A deep learning-driven low-power, accurate, and portable platform for rapid detection of COVID-19 using reverse-transcription loop-mediated isothermal amplification

• Published in: Scientific reports Vol. 12; no. 1; pp. 4132 - 18
• Main Authors: Waheed, Waqas, Saylan, Sueda, Hassan, Taimur, Kannout, Hussain, Alsafar, Habiba, Alazzam, Anas
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.03.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/2017/2003; 692/699/255; Area Under Curve; Coloring Agents - chemistry; Coronaviruses; COVID-19; COVID-19 - diagnosis; COVID-19 Testing; Deep Learning; Dyes; Evaporation; Gene amplification; Humanities and Social Sciences; Humans; Molecular Diagnostic Techniques - instrumentation; Molecular Diagnostic Techniques - methods; multidisciplinary; Nasopharynx - virology; Nucleic Acid Amplification Techniques - instrumentation; Nucleic Acid Amplification Techniques - methods; pH effects; Point-of-Care Systems; Printing, Three-Dimensional; RNA, Viral - analysis; RNA, Viral - metabolism; ROC Curve; Sample preparation; SARS-CoV-2 - genetics; SARS-CoV-2 - isolation & purification; Science; Science (multidisciplinary); Sensitivity and Specificity
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-07954-2

This paper presents a deep learning-driven portable, accurate, low-cost, and easy-to-use device to perform Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) to facilitate rapid detection of COVID-19. The 3D-printed device—powered using only a 5 Volt AC-DC adapter—can perform 16...