A toolbox for imaging RIPK1, RIPK3, and MLKL in mouse and human cells

• Published in: Cell death and differentiation Vol. 28; no. 7; pp. 2126 - 2144
• Main Authors: Samson, André L., Fitzgibbon, Cheree, Patel, Komal M., Hildebrand, Joanne M., Whitehead, Lachlan W., Rimes, Joel S., Jacobsen, Annette V., Horne, Christopher R., Gavin, Xavier J., Young, Samuel N., Rogers, Kelly L., Hawkins, Edwin D., Murphy, James M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.07.2021; Nature Publishing Group
• Subjects: 13; 14; 14/1; 14/19; 14/63; 38; 631/80; 631/92/275; 82; 82/16; 96; 96/95; Animals; Antibodies; Apoptosis; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell Cycle Analysis; Cell death; Cell Membrane - metabolism; Cytoplasm; HT29 Cells; Humans; Immunofluorescence; Inflammation; Life Sciences; Lipid bilayers; Mice; Microscopy; Necroptosis; Oligomerization; Phosphorylation; Protein Kinases - metabolism; Receptor-Interacting Protein Serine-Threonine Kinases - metabolism; Stem Cells

Necroptosis is a lytic, inflammatory cell death pathway that is dysregulated in many human pathologies. The pathway is executed by a core machinery comprising the RIPK1 and RIPK3 kinases, which assemble into necrosomes in the cytoplasm, and the terminal effector pseudokinase, MLKL. RIPK3-mediated ph...