Evaluation of biological effects of intermediate frequency magnetic field on differentiation of embryonic stem cell

The embryotoxic effect of intermediate frequency (IF) magnetic field (MF) was evaluated using murine embryonic stem (ES) cells and fibroblast cells based on the embryonic stem cell test (EST). The cells were exposed to 21 kHz IF-MF up to magnetic flux density of 3.9 mT during the cell proliferation...

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Published inToxicology reports Vol. 3; pp. 135 - 140
Main Authors Yoshie, Sachiko, Ogasawara, Yuki, Ikehata, Masateru, Ishii, Kazuyuki, Suzuki, Yukihisa, Wada, Keiji, Wake, Kanako, Nakasono, Satoshi, Taki, Masao, Ohkubo, Chiyoji
Format Journal Article
LanguageEnglish
Published Ireland Elsevier 01.01.2016
Subjects
MF, magnetic field
EST, embryonic stem cell test
Intermediate frequency magnetic field
Embryonic stem cell
EB, embryonic body
EMF, electromagnetic field
Gene expression
IF, intermediate frequency
ELF, extremely low frequency
ES, embryonic stem
5-FU, 5-fluorouracil
ICNIRP, International Commission of Non-Ionizing Radiation Protection
RF, radiofrequency
Differentiation
WHO, World Health Organization
IH, induction heating
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Abstract The embryotoxic effect of intermediate frequency (IF) magnetic field (MF) was evaluated using murine embryonic stem (ES) cells and fibroblast cells based on the embryonic stem cell test (EST). The cells were exposed to 21 kHz IF-MF up to magnetic flux density of 3.9 mT during the cell proliferation process (7 days) or the cell differentiation process (10 days) during which an embryonic body differentiated into myocardial cells. As a result, there was no significant difference in the cell proliferation between sham- and IF-MF-exposed cells for both ES and fibroblast cells. Similarly, the ratio of the number of ES-derived cell aggregates differentiated to myocardial cells to total number of cell aggregates was not changed by IF-MF exposure. In addition, the expressions of a cardiomyocytes-specific gene, , and an early developmental gene, , in the exposed cell aggregate were not altered. Since the magnetic flux density adopted in this study is much higher than that generated by an inverter of the electrical railway, an induction heating (IH) cooktop, . in our daily lives, these results suggested that IF-MF in which the public is exposed to in general living environment would not have embryotoxic effect.
AbstractList The embryotoxic effect of intermediate frequency (IF) magnetic field (MF) was evaluated using murine embryonic stem (ES) cells and fibroblast cells based on the embryonic stem cell test (EST). The cells were exposed to 21 kHz IF–MF up to magnetic flux density of 3.9 mT during the cell proliferation process (7 days) or the cell differentiation process (10 days) during which an embryonic body differentiated into myocardial cells. As a result, there was no significant difference in the cell proliferation between sham- and IF–MF-exposed cells for both ES and fibroblast cells. Similarly, the ratio of the number of ES-derived cell aggregates differentiated to myocardial cells to total number of cell aggregates was not changed by IF–MF exposure. In addition, the expressions of a cardiomyocytes-specific gene, Myl2, and an early developmental gene, Hba-x, in the exposed cell aggregate were not altered. Since the magnetic flux density adopted in this study is much higher than that generated by an inverter of the electrical railway, an induction heating (IH) cooktop, etc. in our daily lives, these results suggested that IF–MF in which the public is exposed to in general living environment would not have embryotoxic effect. Keywords: Differentiation, Embryonic stem cell, Gene expression, Intermediate frequency magnetic field
The embryotoxic effect of intermediate frequency (IF) magnetic field (MF) was evaluated using murine embryonic stem (ES) cells and fibroblast cells based on the embryonic stem cell test (EST). The cells were exposed to 21 kHz IF–MF up to magnetic flux density of 3.9 mT during the cell proliferation process (7 days) or the cell differentiation process (10 days) during which an embryonic body differentiated into myocardial cells. As a result, there was no significant difference in the cell proliferation between sham- and IF–MF-exposed cells for both ES and fibroblast cells. Similarly, the ratio of the number of ES-derived cell aggregates differentiated to myocardial cells to total number of cell aggregates was not changed by IF–MF exposure. In addition, the expressions of a cardiomyocytes-specific gene, Myl2 , and an early developmental gene, Hba-x , in the exposed cell aggregate were not altered. Since the magnetic flux density adopted in this study is much higher than that generated by an inverter of the electrical railway, an induction heating (IH) cooktop, etc . in our daily lives, these results suggested that IF–MF in which the public is exposed to in general living environment would not have embryotoxic effect.
The embryotoxic effect of intermediate frequency (IF) magnetic field (MF) was evaluated using murine embryonic stem (ES) cells and fibroblast cells based on the embryonic stem cell test (EST). The cells were exposed to 21 kHz IF-MF up to magnetic flux density of 3.9 mT during the cell proliferation process (7 days) or the cell differentiation process (10 days) during which an embryonic body differentiated into myocardial cells. As a result, there was no significant difference in the cell proliferation between sham- and IF-MF-exposed cells for both ES and fibroblast cells. Similarly, the ratio of the number of ES-derived cell aggregates differentiated to myocardial cells to total number of cell aggregates was not changed by IF-MF exposure. In addition, the expressions of a cardiomyocytes-specific gene, , and an early developmental gene, , in the exposed cell aggregate were not altered. Since the magnetic flux density adopted in this study is much higher than that generated by an inverter of the electrical railway, an induction heating (IH) cooktop, . in our daily lives, these results suggested that IF-MF in which the public is exposed to in general living environment would not have embryotoxic effect.
Author Taki, Masao
Wada, Keiji
Yoshie, Sachiko
Suzuki, Yukihisa
Ogasawara, Yuki
Wake, Kanako
Ikehata, Masateru
Nakasono, Satoshi
Ishii, Kazuyuki
Ohkubo, Chiyoji
AuthorAffiliation f Japan EMF Information Center, 2-9-11 Shiba, Minato-ku, Tokyo 105-0014, Japan
a Biotechnology Laboratory, Human Science Division, Railway Technical Research Institute, 2-8-38, Hikari-cho, Kokubunji, Tokyo 185-8540, Japan
e EMF Environment Sector, Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko-shi, Chiba 270-1194, Japan
c Faculty of Electrical & Electronic Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0397, Japan
d Electromagnetic Compatibility Laboratory, Applied Electromagnetic Research Institute, National Institute of Information and Communications Technology, 4-2-1, Nukui-Kitamachi, Koganei, Tokyo 184-8795, Japan
b Hygienic Chemistry, Health and Environmental Sciences, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose-shi, Tokyo 204-8588, Japan
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  surname: Ohkubo
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  organization: Japan EMF Information Center, 2-9-11 Shiba, Minato-ku, Tokyo 105-0014, Japan
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Keywords MF, magnetic field
EST, embryonic stem cell test
Intermediate frequency magnetic field
Embryonic stem cell
EB, embryonic body
EMF, electromagnetic field
Gene expression
IF, intermediate frequency
ELF, extremely low frequency
ES, embryonic stem
5-FU, 5-fluorouracil
ICNIRP, International Commission of Non-Ionizing Radiation Protection
RF, radiofrequency
Differentiation
WHO, World Health Organization
IH, induction heating
Language English
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crossref_primary_10_1016_j_toxrep_2015_12_012
pubmed_primary_28959531
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PublicationDate 2016-01-01
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  year: 2016
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PublicationDecade 2010
PublicationPlace Ireland
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PublicationTitle Toxicology reports
PublicationTitleAlternate Toxicol Rep
PublicationYear 2016
Publisher Elsevier
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Snippet The embryotoxic effect of intermediate frequency (IF) magnetic field (MF) was evaluated using murine embryonic stem (ES) cells and fibroblast cells based on...
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Title Evaluation of biological effects of intermediate frequency magnetic field on differentiation of embryonic stem cell
URI https://www.ncbi.nlm.nih.gov/pubmed/28959531
https://pubmed.ncbi.nlm.nih.gov/PMC5615788
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