CRISPR/Cas9 small promoter deletion in H19 lncRNA is associated with altered cell morphology and proliferation

• Published in: Scientific reports Vol. 11; no. 1; pp. 18380 - 10
• Main Authors: da Silva Santos, Renan, Pinheiro, Daniel Pascoalino, Teixeira, Louhanna Pinheiro Rodrigues, Sales, Sarah Leyenne Alves, dos Santos Luciano, Maria Claudia, de Lima Melo, Mayara Magna, Pinheiro, Ronald Feitosa, Tavares, Kaio César Simiano, Furtado, Gilvan Pessoa, Pessoa, Claudia, Furtado, Cristiana Libardi Miranda
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.09.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/67/68/2486; 631/80/304; Animals; Base Sequence; Binding sites; Biomarkers, Tumor; Carcinogenesis; Carcinogenesis - genetics; Cell Cycle - genetics; Cell morphology; Cell Proliferation - genetics; Cell size; Chromosome aberrations; CRISPR; CRISPR-Cas Systems; Cytogenetic Analysis; Cytology; DNA sequencing; Drug resistance; Gene deletion; Gene Editing; Gene expression; Gene Knockdown Techniques; Humanities and Social Sciences; Humans; Metastases; Mice; multidisciplinary; Myoblasts; Neoplasms - genetics; Neoplasms - pathology; Non-coding RNA; Promoter Regions, Genetic; RNA, Long Noncoding - chemistry; RNA, Long Noncoding - genetics; Science; Science (multidisciplinary); Sequence Deletion; Transcription; Tumor suppressor genes; Tumorigenesis

The imprinted H19 long non-coding RNA, a knowing oncofetal gene, presents a controversial role during the carcinogenesis process since its tumor suppressor or oncogenic activity is not completely elucidated. Since H19 lncRNA is involved in many biological pathways related to tumorigenesis, we sought...