Real-time multiplex PCR for simultaneous detection of multiple species from environmental DNA: an application on two Japanese medaka species

• Published in: Scientific reports Vol. 8; no. 1; pp. 9138 - 8
• Main Authors: Tsuji, Satsuki, Iguchi, Yuka, Shibata, Naoki, Teramura, Iori, Kitagawa, Tadao, Yamanaka, Hiroki
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.06.2018; Nature Publishing Group
• Subjects: 45/77; 631/158/2452; 631/158/2459; Aquariums; Aquatic organisms; Deoxyribonucleic acid; DNA; Ecological studies; Environmental DNA; Humanities and Social Sciences; multidisciplinary; Oryzias latipes; Polymerase chain reaction; Science; Science (multidisciplinary); Species

Information about species distribution is crucial to ecological studies. Environmental DNA (eDNA) analysis has recently been used to estimate the distribution of aquatic organisms. Several analytical methods including metabarcoding and species-specific PCR are being used for eDNA analysis. However,...