Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells

Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When u...

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Published inScientific reports Vol. 11; no. 1; pp. 7756 - 8
Main Authors Penedo, Marcos, Shirokawa, Tetsuya, Alam, Mohammad Shahidul, Miyazawa, Keisuke, Ichikawa, Takehiko, Okano, Naoko, Furusho, Hirotoshi, Nakamura, Chikashi, Fukuma, Takeshi
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 08.04.2021
Nature Publishing Group
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631/57/2282
639/301/930/328/1262
639/301/930/328/968
639/925/350/1056
639/925/350/2093
639/925/350/59
639/925/930/328/1262
639/925/930/328/968
Atomic force microscopy
Biosensors
Cell membranes
Computer engineering
Drug delivery
Drug delivery systems
Efficiency
Experiments
Humanities and Social Sciences
Life sciences
Membranes
Microscopes
Microscopy
multidisciplinary
Science
Science (multidisciplinary)
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Abstract Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.
AbstractList Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.
Abstract Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.
Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.
ArticleNumber 7756
Author Alam, Mohammad Shahidul
Miyazawa, Keisuke
Shirokawa, Tetsuya
Ichikawa, Takehiko
Okano, Naoko
Furusho, Hirotoshi
Penedo, Marcos
Fukuma, Takeshi
Nakamura, Chikashi
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Snippet Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery,...
Abstract Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug...
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Atomic force microscopy
Biosensors
Cell membranes
Computer engineering
Drug delivery
Drug delivery systems
Efficiency
Experiments
Humanities and Social Sciences
Life sciences
Membranes
Microscopes
Microscopy
multidisciplinary
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Science (multidisciplinary)
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Title Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells
URI https://link.springer.com/article/10.1038/s41598-021-87319-3
https://www.ncbi.nlm.nih.gov/pubmed/33833307
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https://www.proquest.com/docview/2511239686
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Volume 11
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