MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy

• Published in: Nature communications Vol. 11; no. 1; pp. 2148 - 16
• Main Authors: Wang, Yi, Luo, Wu, Han, Jibo, Khan, Zia A., Fang, Qilu, Jin, Yiyi, Chen, Xuemei, Zhang, Yali, Wang, Meihong, Qian, Jianchang, Huang, Weijian, Lum, Hazel, Wu, Gaojun, Liang, Guang
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.05.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/51; 13/95; 692/163/2743/137/138; 692/699/249/2510; 692/699/75/74/1540; 82/29; Activation; Advanced glycosylation end products; Age; Animals; Blotting, Western; Calorimetry; Cardiomyopathy; Cell Line; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - immunology; Diabetes Mellitus, Experimental - metabolism; Diabetic Cardiomyopathies - immunology; Diabetic Cardiomyopathies - metabolism; Glycation End Products, Advanced - metabolism; Glycosylation; Heart; Humanities and Social Sciences; Humans; Hyperglycemia; Immunoprecipitation; Inflammation; Lymphocyte Antigen 96 - metabolism; Male; Mice; Mice, Inbred C57BL; multidisciplinary; Rats; Rats, Sprague-Dawley; Receptors; Science; Science (multidisciplinary); Signal Transduction - genetics; Signal Transduction - physiology; TLR4 protein; Toll-Like Receptor 4 - metabolism; Toll-like receptors
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-15978-3

Hyperglycemia activates toll-like receptor 4 (TLR4) to induce inflammation in diabetic cardiomyopathy (DCM). However, the mechanisms of TLR4 activation remain unclear. Here we examine the role of myeloid differentiation 2 (MD2), a co-receptor of TLR4, in high glucose (HG)- and diabetes-induced inflammatory cardiomyopathy. We show increased MD2 in heart tissues of diabetic mice and serum of human diabetic subjects. MD2 deficiency in mice inhibits TLR4 pathway activation, which correlates with reduced myocardial remodeling and improved cardiac function. Mechanistically, we show that HG induces extracellular advanced glycation end products (AGEs), which bind directly to MD2, leading to formation of AGEs-MD2-TLR4 complex and initiation of pro-inflammatory pathways. We further detect elevated AGE-MD2 complexes in heart tissues and serum of diabetic mice and human subjects with DCM. In summary, we uncover a new mechanism of HG-induced inflammatory responses and myocardial injury, in which AGE products directly bind MD2 to drive inflammatory DCM. The mechanisms underlying cardiac inflammation in diabetic cardiomyopathy are incompletely understood. Here the authors show that advanced glycation end products bind to the TLR4 co-receptor MD2 initiating pro-inflammatory pathways.