An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae

• Published in: Nature communications Vol. 13; no. 1; pp. 2895 - 12
• Main Authors: Peng, Bingyin, Esquirol, Lygie, Lu, Zeyu, Shen, Qianyi, Cheah, Li Chen, Howard, Christopher B., Scott, Colin, Trau, Matt, Dumsday, Geoff, Vickers, Claudia E.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.05.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 38; 38/22; 38/35; 38/39; 38/77; 42/44; 45/23; 45/44; 631/1647/1511; 631/326/2522; 631/61/252/318; 82/80; Amplification; Dosage; Factories; Gene Amplification; Gene dosage; Gene expression; Genomes; Haploinsufficiency; Humanities and Social Sciences; In vivo methods and tests; Industrial engineering; Integration; Limonene; Limonene - metabolism; Lycopene; Manufacturing engineering; Metabolic engineering; Metabolic Engineering - methods; Metabolic pathways; Metabolism; Microorganisms; multidisciplinary; Nerolidol; Proteins; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Science; Science (multidisciplinary); Yeast; Yeasts

Bottlenecks in metabolic pathways due to insufficient gene expression levels remain a significant problem for industrial bioproduction using microbial cell factories. Increasing gene dosage can overcome these bottlenecks, but current approaches suffer from numerous drawbacks. Here, we describe HapAm...