A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paper-based enzyme-linked immunosorbent assay

• Published in: Microsystems & nanoengineering Vol. 8; no. 1; pp. 53 - 10
• Main Authors: Lin, Dong, Li, Bowei, Fu, Longwen, Qi, Ji, Xia, Chunlei, Zhang, Yi, Chen, Jiadong, Choo, Jaebum, Chen, Lingxin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19.05.2022; Springer Nature B.V; Nature Publishing Group
• Subjects: 639/301; 639/638; Antigens; Biomarkers; Carcinoembryonic antigen; Cellulose esters; Cellulose nitrate; Diagnosis; Engineering; Enzyme-linked immunosorbent assay; Enzymes; Hydrophobicity; Immunoassay; Membranes; Microfluidics; Multiplexing; Polyurethane; Polyurethane resins; Remote regions; Screen printing; Substrates; Ultraviolet radiation; Materials science; Chemistry

Nitrocellulose (NC) membranes, as porous paper-like substrates with high protein-binding capabilities, are very popular in the field of point-of-care immunoassays. However, generating robust hydrophobic structures in NC membranes to fabricate microfluidic paper-based analytical devices (μPADs) remai...