Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules

• Published in: Nature communications Vol. 13; no. 1; p. 4704
• Main Authors: Castrogiovanni, Cédric, Inchingolo, Alessio V., Harrison, Jonathan U., Dudka, Damian, Sen, Onur, Burroughs, Nigel J., McAinsh, Andrew D., Meraldi, Patrick
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 10.08.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 13/109; 13/51; 14/19; 14/35; 14/63; 631/1647/328; 631/80/128/1653; 631/80/641/1656; 82/80; Antibodies; Binding; Binding sites; Cell division; Chromosomes; Fibers; Guanosine diphosphate; Guanosine triphosphate; Guanosine Triphosphate - metabolism; Humanities and Social Sciences; Kinetochores; Kinetochores - metabolism; Lattices; Localization; Microtubule-Associated Proteins - metabolism; Microtubules; Microtubules - metabolism; Mitosis; multidisciplinary; Nucleotides; Nucleotides - metabolism; Proteins; Science; Science (multidisciplinary); Spindle Apparatus - metabolism; Tubulin; Tubulin - metabolism

Current models infer that the microtubule-based mitotic spindle is built from GDP-tubulin with small GTP caps at microtubule plus-ends, including those that attach to kinetochores, forming the kinetochore-fibres. Here we reveal that kinetochore-fibres additionally contain a dynamic mixed-nucleotide...