A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies

• Published in: Nature communications Vol. 12; no. 1; pp. 1722 - 12
• Main Authors: Creanga, Adrian, Gillespie, Rebecca A., Fisher, Brian E., Andrews, Sarah F., Lederhofer, Julia, Yap, Christina, Hatch, Liam, Stephens, Tyler, Tsybovsky, Yaroslav, Crank, Michelle C., Ledgerwood, Julie E., McDermott, Adrian B., Mascola, John R., Graham, Barney S., Kanekiyo, Masaru
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19.03.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13/1; 13/106; 14/35; 14/63; 49/47; 631/1647/2234; 631/1647/664; 631/326/596/1578; Antibodies; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Antibody response; Antigens, Viral - immunology; Biosafety; Containment; Depth profiling; Gene Expression Profiling; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Hemagglutinins; Humanities and Social Sciences; Humans; Immunity; Influenza; Influenza A; Influenza A virus - genetics; Influenza A virus - immunology; Influenza A Virus, H1N1 Subtype - immunology; Influenza A Virus, H3N2 Subtype - immunology; Influenza Vaccines - immunology; Influenza, Human - virology; multidisciplinary; Neutralization; Neutralizing; Orthomyxoviridae Infections - virology; Pandemics; Phylogeny; Replication; Reporter gene; Science; Science (multidisciplinary); Vaccine development; Vaccines; Viruses

Broadly neutralizing antibodies (bnAbs) have been developed as potential countermeasures for seasonal and pandemic influenza. Deep characterization of these bnAbs and polyclonal sera provides pivotal understanding for influenza immunity and informs effective vaccine design. However, conventional vir...