid detection method for GETV is highly needed. Here, two TaqMan minor groove binding (MGB) probe-based quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were developed. The qRT-PCR primers and TaqMan MGB probe were designed based on the conserved region of nsP1 and nsP2 genes of 23 GETV genome sequences retrieved from GenBank. Only the qRT-PCR assay using nsP2-specific primers and probe detected all two Malaysia GETV strains (MM2021 and B254) without cross-reacting with other closely related arboviruses. The qRT-PCR assay detected as few as 10 copies of GETV RNA, but its detection limit at the 95% probability level was 63.25 GETV genome copies (probit analysis, P ≤ 0.05). Further validation of the qRT-PCR assay using 16 spiked simulated clinical specimens showed 100% for both sensitivity and specificity. In conclusion, the qRT-PCR assay developed in this study is useful for rapid, sensitive and specific detection and quantification of GETV. |
| ArticleNumber | 17632 |
| Author | Mohamed-Romai-Noor, Noor-Adila Teoh, Boon-Teong AbuBakar, Sazaly Sam, Sing-Sin Abd-Jamil, Juraina Tan, Kim-Kee Khor, Chee-Sieng Chee, Cheah-Mun Loong, Shih-Keng |
| Author_xml | – sequence: 1 givenname: Sing-Sin surname: Sam fullname: Sam, Sing-Sin organization: Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya – sequence: 2 givenname: Boon-Teong surname: Teoh fullname: Teoh, Boon-Teong organization: Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya – sequence: 3 givenname: Cheah-Mun surname: Chee fullname: Chee, Cheah-Mun organization: Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya – sequence: 4 givenname: Noor-Adila surname: Mohamed-Romai-Noor fullname: Mohamed-Romai-Noor, Noor-Adila organization: Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya – sequence: 5 givenname: Juraina surname: Abd-Jamil fullname: Abd-Jamil, Juraina organization: Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya – sequence: 6 givenname: Shih-Keng surname: Loong fullname: Loong, Shih-Keng organization: Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya – sequence: 7 givenname: Chee-Sieng surname: Khor fullname: Khor, Chee-Sieng organization: Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya – sequence: 8 givenname: Kim-Kee orcidid: 0000-0002-5816-9146 surname: Tan fullname: Tan, Kim-Kee organization: Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya – sequence: 9 givenname: Sazaly surname: AbuBakar fullname: AbuBakar, Sazaly email: sazaly@um.edu.my organization: Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Department of Medical Microbiology, Faculty of Medicine, University of Malaya |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30518924$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9UU1PFTEUbQxGEPkDLswkbtwM9ms67caEEEUSEje6ctF0Ore8knnto-28hH9vh0FEFnTTNvecc8-95y06CDEAQu8JPiWYyc-Zk07JFhPZMoE5a8UrdEQx71rKKD148j5EJznf4Ho6qjhRb9Ahwx2RivIj9PusuZ1NKL6Y4vfQJNhDytCUZEK2ye-Kj6HdxeluC8nUgt0YHyrM2KXSuJiaEQqsv-iaCyhm0-x9mvM79NqZKcPJw32Mfn37-vP8e3v14-Ly_OyqtR3HpZVC9KBcD2K0XIKjo-q5UlYI60Y7EMb6kbhB0r4nylEJgwM7CDt0VqleGHaMvqy6u3nYwmghVPeT3iW_NelOR-P1_5XgN_o67rWgkghOq8CnB4EUb2fIRW99tjBNJkCcs6ak6wWvvboK_fgMehPnFOp4C2pZqySL4Ienjh6t_N17BdAVYFPMOYF7hBCsl3z1mq-u-er7fLWoJPmMZO9ji8tUfnqZylZqrn3CNaR_tl9g_QEG37vl |
| CitedBy_id | crossref_primary_10_1186_s12879_020_05585_4 crossref_primary_10_1007_s00705_022_05631_3 crossref_primary_10_1261_rna_079323_122 crossref_primary_10_1089_vbz_2018_2434 crossref_primary_10_1016_j_talanta_2022_123714 crossref_primary_10_1016_j_onehlt_2020_100134 crossref_primary_10_1128_jvi_01751_21 crossref_primary_10_1038_s41598_021_99734_7 crossref_primary_10_1016_j_mcp_2021_101730 crossref_primary_10_1016_j_heliyon_2024_e33432 crossref_primary_10_3389_fvets_2022_839443 crossref_primary_10_3390_ani14162309 crossref_primary_10_1128_jvi_00591_23 crossref_primary_10_3389_fmicb_2022_1009610 crossref_primary_10_3390_v14050942 |
| Cites_doi | 10.1111/tbed.12867 10.1186/s12917-017-1112-6 10.1292/jvms1939.49.1003 10.7883/yoken.65.215 10.4149/av_2012_03_265 10.1292/jvms1939.47.333 10.1292/jvms.53.855 10.1292/jvms1939.48.1191 10.1016/S0034-5288(18)32657-2 10.1016/j.mcp.2006.03.003 10.1007/s12250-012-3246-9 10.1093/nar/28.2.655 10.1292/jvms1939.43.797 10.1016/S0378-1135(01)00417-5 10.1016/0035-9203(81)90346-1 10.1292/jvms1939.44.411 10.1016/S0034-5288(18)32658-4 10.1016/0035-9203(75)90008-5 10.4269/ajtmh.1980.29.984 10.3967/bes2017.028 10.1023/A:1005079229232 10.14405/kjvr.2017.57.1.37 10.1016/S0749-0739(17)30099-8 10.3201/eid2105.141975 10.1186/s12917-016-0741-5 10.1128/JCM.00550-15 10.1007/s00705-018-3927-2 |
| ContentType | Journal Article |
| Copyright | The Author(s) 2018 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: The Author(s) 2018 – notice: 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | C6C AAYXX CITATION NPM 3V. 7X7 7XB 88A 88E 88I 8FE 8FH 8FI 8FJ 8FK ABUWG AEUYN AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M1P M2P M7P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI Q9U 7X8 5PM |
| DOI | 10.1038/s41598-018-36043-6 |
| DatabaseName | Springer Nature OA Free Journals CrossRef PubMed ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Journals ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest One Sustainability (subscription) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Korea Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Biological Sciences ProQuest Health & Medical Collection Medical Database Science Database Biological Science Database ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef PubMed Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Biology Journals (Alumni Edition) ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Sustainability ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | PubMed MEDLINE - Academic CrossRef Publicly Available Content Database |
| Database_xml | – sequence: 1 dbid: C6C name: Springer Nature OA Free Journals url: http://www.springeropen.com/ sourceTypes: Publisher – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2045-2322 |
| EndPage | 7 |
| ExternalDocumentID | PMC6281642 30518924 10_1038_s41598_018_36043_6 |
| Genre | Journal Article |
| GrantInformation_xml | – fundername: Universiti Malaya (University of Malaya) grantid: BK050-2017 funderid: https://doi.org/10.13039/501100004386 – fundername: Naval Medical Research Center (NMRC) grantid: NAMRU: J-55025-75053 funderid: https://doi.org/10.13039/100007680 – fundername: U.S. Naval Medical Research Center - Asia (Work Unit Number D-1101) and the U.S. Department of State, Biosecurity Engagement Program (NAMRU: J-55025-75053) – fundername: Universiti Malaya (University of Malaya) grantid: BK050-2017 – fundername: Naval Medical Research Center (NMRC) grantid: NAMRU: J-55025-75053 – fundername: ; grantid: BK050-2017 – fundername: ; – fundername: ; grantid: NAMRU: J-55025-75053 |
| GroupedDBID | 0R~ 3V. 4.4 53G 5VS 7X7 88A 88E 88I 8FE 8FH 8FI 8FJ AAFWJ AAJSJ AAKDD ABDBF ABUWG ACGFS ACSMW ACUHS ADBBV ADRAZ AENEX AEUYN AFKRA AJTQC ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI C6C CCPQU DIK DWQXO EBD EBLON EBS EJD ESX FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IPNFZ KQ8 LK8 M0L M1P M2P M48 M7P M~E NAO OK1 PIMPY PQQKQ PROAC PSQYO RIG RNT RNTTT RPM SNYQT UKHRP AASML AAYXX AFPKN CITATION PHGZM PHGZT NPM 7XB 8FK AARCD K9. PJZUB PKEHL PPXIY PQEST PQGLB PQUKI Q9U 7X8 5PM |
| ID | FETCH-LOGICAL-c540t-8667e9f7e6dc48ef2d97499c66cfdcb1337d1fb827719f28ebfecb6cb5c9976a3 |
| IEDL.DBID | M48 |
| ISSN | 2045-2322 |
| IngestDate | Thu Aug 21 14:12:03 EDT 2025 Thu Jul 10 17:36:55 EDT 2025 Wed Aug 13 08:09:21 EDT 2025 Thu Jan 02 23:01:22 EST 2025 Thu Apr 24 22:59:50 EDT 2025 Tue Jul 01 00:58:22 EDT 2025 Fri Feb 21 02:38:30 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | Plaque Forming Units Per Ml (PFU/ml) Alphavirus Simulated Clinical Samples Getah Virus (GETV) Nsp2 Gene |
| Language | English |
| License | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c540t-8667e9f7e6dc48ef2d97499c66cfdcb1337d1fb827719f28ebfecb6cb5c9976a3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| ORCID | 0000-0002-5816-9146 |
| OpenAccessLink | https://www.proquest.com/docview/2150518812?pq-origsite=%requestingapplication% |
| PMID | 30518924 |
| PQID | 2150518812 |
| PQPubID | 2041939 |
| PageCount | 7 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_6281642 proquest_miscellaneous_2157649765 proquest_journals_2150518812 pubmed_primary_30518924 crossref_primary_10_1038_s41598_018_36043_6 crossref_citationtrail_10_1038_s41598_018_36043_6 springer_journals_10_1038_s41598_018_36043_6 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2018-12-05 |
| PublicationDateYYYYMMDD | 2018-12-05 |
| PublicationDate_xml | – month: 12 year: 2018 text: 2018-12-05 day: 05 |
| PublicationDecade | 2010 |
| PublicationPlace | London |
| PublicationPlace_xml | – name: London – name: England |
| PublicationTitle | Scientific reports |
| PublicationTitleAbbrev | Sci Rep |
| PublicationTitleAlternate | Sci Rep |
| PublicationYear | 2018 |
| Publisher | Nature Publishing Group UK Nature Publishing Group |
| Publisher_xml | – name: Nature Publishing Group UK – name: Nature Publishing Group |
| References | KawamuraHA fatal case in newborn piglets with Getah virus infection: pathogenicity of the isolateNihon Juigaku Zasshi198749100310071:STN:280:DyaL1c7hvFynug%3D%3D10.1292/jvms1939.49.1003 LiYYIdentification of a newly isolated Getah Virus in the China-Laos border, ChinaBiomed Environ Sci20173021021410.3967/bes2017.02828427491 WadaREquine Getah virus infection: pathological study of horses experimentally infected with the MI-110strainNihon Juigaku Zasshi1982444114181:STN:280:DyaL3s%2FjsVGisQ%3D%3D10.1292/jvms1939.44.411 ImagawaHSero-epizootiological survey on Getah virus infection in light horses in JapanNihon Juigaku Zasshi1981437978021:STN:280:DyaL383hslKgsg%3D%3D10.1292/jvms1939.43.797 BannaiHGeospatial and temporal associations of Getah virus circulation among pigs and horses around the perimeter of outbreaks in Japanese racehorses in 2014 and 2015BMC Vet Res2017131871:CAS:528:DC%2BC1cXitVagu7nL10.1186/s12917-017-1112-6286294065477264 ChastelCRageeuJIsolation of arbovirus in Cambodia from naturally infected mosquitoesMed Trop (Mars)1966263914001:STN:280:DyaF2s%2FhvVCksQ%3D%3D SeoHJCharacterization of recent Getah virus isolates from South KoreaActa Virol20125626526710.4149/av_2012_03_265 KamadaMEquine Getah virus infection: isolation of the virus from racehorses during an enzootic in JapanAm J Trop Med Hyg1980299849881:STN:280:DyaL3M%2FmtVSlsg%3D%3D10.4269/ajtmh.1980.29.984 YangTAn outbreak of Getah virus infection among pigs in China, 2017Transbound Emerg Dis2018656326371:CAS:528:DC%2BC1cXoslyks7g%3D10.1111/tbed.1286729575687 LiXDQiuFXYangHRaoYNCalisherCHIsolation of Getah virus from mosquitos collected on Hainan Island, China, and results of a serosurveySoutheast Asian J Trop Med Public Health1992237307341:STN:280:DyaK3s3hvF2jsA%3D%3D1338481 NemotoMGetah virus infection among racehorses, Japan, 2014Emerg Infect Dis2015218838851:CAS:528:DC%2BC28XitVKitr7M10.3201/eid2105.141975258981814412242 SimpsonDIArbovirus infections in Sarawak, October 1968-February 1970: Getah virus isolations from mosquitoesTrans R Soc Trop Med Hyg19756935381:STN:280:DyaE2M7pvFCqsg%3D%3D10.1016/0035-9203(75)90008-5 FukunagaYKumanomidoTKamadaMGetah virus as an equine pathogenVet Clin North Am Equine Pract2000166056171:STN:280:DC%2BD3M7ls1Ojsw%3D%3D10.1016/S0749-0739(17)30099-8 BannaiHA 2015 outbreak of Getah virus infection occurring among Japanese racehorses sequentially to an outbreak in 2014 at the same siteBMC Vet Res201612981:CAS:528:DC%2BC2sXnsVertbc%3D10.1186/s12917-016-0741-5272866584902926 YaoYNellakerCKarlssonHEvaluation of minor groove binding probe and Taqman probe PCR assays: Influence of mismatches and template complexity on quantificationMol Cell Probes2006203113161:CAS:528:DC%2BD28XntlSqtbY%3D10.1016/j.mcp.2006.03.00316704921 DongDSimultaneous detection of three arboviruses using a triplex RT-PCR: enzyme hybridization assayVirol Sin2012271791861:CAS:528:DC%2BC38XosFCrsrg%3D10.1007/s12250-012-3246-9226844723724924 FengYDistribution of mosquitoes and mosquito-borne viruses along the China-Myanmar border in Yunnan ProvinceJpn J Infect Dis20126521522110.7883/yoken.65.215 KonoYSentsuiHItoYAn epidemic of Getah virus infection among racehorses: properties of the virusRes Vet Sci1980291621671:STN:280:DyaL3M7isFGgtw%3D%3D10.1016/S0034-5288(18)32658-4 BrownCMTimoneyPJGetah virus infection of Indian horsesTrop Anim Health Prod1998302412521:STN:280:DyaK1cvjtlChtw%3D%3D10.1023/A:1005079229232 KumanomidoTGetah virus isolations from mosquitoes collected at two horse habitations in the western areas of JapanNihon Juigaku Zasshi198648119111971:STN:280:DyaL2s7kt1Citw%3D%3D10.1292/jvms1939.48.1191 WekesaSNInoshimaYMurakamiKSentsuiHGenomic analysis of some Japanese isolates of Getah virusVet Microbiol2001831371461:CAS:528:DC%2BD3MXms12ns70%3D10.1016/S0378-1135(01)00417-5 Berge, T. Getah. International Catalogue of Arboviruses. 2nd edn, (Washington DC: US Department of Health, Education, and Welfare, 1975). LeeSHYangDKKimHHChoiSSChoISEstablishment of reverse transcription polymerase chain reaction for detection of Getah virus infection in livestockKorean J Vet Res201757374210.14405/kjvr.2017.57.1.37 KsiazekTGTrosperJHCrossJHBasaca-SevillaVIsolation of Getah virus from Nueva Ecija Province, Republic of the PhilippinesTrans R Soc Trop Med Hyg1981753123131:STN:280:DyaL38%2Fmt12htA%3D%3D10.1016/0035-9203(81)90346-1 BannaiHEpizootiological investigation of Getah virus infection among racehorses in Japan in 2014J Clin Microbiol2015532286229110.1128/JCM.00550-15259724254473224 MarchetteNJRudnickAGarciaRMacVeanDWAlphaviruses in Peninusular Malaysia: I. Virus isolations and animal serologySoutheast Asian J Trop Med Public Health197893173291:STN:280:DyaE1M7lvFOnsA%3D%3D34888 KutyavinIV3′-minor groove binder-DNA probes increase sequence specificity at PCR extension temperaturesNucleic Acids Res2000286556611:CAS:528:DC%2BD3cXhtVSkur8%3D10.1093/nar/28.2.655 MarchetteNJRudnickAGarciaRAlphaviruses in Peninsular Malaysia: II. Serological evidence of human infectionSoutheast Asian J Trop Med Public Health19801114231:STN:280:DyaL3c3lvVOnsw%3D%3D7403943 ShiNingLiuHaoLiLi-xiaHuBoZhangLeiZhaoChuan-fangDengXiao-yuLiXin-tongXueXiang-hongBaiXueZhangHai-lingLuRong-guangLianShi-zhenWangYangYanMing-haoYanXi-junDevelopment of a TaqMan probe-based quantitative reverse transcription PCR assay for detection of Getah virus RNAArchives of Virology201816310287728811:CAS:528:DC%2BC1cXhtlSru7bL10.1007/s00705-018-3927-2 SentsuiHKonoYAn epidemic of Getah virus infection among racehorses: isolation of the virusRes Vet Sci1980291571611:STN:280:DyaL3M7isFGgtg%3D%3D10.1016/S0034-5288(18)32657-2 KamadaMIntranasal infection of Getah virus in experimental horsesJ Vet Med Sci1991538558581:STN:280:DyaK38%2FovFaktg%3D%3D10.1292/jvms.53.855 SentsuiHKonoYReappearance of Getah virus infection among horses in JapanNihon Juigaku Zasshi1985473333351:STN:280:DyaL2M3jvVCrtw%3D%3D10.1292/jvms1939.47.333 NJ Marchette (36043_CR11) 1978; 9 CM Brown (36043_CR15) 1998; 30 36043_CR1 NJ Marchette (36043_CR10) 1980; 11 YY Li (36043_CR6) 2017; 30 H Bannai (36043_CR18) 2015; 53 H Imagawa (36043_CR17) 1981; 43 M Nemoto (36043_CR20) 2015; 21 Y Fukunaga (36043_CR23) 2000; 16 H Sentsui (36043_CR14) 1985; 47 R Wada (36043_CR24) 1982; 44 Y Kono (36043_CR12) 1980; 29 H Kawamura (36043_CR21) 1987; 49 H Bannai (36043_CR19) 2016; 12 C Chastel (36043_CR2) 1966; 26 Y Yao (36043_CR31) 2006; 20 H Bannai (36043_CR26) 2017; 13 SN Wekesa (36043_CR29) 2001; 83 SH Lee (36043_CR28) 2017; 57 XD Li (36043_CR9) 1992; 23 T Yang (36043_CR22) 2018; 65 M Kamada (36043_CR25) 1991; 53 TG Ksiazek (36043_CR4) 1981; 75 D Dong (36043_CR27) 2012; 27 HJ Seo (36043_CR7) 2012; 56 M Kamada (36043_CR16) 1980; 29 IV Kutyavin (36043_CR30) 2000; 28 H Sentsui (36043_CR13) 1980; 29 DI Simpson (36043_CR8) 1975; 69 Y Feng (36043_CR3) 2012; 65 T Kumanomido (36043_CR5) 1986; 48 Ning Shi (36043_CR32) 2018; 163 22627302 - Jpn J Infect Dis. 2012;65(3):215-21 11557154 - Vet Microbiol. 2001 Nov 8;83(2):137-46 34888 - Southeast Asian J Trop Med Public Health. 1978 Sep;9(3):317-29 6254385 - Am J Trop Med Hyg. 1980 Sep;29(5):984-8 238314 - Trans R Soc Trop Med Hyg. 1975;69(1):35-8 22684472 - Virol Sin. 2012 Jun;27(3):179-86 29987379 - Arch Virol. 2018 Jul 9;:null 23043609 - Acta Virol. 2012;56(3):265-7 29575687 - Transbound Emerg Dis. 2018 Jun;65(3):632-637 1661175 - J Vet Med Sci. 1991 Oct;53(5):855-8 10606668 - Nucleic Acids Res. 2000 Jan 15;28(2):655-61 28629406 - BMC Vet Res. 2017 Jun 19;13(1):187 1338481 - Southeast Asian J Trop Med Public Health. 1992 Dec;23(4):730-4 5919700 - Med Trop (Mars). 1966 Jul-Aug;26(4):391-400 2828730 - Nihon Juigaku Zasshi. 1987 Dec;49(6):1003-7 28427491 - Biomed Environ Sci. 2017 Mar;30(3):210-214 25898181 - Emerg Infect Dis. 2015 May;21(5):883-5 16704921 - Mol Cell Probes. 2006 Oct;20(5):311-6 6258205 - Res Vet Sci. 1980 Sep;29(2):162-7 9760716 - Trop Anim Health Prod. 1998 Aug;30(4):241-52 7403943 - Southeast Asian J Trop Med Public Health. 1980 Mar;11(1):14-23 25972425 - J Clin Microbiol. 2015 Jul;53(7):2286-91 6117960 - Trans R Soc Trop Med Hyg. 1981;75(2):312-3 6258204 - Res Vet Sci. 1980 Sep;29(2):157-61 11219353 - Vet Clin North Am Equine Pract. 2000 Dec;16(3):605-17 2989599 - Nihon Juigaku Zasshi. 1985 Apr;47(2):333-5 2881021 - Nihon Juigaku Zasshi. 1986 Dec;48(6):1191-7 6290732 - Nihon Juigaku Zasshi. 1982 Jun;44(3):411-8 6283217 - Nihon Juigaku Zasshi. 1981 Dec;43(6):797-802 27286658 - BMC Vet Res. 2016 Jun 10;12:98 |
| References_xml | – reference: KsiazekTGTrosperJHCrossJHBasaca-SevillaVIsolation of Getah virus from Nueva Ecija Province, Republic of the PhilippinesTrans R Soc Trop Med Hyg1981753123131:STN:280:DyaL38%2Fmt12htA%3D%3D10.1016/0035-9203(81)90346-1 – reference: KamadaMEquine Getah virus infection: isolation of the virus from racehorses during an enzootic in JapanAm J Trop Med Hyg1980299849881:STN:280:DyaL3M%2FmtVSlsg%3D%3D10.4269/ajtmh.1980.29.984 – reference: ShiNingLiuHaoLiLi-xiaHuBoZhangLeiZhaoChuan-fangDengXiao-yuLiXin-tongXueXiang-hongBaiXueZhangHai-lingLuRong-guangLianShi-zhenWangYangYanMing-haoYanXi-junDevelopment of a TaqMan probe-based quantitative reverse transcription PCR assay for detection of Getah virus RNAArchives of Virology201816310287728811:CAS:528:DC%2BC1cXhtlSru7bL10.1007/s00705-018-3927-2 – reference: DongDSimultaneous detection of three arboviruses using a triplex RT-PCR: enzyme hybridization assayVirol Sin2012271791861:CAS:528:DC%2BC38XosFCrsrg%3D10.1007/s12250-012-3246-9226844723724924 – reference: ImagawaHSero-epizootiological survey on Getah virus infection in light horses in JapanNihon Juigaku Zasshi1981437978021:STN:280:DyaL383hslKgsg%3D%3D10.1292/jvms1939.43.797 – reference: ChastelCRageeuJIsolation of arbovirus in Cambodia from naturally infected mosquitoesMed Trop (Mars)1966263914001:STN:280:DyaF2s%2FhvVCksQ%3D%3D – reference: KutyavinIV3′-minor groove binder-DNA probes increase sequence specificity at PCR extension temperaturesNucleic Acids Res2000286556611:CAS:528:DC%2BD3cXhtVSkur8%3D10.1093/nar/28.2.655 – reference: WekesaSNInoshimaYMurakamiKSentsuiHGenomic analysis of some Japanese isolates of Getah virusVet Microbiol2001831371461:CAS:528:DC%2BD3MXms12ns70%3D10.1016/S0378-1135(01)00417-5 – reference: LiYYIdentification of a newly isolated Getah Virus in the China-Laos border, ChinaBiomed Environ Sci20173021021410.3967/bes2017.02828427491 – reference: KawamuraHA fatal case in newborn piglets with Getah virus infection: pathogenicity of the isolateNihon Juigaku Zasshi198749100310071:STN:280:DyaL1c7hvFynug%3D%3D10.1292/jvms1939.49.1003 – reference: BannaiHA 2015 outbreak of Getah virus infection occurring among Japanese racehorses sequentially to an outbreak in 2014 at the same siteBMC Vet Res201612981:CAS:528:DC%2BC2sXnsVertbc%3D10.1186/s12917-016-0741-5272866584902926 – reference: MarchetteNJRudnickAGarciaRAlphaviruses in Peninsular Malaysia: II. Serological evidence of human infectionSoutheast Asian J Trop Med Public Health19801114231:STN:280:DyaL3c3lvVOnsw%3D%3D7403943 – reference: MarchetteNJRudnickAGarciaRMacVeanDWAlphaviruses in Peninusular Malaysia: I. Virus isolations and animal serologySoutheast Asian J Trop Med Public Health197893173291:STN:280:DyaE1M7lvFOnsA%3D%3D34888 – reference: YangTAn outbreak of Getah virus infection among pigs in China, 2017Transbound Emerg Dis2018656326371:CAS:528:DC%2BC1cXoslyks7g%3D10.1111/tbed.1286729575687 – reference: BannaiHGeospatial and temporal associations of Getah virus circulation among pigs and horses around the perimeter of outbreaks in Japanese racehorses in 2014 and 2015BMC Vet Res2017131871:CAS:528:DC%2BC1cXitVagu7nL10.1186/s12917-017-1112-6286294065477264 – reference: KumanomidoTGetah virus isolations from mosquitoes collected at two horse habitations in the western areas of JapanNihon Juigaku Zasshi198648119111971:STN:280:DyaL2s7kt1Citw%3D%3D10.1292/jvms1939.48.1191 – reference: BrownCMTimoneyPJGetah virus infection of Indian horsesTrop Anim Health Prod1998302412521:STN:280:DyaK1cvjtlChtw%3D%3D10.1023/A:1005079229232 – reference: WadaREquine Getah virus infection: pathological study of horses experimentally infected with the MI-110strainNihon Juigaku Zasshi1982444114181:STN:280:DyaL3s%2FjsVGisQ%3D%3D10.1292/jvms1939.44.411 – reference: NemotoMGetah virus infection among racehorses, Japan, 2014Emerg Infect Dis2015218838851:CAS:528:DC%2BC28XitVKitr7M10.3201/eid2105.141975258981814412242 – reference: KamadaMIntranasal infection of Getah virus in experimental horsesJ Vet Med Sci1991538558581:STN:280:DyaK38%2FovFaktg%3D%3D10.1292/jvms.53.855 – reference: YaoYNellakerCKarlssonHEvaluation of minor groove binding probe and Taqman probe PCR assays: Influence of mismatches and template complexity on quantificationMol Cell Probes2006203113161:CAS:528:DC%2BD28XntlSqtbY%3D10.1016/j.mcp.2006.03.00316704921 – reference: FengYDistribution of mosquitoes and mosquito-borne viruses along the China-Myanmar border in Yunnan ProvinceJpn J Infect Dis20126521522110.7883/yoken.65.215 – reference: SentsuiHKonoYReappearance of Getah virus infection among horses in JapanNihon Juigaku Zasshi1985473333351:STN:280:DyaL2M3jvVCrtw%3D%3D10.1292/jvms1939.47.333 – reference: SeoHJCharacterization of recent Getah virus isolates from South KoreaActa Virol20125626526710.4149/av_2012_03_265 – reference: BannaiHEpizootiological investigation of Getah virus infection among racehorses in Japan in 2014J Clin Microbiol2015532286229110.1128/JCM.00550-15259724254473224 – reference: Berge, T. Getah. International Catalogue of Arboviruses. 2nd edn, (Washington DC: US Department of Health, Education, and Welfare, 1975). – reference: SimpsonDIArbovirus infections in Sarawak, October 1968-February 1970: Getah virus isolations from mosquitoesTrans R Soc Trop Med Hyg19756935381:STN:280:DyaE2M7pvFCqsg%3D%3D10.1016/0035-9203(75)90008-5 – reference: LiXDQiuFXYangHRaoYNCalisherCHIsolation of Getah virus from mosquitos collected on Hainan Island, China, and results of a serosurveySoutheast Asian J Trop Med Public Health1992237307341:STN:280:DyaK3s3hvF2jsA%3D%3D1338481 – reference: SentsuiHKonoYAn epidemic of Getah virus infection among racehorses: isolation of the virusRes Vet Sci1980291571611:STN:280:DyaL3M7isFGgtg%3D%3D10.1016/S0034-5288(18)32657-2 – reference: LeeSHYangDKKimHHChoiSSChoISEstablishment of reverse transcription polymerase chain reaction for detection of Getah virus infection in livestockKorean J Vet Res201757374210.14405/kjvr.2017.57.1.37 – reference: FukunagaYKumanomidoTKamadaMGetah virus as an equine pathogenVet Clin North Am Equine Pract2000166056171:STN:280:DC%2BD3M7ls1Ojsw%3D%3D10.1016/S0749-0739(17)30099-8 – reference: KonoYSentsuiHItoYAn epidemic of Getah virus infection among racehorses: properties of the virusRes Vet Sci1980291621671:STN:280:DyaL3M7isFGgtw%3D%3D10.1016/S0034-5288(18)32658-4 – volume: 65 start-page: 632 year: 2018 ident: 36043_CR22 publication-title: Transbound Emerg Dis doi: 10.1111/tbed.12867 – volume: 13 start-page: 187 year: 2017 ident: 36043_CR26 publication-title: BMC Vet Res doi: 10.1186/s12917-017-1112-6 – volume: 49 start-page: 1003 year: 1987 ident: 36043_CR21 publication-title: Nihon Juigaku Zasshi doi: 10.1292/jvms1939.49.1003 – volume: 65 start-page: 215 year: 2012 ident: 36043_CR3 publication-title: Jpn J Infect Dis doi: 10.7883/yoken.65.215 – ident: 36043_CR1 – volume: 56 start-page: 265 year: 2012 ident: 36043_CR7 publication-title: Acta Virol doi: 10.4149/av_2012_03_265 – volume: 47 start-page: 333 year: 1985 ident: 36043_CR14 publication-title: Nihon Juigaku Zasshi doi: 10.1292/jvms1939.47.333 – volume: 53 start-page: 855 year: 1991 ident: 36043_CR25 publication-title: J Vet Med Sci doi: 10.1292/jvms.53.855 – volume: 48 start-page: 1191 year: 1986 ident: 36043_CR5 publication-title: Nihon Juigaku Zasshi doi: 10.1292/jvms1939.48.1191 – volume: 29 start-page: 157 year: 1980 ident: 36043_CR13 publication-title: Res Vet Sci doi: 10.1016/S0034-5288(18)32657-2 – volume: 20 start-page: 311 year: 2006 ident: 36043_CR31 publication-title: Mol Cell Probes doi: 10.1016/j.mcp.2006.03.003 – volume: 27 start-page: 179 year: 2012 ident: 36043_CR27 publication-title: Virol Sin doi: 10.1007/s12250-012-3246-9 – volume: 23 start-page: 730 year: 1992 ident: 36043_CR9 publication-title: Southeast Asian J Trop Med Public Health – volume: 11 start-page: 14 year: 1980 ident: 36043_CR10 publication-title: Southeast Asian J Trop Med Public Health – volume: 28 start-page: 655 year: 2000 ident: 36043_CR30 publication-title: Nucleic Acids Res doi: 10.1093/nar/28.2.655 – volume: 43 start-page: 797 year: 1981 ident: 36043_CR17 publication-title: Nihon Juigaku Zasshi doi: 10.1292/jvms1939.43.797 – volume: 83 start-page: 137 year: 2001 ident: 36043_CR29 publication-title: Vet Microbiol doi: 10.1016/S0378-1135(01)00417-5 – volume: 75 start-page: 312 year: 1981 ident: 36043_CR4 publication-title: Trans R Soc Trop Med Hyg doi: 10.1016/0035-9203(81)90346-1 – volume: 44 start-page: 411 year: 1982 ident: 36043_CR24 publication-title: Nihon Juigaku Zasshi doi: 10.1292/jvms1939.44.411 – volume: 29 start-page: 162 year: 1980 ident: 36043_CR12 publication-title: Res Vet Sci doi: 10.1016/S0034-5288(18)32658-4 – volume: 69 start-page: 35 year: 1975 ident: 36043_CR8 publication-title: Trans R Soc Trop Med Hyg doi: 10.1016/0035-9203(75)90008-5 – volume: 29 start-page: 984 year: 1980 ident: 36043_CR16 publication-title: Am J Trop Med Hyg doi: 10.4269/ajtmh.1980.29.984 – volume: 30 start-page: 210 year: 2017 ident: 36043_CR6 publication-title: Biomed Environ Sci doi: 10.3967/bes2017.028 – volume: 9 start-page: 317 year: 1978 ident: 36043_CR11 publication-title: Southeast Asian J Trop Med Public Health – volume: 30 start-page: 241 year: 1998 ident: 36043_CR15 publication-title: Trop Anim Health Prod doi: 10.1023/A:1005079229232 – volume: 57 start-page: 37 year: 2017 ident: 36043_CR28 publication-title: Korean J Vet Res doi: 10.14405/kjvr.2017.57.1.37 – volume: 26 start-page: 391 year: 1966 ident: 36043_CR2 publication-title: Med Trop (Mars) – volume: 16 start-page: 605 year: 2000 ident: 36043_CR23 publication-title: Vet Clin North Am Equine Pract doi: 10.1016/S0749-0739(17)30099-8 – volume: 21 start-page: 883 year: 2015 ident: 36043_CR20 publication-title: Emerg Infect Dis doi: 10.3201/eid2105.141975 – volume: 12 start-page: 98 year: 2016 ident: 36043_CR19 publication-title: BMC Vet Res doi: 10.1186/s12917-016-0741-5 – volume: 53 start-page: 2286 year: 2015 ident: 36043_CR18 publication-title: J Clin Microbiol doi: 10.1128/JCM.00550-15 – volume: 163 start-page: 2877 issue: 10 year: 2018 ident: 36043_CR32 publication-title: Archives of Virology doi: 10.1007/s00705-018-3927-2 – reference: 10606668 - Nucleic Acids Res. 2000 Jan 15;28(2):655-61 – reference: 238314 - Trans R Soc Trop Med Hyg. 1975;69(1):35-8 – reference: 22684472 - Virol Sin. 2012 Jun;27(3):179-86 – reference: 6117960 - Trans R Soc Trop Med Hyg. 1981;75(2):312-3 – reference: 1661175 - J Vet Med Sci. 1991 Oct;53(5):855-8 – reference: 16704921 - Mol Cell Probes. 2006 Oct;20(5):311-6 – reference: 6290732 - Nihon Juigaku Zasshi. 1982 Jun;44(3):411-8 – reference: 28629406 - BMC Vet Res. 2017 Jun 19;13(1):187 – reference: 23043609 - Acta Virol. 2012;56(3):265-7 – reference: 6258204 - Res Vet Sci. 1980 Sep;29(2):157-61 – reference: 29575687 - Transbound Emerg Dis. 2018 Jun;65(3):632-637 – reference: 5919700 - Med Trop (Mars). 1966 Jul-Aug;26(4):391-400 – reference: 11557154 - Vet Microbiol. 2001 Nov 8;83(2):137-46 – reference: 1338481 - Southeast Asian J Trop Med Public Health. 1992 Dec;23(4):730-4 – reference: 2989599 - Nihon Juigaku Zasshi. 1985 Apr;47(2):333-5 – reference: 2828730 - Nihon Juigaku Zasshi. 1987 Dec;49(6):1003-7 – reference: 9760716 - Trop Anim Health Prod. 1998 Aug;30(4):241-52 – reference: 25972425 - J Clin Microbiol. 2015 Jul;53(7):2286-91 – reference: 6258205 - Res Vet Sci. 1980 Sep;29(2):162-7 – reference: 6254385 - Am J Trop Med Hyg. 1980 Sep;29(5):984-8 – reference: 28427491 - Biomed Environ Sci. 2017 Mar;30(3):210-214 – reference: 25898181 - Emerg Infect Dis. 2015 May;21(5):883-5 – reference: 29987379 - Arch Virol. 2018 Jul 9;:null – reference: 7403943 - Southeast Asian J Trop Med Public Health. 1980 Mar;11(1):14-23 – reference: 27286658 - BMC Vet Res. 2016 Jun 10;12:98 – reference: 6283217 - Nihon Juigaku Zasshi. 1981 Dec;43(6):797-802 – reference: 34888 - Southeast Asian J Trop Med Public Health. 1978 Sep;9(3):317-29 – reference: 2881021 - Nihon Juigaku Zasshi. 1986 Dec;48(6):1191-7 – reference: 22627302 - Jpn J Infect Dis. 2012;65(3):215-21 – reference: 11219353 - Vet Clin North Am Equine Pract. 2000 Dec;16(3):605-17 |
| SSID | ssj0000529419 |
| Score | 2.333097 |
| Snippet | Getah virus (GETV), a mosquito-borne alphavirus, is an emerging animal pathogen causing outbreaks among racehorses and pigs. Early detection of the GETV... |
| SourceID | pubmedcentral proquest pubmed crossref springer |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 17632 |
| SubjectTerms | 13 13/106 45/90 631/326/2521 631/326/596/1282 Genomes Humanities and Social Sciences multidisciplinary Nucleic acids Outbreaks Polymerase chain reaction Primers Reverse transcription Ribonucleic acid RNA Science Science (multidisciplinary) |
| SummonAdditionalLinks | – databaseName: Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfR1da9RAcNCK4Iu0fjW2ygq-6dJmk-zHkxSxLYI-WTjwIWS_uIOSXC85of_emXyVs9inEHaWZHdm53NnBuBjZXUmpYocD7XkuXAFt7ZQ3DutkIB8NI5cAz9-ysur_PuiWIwOt3a8VjnxxJ5R-8aRj_wERdMpFQ9LxZf1DaeuURRdHVtoPIYnVLqMqFot1OxjoShWnpoxV-Y00yctyivKKUs1zyQFnuWuPLqnZN6_K_lPwLSXQ-f78HxUINnZgPEDeBTqF_B0aCl5-xJ-n7GbbVX3qWPIyBhVaNq0gXUkkyYOwdfN9S15o3DALatVjWBDggNDHZb50IXhrYnsInTVkv1ZbbbtK7g6__br6yUfGyhwh4pYxzViIZiogvQu1yEKj9aDMU5KF72zaJ4qn0arhVKpiUIHG4Oz0tnCGVRTquw17NVNHQ6BIT-1maioNEyRR4NP44NWAvUZNHELnUA6bWPpxuri1OTiuuyj3Jkuh60vcevLfutLmcCnec56qK3xIPTxhJ1yPGdteUcVCXyYh_GEUNijqkOz7WGUzHE9RQJvBmTOn8toNpqgCagdNM8AVH17d6ReLfsq3FJoNDXxu58ngrj7rf-v4u3DqziCZ4KIk-7LFMew12224R1qPZ1935P2X08vAIw priority: 102 providerName: ProQuest – databaseName: Springer Nature HAS Fully OA dbid: AAJSJ link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Za9wwEB7ShEBfQq62bg4UyFsiGsu2jsel5GAheUkCgTwYS5bYheDd7noD-fcdyUfZHIU8GaMRkkYjaUYz8wnguNAy4Vw4ioua05SZjGqdCVoaKVCASqeMvxq4vuFX9-nwIXtYAdblwoSg_QBpGbbpLjrs1xwPGp8MFkuacO8x5l9gzUO1o2yvDQbD22F_s-J9V2ms2gyZs0S-U3n5FHqjWr6NkHzlJg2nz8UmbLRqIxk0Hd2CFVttw3rzkOTLDjwOyJ9FUYWEMdy-iMdlms0tqf1J1O0LdDp5evF3UFhgRsW4QrImrYGg5kpKW9vmb-LIpa2LEXkezxbzXbi_OL_7fUXbZxOoQfWrphJ5b5UTlpcmldaxEm0GpQznxpVGo1EqythpyYSIlWPSameN5kZnRqFyUiTfYLWaVPYHENxFdcIKDwiTpU7hV5VWCoZaDBq2mYwg7tiYmxZT3D9t8ZQH33Yi84b1ObI-D6zPeQQnfZ1pg6jxX-r9bnbydnXNc1RTzjyQXMwiOOqLcV14Z0dR2cki0Aie4niyCL43k9k3l_jaaHhGIJamuSfwmNvLJdV4FLC3OZNoYGK7p51A_OvWx6P4-TnyPfjKvLD6qJlsH1br2cIeoO5T68NW2P8CXeL_vg priority: 102 providerName: Springer Nature |
| Title | A quantitative reverse transcription-polymerase chain reaction for detection of Getah virus |
| URI | https://link.springer.com/article/10.1038/s41598-018-36043-6 https://www.ncbi.nlm.nih.gov/pubmed/30518924 https://www.proquest.com/docview/2150518812 https://www.proquest.com/docview/2157649765 https://pubmed.ncbi.nlm.nih.gov/PMC6281642 |
| Volume | 8 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3da9swED_6wUZfyj47b13QYG-bNizb-ngYIw3tSqBlbAsE9mAsWSKB4LSJM5b_vifLzkg_9mRsyVjy3el-p9PdAbwvtEw4F46iUHOaMpNRrTNBSyMFMlDplPFbAxeX_HyUDsfZeAe6ckftD1zea9r5elKjxezT3-v1VxT4LyFkXH5eohLygWKxpAn33mS-C_uomYSvaHDRwv2Q65upNFZt7Mz9rx7AYxSBWCqWbquqO_jz7jHKW77URkWdPYHDFluSfmCGp7Bjq2fwKFSbXD-H331yvSqqJqoM1zjikzctlpbUXl11iwe9ms_WfqMKG8ykmFbYLcQ-EIS3pLS1DXdzR77ZupiQP9PFavkCRmenvwbntK2tQA1itJpKJJBVTlhemlRax0o0LJQynBtXGo2WqyhjpyUTIlaOSaudNZobnRmFCKZIXsJeNa_sKyC41OqEFT5rTJY6hVdVWikYQh20fjMZQdz9xty0icd9_YtZ3jjAE5kHKuRIhbyhQs4j-LB55yqk3fhv7-OOOnnHQTliGU9HBDARvNs0o_B4j0hR2fmq6SN4ivPJIjgKxNx8ruOCCMQWmTcdfGLu7ZZqOmkSdHMm0QrF737sGOLfsB6exesHh_AGDphnUX-KJjuGvXqxsm8RC9W6B7tiLHqw3-8Pfw7xenJ6-f0HPh3wQa_ZX-g1InADsfMJgA |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtR3LbtQwcFSKEFwqniWlQJDgBFY3TmI7B4QqoGzp49RKK3EIsWNrV6qS7SYL2p_iG5nJq1oqeuspijxO7PF4Hh7PDMDbTKtQCOkYbmrBIm5ipnUsWW6URALKXWLoaODkVIzPo--TeLIBf_pYGLpW2fPEhlHnpaEz8j0UTSNKHhbwT_NLRlWjyLval9BoyeLIrn6jyVZ9PPyC6_uO84OvZ5_HrKsqwAxqJzVTODSbOGlFbiJlHc9RpU4SI4RxudFos8k8cFpxKYPEcWW1s0YLo2OToOzOQvzuHbiLgndExp6cyOFMh7xmUZB0sTmjUO1VKB8phi1QLBTk6Bbr8u-aUnv9buY_DtpG7h08hK1OYfX3Wwp7BBu2eAz32hKWqyfwY9-_XGZFE6qGjNOnjFCLyvo1ycCeI7F5ebGi0y9sMNNsViBYG1Dho87s57a27Vvp_G-2zqb-r9liWT2F81tB7TPYLMrCPgcf-bcOeUapaOLIJfhMcqskR_0JTepYeRD0aExNl82cimpcpI1XPVRpi_oUUZ82qE-FB--HPvM2l8eN0Lv96qTdvq7SKyr04M3QjDuS3CxZYctlAyNFhPOJPdhuF3P4XUi90eT1QK4t8wBA2b7XW4rZtMn6LbhC0xb_-6EniKth_X8WOzfP4jXcH5-dHKfHh6dHL-ABJ0KluzrxLmzWi6V9iRpXrV81ZO7Dz9veV38B-14_IQ |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtR1db9Mw8DQ6gXhBfBMYECR4AquLk9jOA0KDrWwMqgkxadIeQuzYaqUp6doU1L_Gr-MuX1OZ2Nueosh2Yp_v0-e7A3idaRUKIR1DohYs4iZmWseS5UZJRKDcJYaOBr6Nxf5x9OUkPtmAP10sDF2r7Hhizajz0tAZ-RBF0zYlDwv40LXXIo52Rx9m54wqSJGntSun0aDIoV39RvNt8f5gF_f6DeejvR-f9llbYYAZ1FQqpnCaNnHSitxEyjqeo3qdJEYI43Kj0X6TeeC04lIGiePKameNFkbHJkE5noX43RuwKckqGsDmx73x0ff-hId8aFGQtJE626EaLlBaUkRboFgoyO0t1qXhJRX38k3Nf9y1tRQc3YU7rfrq7zT4dg82bHEfbjYFLVcP4HTHP19mRR24hmzUp_xQ84X1K5KIHX9is_JsRWdh2GAm2bTAbk14hY8atJ_byjZvpfM_2yqb-L-m8-XiIRxfC3AfwaAoC_sEfOTmOuQZJaaJI5fgM8mtkhy1KTSwY-VB0IExNW1ucyqxcZbWPvZQpQ3oUwR9WoM-FR687cfMmsweV_be6nYnbal8kV7gpAev-makT3K6ZIUtl3UfKSJcT-zB42Yz-9-FNBoNYA_k2jb3HSj393pLMZ3UOcAFV2jo4n_fdQhxMa3_r-Lp1at4CbeQptKvB-PDZ3CbE57SxZ14CwbVfGmfo_pV6Rctnvvw87pJ6y8xzkS8 |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+quantitative+reverse+transcription-polymerase+chain+reaction+for+detection+of+Getah+virus&rft.jtitle=Scientific+reports&rft.au=Sam%2C+Sing-Sin&rft.au=Teoh%2C+Boon-Teong&rft.au=Chee%2C+Cheah-Mun&rft.au=Mohamed-Romai-Noor%2C+Noor-Adila&rft.date=2018-12-05&rft.eissn=2045-2322&rft.volume=8&rft.issue=1&rft.spage=17632&rft_id=info:doi/10.1038%2Fs41598-018-36043-6&rft_id=info%3Apmid%2F30518924&rft.externalDocID=30518924 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon |