Identification of replication fork-associated proteins in Drosophila embryos and cultured cells using iPOND coupled to quantitative mass spectrometry

• Published in: Scientific reports Vol. 12; no. 1; pp. 6903 - 11
• Main Authors: Munden, Alexander, Wright, Madison T., Han, Dongsheng, Tirgar, Reyhaneh, Plate, Lars, Nordman, Jared T.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.04.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/136; 631/45; 631/45/147; 631/80/103; Animals; Cells, Cultured; Chromatin - genetics; Chromatin remodeling; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA repair; DNA Replication; Drosophila; Drosophila - genetics; Embryos; Epigenetics; Genomes; Genomic Instability; Humanities and Social Sciences; Insects; Mass spectrometry; Mass Spectrometry - methods; Mass spectroscopy; multidisciplinary; Proteins; Replication forks; Science; Science (multidisciplinary); Scientific imaging; Ubiquitin; Ubiquitin-protein ligase

Replication of the eukaryotic genome requires the formation of thousands of replication forks that must work in concert to accurately replicate the genetic and epigenetic information. Defining replication fork-associated proteins is a key step in understanding how genomes are replicated and repaired...