Single-cell transcriptomic analysis of bloodstream Trypanosoma brucei reconstructs cell cycle progression and developmental quorum sensing

• Published in: Nature communications Vol. 12; no. 1; p. 5268
• Main Authors: Briggs, Emma M., Rojas, Federico, McCulloch, Richard, Matthews, Keith R., Otto, Thomas D.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.09.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 14/1; 14/63; 38/39; 38/62; 38/90; 38/91; 631/114; 631/136/142; 631/326/417; Animals; Cell cycle; Cell Cycle - genetics; Cell Cycle - physiology; Differentiation; Failure analysis; Gene expression; Gene Expression Regulation; Humanities and Social Sciences; multidisciplinary; Mutation; Parasites; Protozoan Proteins - genetics; Quorum Sensing; Science; Science (multidisciplinary); Sequence Analysis, RNA - methods; Single-Cell Analysis; Transcriptomes; Trypanosoma brucei; Trypanosoma brucei brucei - cytology; Trypanosoma brucei brucei - genetics; Trypanosoma brucei brucei - physiology; Trypanosome

Developmental steps in the trypanosome life-cycle involve transition between replicative and non-replicative forms specialised for survival in, and transmission between, mammalian and tsetse fly hosts. Here, using oligopeptide-induced differentiation in vitro, we model the progressive development of...