Maintenance of Nucleolar Homeostasis by CBX4 Alleviates Senescence and Osteoarthritis

• Published in: Cell reports (Cambridge) Vol. 26; no. 13; pp. 3643 - 3656.e7
• Main Authors: Ren, Xiaoqing, Hu, Boqiang, Song, Moshi, Ding, Zhichao, Dang, Yujiao, Liu, Zunpeng, Zhang, Weiqi, Ji, Qianzhao, Ren, Ruotong, Ding, Jianjian, Chan, Piu, Jiang, Changtao, Ye, Keqiong, Qu, Jing, Tang, Fuchou, Liu, Guang-Hui
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.03.2019; Elsevier
• Subjects: aging; Animals; CBX4; Cell Nucleolus - physiology; Cellular Senescence - physiology; Chromosomal Proteins, Non-Histone - metabolism; CRISPR/Cas9; epigenetics; gene editing; Gene Knockout Techniques; Genetic Therapy; HEK293 Cells; heterochromatin; Homeostasis; Humans; Ligases - genetics; Ligases - physiology; Male; Mesenchymal Stem Cells - physiology; Mice, Inbred C57BL; Mice, Inbred NOD; nucleolus; osteoarthritis; Osteoarthritis - therapy; Polycomb-Group Proteins - genetics; Polycomb-Group Proteins - physiology; rDNA; stem cell; rDNA; CBX4; stem cell; CRISPR/Cas9; osteoarthritis; nucleolus; aging; gene editing; epigenetics; heterochromatin

CBX4, a component of polycomb repressive complex 1 (PRC1), plays important roles in the maintenance of cell identity and organ development through gene silencing. However, whether CBX4 regulates human stem cell homeostasis remains unclear. Here, we demonstrate that CBX4 counteracts human mesenchymal...