RNA-Seq of human whole blood: Evaluation of globin RNA depletion on Ribo-Zero library method

• Published in: Scientific reports Vol. 10; no. 1; p. 6271
• Main Authors: Harrington, Christina A., Fei, Suzanne S., Minnier, Jessica, Carbone, Lucia, Searles, Robert, Davis, Brett A., Ogle, Kimberly, Planck, Stephen R., Rosenbaum, James T., Choi, Dongseok
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.04.2020; Nature Publishing Group
• Subjects: 38; 38/91; 45; 631/1647/2017; 631/337/2019; 692/308/53; Blood; Gene expression; Globins - genetics; Hemoglobin; Humanities and Social Sciences; Humans; Libraries; multidisciplinary; Peripheral blood; RNA - analysis; RNA-Seq; rRNA; Science; Science (multidisciplinary); Specimen Handling - methods; Statistical analysis; Transcriptome

Peripheral blood is a highly accessible biofluid providing a rich source of information about human physiology and health status. However, for studies of the blood transcriptome with RNA sequencing (RNA-Seq) techniques, high levels of hemoglobin mRNAs (hgbRNA) present in blood can occupy valuable se...