AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2

• Published in: Nature communications Vol. 11; no. 1; pp. 2765 - 13
• Main Authors: Yang, Acong, Shao, Tie-Juan, Bofill-De Ros, Xavier, Lian, Chuanjiang, Villanueva, Patricia, Dai, Lisheng, Gu, Shuo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.06.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 38/1; 38/39; 42/109; 42/70; 45/29; 45/71; 45/77; 45/88; 45/91; 49/90; 631/337/1645; 631/337/384/331; 631/45/500; Argonaute Proteins - genetics; Argonaute Proteins - metabolism; Cancer; Decay; Degradation; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Exonuclease; Exoribonucleases - genetics; Exoribonucleases - metabolism; Gene expression; Gene Knockout Techniques; HEK293 Cells; High-Throughput Nucleotide Sequencing; Humanities and Social Sciences; Humans; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; multidisciplinary; Mutation; Proteins; Ribonucleic acid; RNA; RNA Nucleotidyltransferases - genetics; RNA Nucleotidyltransferases - metabolism; Science; Science (multidisciplinary)

MicroRNAs (miRNAs) associated with Argonaute proteins (AGOs) regulate gene expression in mammals. miRNA 3’ ends are subject to frequent sequence modifications, which have been proposed to affect miRNA stability. However, the underlying mechanism is not well understood. Here, by genetic and biochemic...