Interferometric rotating point spread function

Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localization-based super-resolution imaging because of their large working depth range. In this article, we propose an interferometric DH PSF (iDH PSF) using two opposed objective lenses as in the 4Pi micros...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 7; no. 1; pp. 5882 - 7
Main Authors Wang, Wei, Situ, Guohai
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 19.07.2017
Nature Publishing Group
Nature Portfolio
Subjects
14/63
639/624/1107/328/2238
639/624/1107/510
Humanities and Social Sciences
Localization
multidisciplinary
Science
Science (multidisciplinary)
Online AccessGet full text

Cover

More Information
Summary:Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localization-based super-resolution imaging because of their large working depth range. In this article, we propose an interferometric DH PSF (iDH PSF) using two opposed objective lenses as in the 4Pi microscope. In the proposed iDH PSF, the super-resolution in the axial PSF is transferred to the azimuthal rotation. Moreover, we design an iDH PSF whose imaging range reaches 3  μm , which is roughly 3 times as much as that which can be obtained by using other interferometric localization-based super-resolution imaging methods.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-06203-1