Novel episomal vectors and a highly efficient transformation procedure for the fission yeast Schizosaccharomyces japonicus

• Published in: Yeast (Chichester, England) Vol. 27; no. 12; pp. 1049 - 1060
• Main Authors: Aoki, Keita, Nakajima, Reiko, Furuya, Kanji, Niki, Hironori
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.12.2010
• Subjects: fission yeast; Genetic Techniques; Genetic Vectors - genetics; Molecular Sequence Data; plasmid; Plasmids - genetics; Schizosaccharomyces; Schizosaccharomyces - genetics; Schizosaccharomyces - growth & development; Schizosaccharomyces pombe; Sz. japonicus; transformation; Transformation, Genetic

Schizosaccharomyces japonicus is a fission yeast for which new genetic tools have recently been developed. Here, we report novel plasmid vectors with high transformation efficiency and an electroporation method for Sz. japonicus. We isolated 44 replicating segments from 12 166 transformants of Sz. j...