Highly efficient RNA-guided base editing in rabbit

• Published in: Nature communications Vol. 9; no. 1; pp. 2717 - 10
• Main Authors: Liu, Zhiquan, Chen, Mao, Chen, Siyu, Deng, Jichao, Song, Yuning, Lai, Liangxue, Li, Zhanjun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.07.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 38/23; 42/41; 631/208/4041; 631/208/4041/3196; Adenine; Adenine - metabolism; Adenine deaminase; Adenosine Deaminase - genetics; Adenosine Deaminase - metabolism; Animal models; Animals; Base Pairing; Base Sequence; Blastocyst - cytology; Blastocyst - metabolism; Blastocysts; Codon, Nonsense; CRISPR-Associated Protein 9 - genetics; CRISPR-Associated Protein 9 - metabolism; CRISPR-Cas Systems; Cytidine - metabolism; Cytidine deaminase; Cytidine Deaminase - genetics; Cytidine Deaminase - metabolism; Disease Models, Animal; Founder Effect; Gene Editing - methods; Humanities and Social Sciences; Humans; INDEL Mutation; Missense mutation; multidisciplinary; Mutation; Mutation, Missense; Rabbits; Rabbits - genetics; Ribonucleic acid; RNA; RNA editing; RNA, Guide, CRISPR-Cas Systems; Science; Science (multidisciplinary); Sequence Alignment; Splicing

Cytidine base editors (CBEs) and adenine base editors (ABEs), composed of a cytidine deaminase or an evolved adenine deaminase fused to Cas9 nickase, enable the conversion of C·G to T·A or A·T to G·C base pair in organisms, respectively. Here, we show that BE3 and ABE7.10 systems can achieve a targe...