Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum

• Published in: Communications biology Vol. 5; no. 1; p. 344
• Main Authors: Che, Ping, Wu, Emily, Simon, Marissa K., Anand, Ajith, Lowe, Keith, Gao, Huirong, Sigmund, Amy L., Yang, Meizhu, Albertsen, Marc C., Gordon-Kamm, William, Jones, Todd J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11.04.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/106; 42; 42/41; 631/449; 631/61; Agrobacterium; Biology; Biomedical and Life Sciences; Callus; Cell culture; CRISPR; CRISPR-Cas Systems; Edible Grain - genetics; Gene Editing - methods; Genetic transformation; Genomes; Genotypes; Life Sciences; Plants, Genetically Modified - genetics; Regeneration - genetics; Sorghum; Sorghum - genetics; Sorghum bicolor; Tissue culture

For many important crops including sorghum, use of CRISPR/Cas technology is limited not only by the delivery of the gene-modification components into a plant cell, but also by the ability to regenerate a fertile plant from the engineered cell through tissue culture. Here, we report that Wuschel2 (Wu...