RootScan: Software for high-throughput analysis of root anatomical traits

• Published in: Plant and soil Vol. 357; no. 1-2; pp. 189 - 203
• Main Authors: Burton, Amy L., Williams, Michael, Lynch, Jonathan P., Brown, Kathleen M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.08.2012; Springer Netherlands; Springer Nature B.V
• Subjects: Agronomy; Agronomy. Soil science and plant productions; Animal, plant and microbial ecology; Biological and medical sciences; Biomedical and Life Sciences; computer software; Corn; Correlation analysis; Cross-sections; Data collection; Ecology; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Genotype & phenotype; Genotypes; Image analysis; Image contrast; Life Sciences; Phenotypic traits; Pixels; Plant Physiology; Plant roots; Plant Sciences; Plants; Regular Article; Root stele; roots; Software; Soil Science & Conservation; Soil-plant relationships. Soil fertility; Soil-plant relationships. Soil fertility. Fertilization. Amendments; user interface; Xylem vessels; Maize; Aerenchyma; Trait; Root; Anatomy; Phenotyping; Plant tissue; Monocotyledones; Vegetals; Zea mays; Parenchyma; C4-Type; Cereal crop; Phenotype; Computer program; Gramineae; Analysis; Angiospermae; Vegetative apparatus; Spermatophyta; Software; Soil plant relation

Background and aims RootScan is a program for semi-automated image analysis of anatomical traits in root cross-sections. Methods RootScan uses pixel thresholds to separate the cross-section from its background and to divide it into tissue regions. Area measurements and object counts are performed wi...