Integrative analysis of multi-platform reverse-phase protein array data for the pharmacodynamic assessment of response to targeted therapies

• Published in: Scientific reports Vol. 10; no. 1; p. 21985
• Main Authors: Byron, Adam, Bernhardt, Stephan, Ouine, Bérèngere, Cartier, Aurélie, Macleod, Kenneth G., Carragher, Neil O., Sibut, Vonick, Korf, Ulrike, Serrels, Bryan, de Koning, Leanne
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.12.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114/2401; 631/1647; 631/1647/2067; 631/67; 631/67/1857; 692/53; Antibodies; Antigens; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Breast cancer; Breast Neoplasms - drug therapy; Breast Neoplasms - pathology; Cancer; Cell Line, Tumor; Drug development; Female; Humanities and Social Sciences; Humans; Molecular Targeted Therapy; multidisciplinary; Pharmacodynamics; Phosphorylation; Post-translation; Principal Component Analysis; Protein Array Analysis - methods; Protein arrays; Proteins; Science; Science (multidisciplinary); Tumor cell lines

Reverse-phase protein array (RPPA) technology uses panels of high-specificity antibodies to measure proteins and protein post-translational modifications in cells and tissues. The approach offers sensitive and precise quantification of large numbers of samples and has thus found applications in the...