Molecular cloning of kman coding for mannanase from Klebsiella oxytoca KUB-CW2-3 and its hybrid mannanase characters

• Published in: Enzyme and microbial technology Vol. 89; pp. 39 - 51
• Main Authors: Pongsapipatana, Nawapan, Damrongteerapap, Piyanat, Chantorn, Sudathip, Sintuprapa, Wilawan, Keawsompong, Suttipun, Nitisinprasert, Sunee
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2016
• Subjects: adverse effects; amino acid composition; Amino Acid Sequence; amino acid sequences; amino acids; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; beta-mannosidase; beta-Mannosidase - chemistry; beta-Mannosidase - genetics; beta-Mannosidase - metabolism; carboxymethylcellulose; Characters; Cloning, Molecular; coconut meal; digestion; DNA fragmentation; E. coli; Escherichia coli; Escherichia coli - enzymology; Escherichia coli - genetics; genes; Genes, Bacterial; hydrolysates; Klebsiella oxytoca; Klebsiella oxytoca - enzymology; Klebsiella oxytoca - genetics; konjac mannan; lactic acid bacteria; Lactobacillus reuteri; locust bean gum; Mannans - metabolism; Models, Molecular; Molecular cloning; molecular weight; oligosaccharides; open reading frames; pathogens; Protein Conformation; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; specific growth rate; Substrate Specificity; temperature; Weissella confusa; β-mannanase; β-mannanase; Molecular cloning; E. coli; Characters

•Mannanase gene from K. oxytoca KUB-CW2-3 was cloned into pFlag-CTS by E.coli system.•The clone KMAN-3 showed higher mannanase activity than the wild type for 400 times.•Wide substrate specificity and high productivity indicated its novel mannanase source.•Its hydrolysates enhancing beneficial bacte...