Rapid and robust squashed spore/colony PCR of industrially important fungi

• Published in: Fungal biology and biotechnology Vol. 10; no. 1; p. 15
• Main Authors: Yuan, Guoliang, Czajka, Jeffrey J., Dai, Ziyu, Hu, Dehong, Pomraning, Kyle R., Hofstad, Beth A., Kim, Joonhoon, Robles, Ana L., Deng, Shuang, Magnuson, Jon K.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.07.2023; BioMed Central; BMC
• Subjects: Analysis; BASIC BIOLOGICAL SCIENCES; Cell division; Colonies; Colony PCR; Deoxyribonucleic acid; Dilution; DNA; DNA polymerase; DNA-directed DNA polymerase; Enzymes; Fungi; Genetic engineering; Genetic testing; Genetically modified organisms; Genomics; Industrial applications; Metabolic engineering; Methodology; Methods; Microorganisms; Polymerase chain reaction; Quick screening; Robustness; Spore concentration; Spore PCR; Spores; Squash preparation; Synthetic biology; Yeast; Niger; Squash preparation; Spore PCR; Colony PCR; Spore concentration; Quick screening

Fungi have been utilized for centuries in medical, agricultural, and industrial applications. Development of systems biology techniques has enabled the design and metabolic engineering of these fungi to produce novel fuels, chemicals, and enzymes from renewable feedstocks. Many genetic tools have be...