3D Bayesian cluster analysis of super-resolution data reveals LAT recruitment to the T cell synapse

• Published in: Scientific reports Vol. 7; no. 1; pp. 4077 - 9
• Main Authors: Griffié, Juliette, Shlomovich, Leigh, Williamson, David J., Shannon, Michael, Aaron, Jesse, Khuon, Satya, L. Burn, Garth, Boelen, Lies, Peters, Ruby, Cope, Andrew P., Cohen, Edward A. K., Rubin-Delanchy, Patrick, Owen, Dylan M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.06.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 14/35; 14/63; 631/1647/328/2238; 631/250/2503; 631/57/2265; Adaptor Proteins, Signal Transducing - metabolism; Bayes Theorem; Bayesian analysis; Cell Line; Cluster Analysis; Data processing; Fluorophores; Humanities and Social Sciences; Humans; Immunological synapses; Immunological Synapses - metabolism; Localization; Lymphocyte Activation - immunology; Lymphocytes T; Membrane Proteins - metabolism; Microscopy; Models, Biological; multidisciplinary; Science; Science (multidisciplinary); Synapses; T-Lymphocytes - immunology; T-Lymphocytes - metabolism

Single-molecule localisation microscopy (SMLM) allows the localisation of fluorophores with a precision of 10–30 nm, revealing the cell’s nanoscale architecture at the molecular level. Recently, SMLM has been extended to 3D, providing a unique insight into cellular machinery. Although cluster analys...