TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production

The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differ...

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Published inScientific reports Vol. 7; no. 1; pp. 6601 - 11
Main Authors Hau, Kwan-Leong, Ranzoni, Anna Maria, Vlahova, Filipa, Hawkins, Kate, De Coppi, Paolo, David, Anna L., Guillot, Pascale V.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 26.07.2017
Nature Publishing Group
Nature Portfolio
Subjects
13/100
13/106
13/31
14/63
38/39
5'-Nucleotidase - metabolism
631/532
631/532/1360
Adenosine
Adenosine - metabolism
Amniotic fluid
Amniotic Fluid - cytology
CD73 antigen
Cell culture
Cell Differentiation - drug effects
Cell proliferation
Cell Proliferation - drug effects
Embryos
GPI-Linked Proteins - metabolism
Humanities and Social Sciences
Humans
Mesenchyme
multidisciplinary
Osteogenesis
Science
Science (multidisciplinary)
Stem cells
Stem Cells - drug effects
Therapeutic applications
Transforming Growth Factor beta - metabolism
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Abstract The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells. We show that this occurs via TGFβ-induced activation of CD73 and the associated increase in the generation of extracellular adenosine. Our data demonstrate that culture conditions are decisive for the expansion of hAFSCs and that TGFβ present in ET conditions causes the phenotype of RS-hAFSCs to revert to an earlier state of stemness. Cultivating RS-hAFSCs in ET conditions with TGFβ may therefore increase their therapeutic potential for clinical applications.
AbstractList The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells. We show that this occurs via TGFβ-induced activation of CD73 and the associated increase in the generation of extracellular adenosine. Our data demonstrate that culture conditions are decisive for the expansion of hAFSCs and that TGFβ present in ET conditions causes the phenotype of RS-hAFSCs to revert to an earlier state of stemness. Cultivating RS-hAFSCs in ET conditions with TGFβ may therefore increase their therapeutic potential for clinical applications.
Abstract The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells. We show that this occurs via TGFβ-induced activation of CD73 and the associated increase in the generation of extracellular adenosine. Our data demonstrate that culture conditions are decisive for the expansion of hAFSCs and that TGFβ present in ET conditions causes the phenotype of RS-hAFSCs to revert to an earlier state of stemness. Cultivating RS-hAFSCs in ET conditions with TGFβ may therefore increase their therapeutic potential for clinical applications.
Abstract The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells. We show that this occurs via TGFβ-induced activation of CD73 and the associated increase in the generation of extracellular adenosine. Our data demonstrate that culture conditions are decisive for the expansion of hAFSCs and that TGFβ present in ET conditions causes the phenotype of RS-hAFSCs to revert to an earlier state of stemness. Cultivating RS-hAFSCs in ET conditions with TGFβ may therefore increase their therapeutic potential for clinical applications.
ArticleNumber 6601
Author Hau, Kwan-Leong
Guillot, Pascale V.
Ranzoni, Anna Maria
Vlahova, Filipa
David, Anna L.
Hawkins, Kate
De Coppi, Paolo
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Snippet The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and...
Abstract The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells...
Abstract The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells...
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StartPage 6601
SubjectTerms 13/100
13/106
13/31
14/63
38/39
5'-Nucleotidase - metabolism
631/532
631/532/1360
Adenosine
Adenosine - metabolism
Amniotic fluid
Amniotic Fluid - cytology
CD73 antigen
Cell culture
Cell Differentiation - drug effects
Cell proliferation
Cell Proliferation - drug effects
Embryos
GPI-Linked Proteins - metabolism
Humanities and Social Sciences
Humans
Mesenchyme
multidisciplinary
Osteogenesis
Science
Science (multidisciplinary)
Stem cells
Stem Cells - drug effects
Therapeutic applications
Transforming Growth Factor beta - metabolism
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Title TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production
URI https://link.springer.com/article/10.1038/s41598-017-06780-1
https://www.ncbi.nlm.nih.gov/pubmed/28747757
https://www.proquest.com/docview/1956173090
https://search.proquest.com/docview/1924604511
https://pubmed.ncbi.nlm.nih.gov/PMC5529586
https://doaj.org/article/59a13a03c1544427a4059428931264d4
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