Comparing DNA enrichment of proliferating cells following administration of different stable isotopes of heavy water

• Published in: Scientific reports Vol. 7; no. 1; pp. 4043 - 12
• Main Authors: Farthing, Don E., Buxbaum, Nataliya P., Lucas, Philip J., Maglakelidze, Natella, Oliver, Brittany, Wang, Jiun, Hu, Kevin, Castro, Ehydel, Bare, Catherine V., Gress, Ronald E.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.06.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 13/106; 13/31; 631/1647/2196/1379; 631/67/70; 631/92/147; 639/638/11/296; 64/60; 692/308/575; 82/16; 82/56; Animals; Body water; Bone marrow; Cell growth; Cell Line, Tumor; Cell Proliferation; Deoxyadenosine; Deoxyribonucleic acid; Deuterium; Deuterium Oxide - pharmacology; DNA; DNA - chemistry; DNA - genetics; DNA Replication - drug effects; Headspace; Heavy water; Humanities and Social Sciences; Ionization; Isotope Labeling; Isotopes; Kinases; Mass Spectrometry; Mice; multidisciplinary; Quantitation; Radioactive labeling; Science; Science (multidisciplinary); Stable isotopes; Thymus; Water levels

Deuterated water ( 2 H 2 O) is a label commonly used for safe quantitative measurement of deuterium enrichment into DNA of proliferating cells. More recently, it has been used for labeling proteins and other biomolecules. Our in vitro - in vivo research reports important stable isotopic labeling enr...