Characterization and functional analysis of hybrid pearl gentian grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) complement C3 against Cryptocaryon irritans infection
•C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper.•The expression level of PGC3 was increased in infected grouper serum.•PGC3 covered on the surface of C. irritans trophont. Pearl gentian grouper is a new aquacultural hybrid resulted from breedi...
Saved in:
| Published in | Fish and shellfish immunology reports Vol. 2; p. 100032 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier Ltd
01.12.2021
Elsevier |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | •C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper.•The expression level of PGC3 was increased in infected grouper serum.•PGC3 covered on the surface of C. irritans trophont.
Pearl gentian grouper is a new aquacultural hybrid resulted from breeding of female tiger grouper (Epinephelus fuscogutattus) and male giant grouper (E. lanceolatus). Our preliminary study found that pearl gentian grouper exhibits less susceptible to the primary infection of Cryptocaryon irritans, which is an important parasitic ciliate in marine aquaculture, indicated that pearl gentian grouper might own a strong innate immune system. Complement system play key roles in innate immunity, whether pearl gentian grouper's complement component contribute for the defensing against the C. irritans infection remain unclear. In the present study, we found that C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper, suggested that the heat-labile components in serum are responsible for the immobilization of C. irritans. Moreover, we cloned and characterized the encoding sequence of pearl gentian grouper complement C3 (PGC3), a key component in complement system. We also found that the expression level of PGC3 was increased in infected grouper serum when compared with that of control grouper. Furthermore, the binding of PGC3 on the surface of C. irritans trophonts located on the grouper skin was detected. These data suggested that pearl gentian grouper's complement system indeed play roles in the immune response against the C. irritans infection. |
|---|---|
| AbstractList | •
C. irritans
can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper.
•
The expression level of PGC3 was increased in infected grouper serum.
•
PGC3 covered on the surface of
C. irritans
trophont.
Pearl gentian grouper is a new aquacultural hybrid resulted from breeding of female tiger grouper (
Epinephelus fuscogutattus
) and male giant grouper (
E. lanceolatus
). Our preliminary study found that pearl gentian grouper exhibits less susceptible to the primary infection of
Cryptocaryon irritans
, which is an important parasitic ciliate in marine aquaculture, indicated that pearl gentian grouper might own a strong innate immune system. Complement system play key roles in innate immunity, whether pearl gentian grouper's complement component contribute for the defensing against the
C. irritans
infection remain unclear. In the present study, we found that
C. irritans
can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper, suggested that the heat-labile components in serum are responsible for the immobilization of
C. irritans
. Moreover, we cloned and characterized the encoding sequence of pearl gentian grouper complement C3 (PGC3), a key component in complement system. We also found that the expression level of PGC3 was increased in infected grouper serum when compared with that of control grouper. Furthermore, the binding of PGC3 on the surface of
C. irritans
trophonts located on the grouper skin was detected. These data suggested that pearl gentian grouper's complement system indeed play roles in the immune response against the
C. irritans
infection. Pearl gentian grouper is a new aquacultural hybrid resulted from breeding of female tiger grouper (Epinephelus fuscogutattus) and male giant grouper (E. lanceolatus). Our preliminary study found that pearl gentian grouper exhibits less susceptible to the primary infection of Cryptocaryon irritans, which is an important parasitic ciliate in marine aquaculture, indicated that pearl gentian grouper might own a strong innate immune system. Complement system play key roles in innate immunity, whether pearl gentian grouper's complement component contribute for the defensing against the C. irritans infection remain unclear. In the present study, we found that C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper, suggested that the heat-labile components in serum are responsible for the immobilization of C. irritans. Moreover, we cloned and characterized the encoding sequence of pearl gentian grouper complement C3 (PGC3), a key component in complement system. We also found that the expression level of PGC3 was increased in infected grouper serum when compared with that of control grouper. Furthermore, the binding of PGC3 on the surface of C. irritans trophonts located on the grouper skin was detected. These data suggested that pearl gentian grouper's complement system indeed play roles in the immune response against the C. irritans infection. •C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper.•The expression level of PGC3 was increased in infected grouper serum.•PGC3 covered on the surface of C. irritans trophont. Pearl gentian grouper is a new aquacultural hybrid resulted from breeding of female tiger grouper (Epinephelus fuscogutattus) and male giant grouper (E. lanceolatus). Our preliminary study found that pearl gentian grouper exhibits less susceptible to the primary infection of Cryptocaryon irritans, which is an important parasitic ciliate in marine aquaculture, indicated that pearl gentian grouper might own a strong innate immune system. Complement system play key roles in innate immunity, whether pearl gentian grouper's complement component contribute for the defensing against the C. irritans infection remain unclear. In the present study, we found that C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper, suggested that the heat-labile components in serum are responsible for the immobilization of C. irritans. Moreover, we cloned and characterized the encoding sequence of pearl gentian grouper complement C3 (PGC3), a key component in complement system. We also found that the expression level of PGC3 was increased in infected grouper serum when compared with that of control grouper. Furthermore, the binding of PGC3 on the surface of C. irritans trophonts located on the grouper skin was detected. These data suggested that pearl gentian grouper's complement system indeed play roles in the immune response against the C. irritans infection. Pearl gentian grouper is a new aquacultural hybrid resulted from breeding of female tiger grouper (Epinephelus fuscogutattus) and male giant grouper (E. lanceolatus). Our preliminary study found that pearl gentian grouper exhibits less susceptible to the primary infection of Cryptocaryon irritans, which is an important parasitic ciliate in marine aquaculture, indicated that pearl gentian grouper might own a strong innate immune system. Complement system play key roles in innate immunity, whether pearl gentian grouper's complement component contribute for the defensing against the C. irritans infection remain unclear. In the present study, we found that C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper, suggested that the heat-labile components in serum are responsible for the immobilization of C. irritans. Moreover, we cloned and characterized the encoding sequence of pearl gentian grouper complement C3 (PGC3), a key component in complement system. We also found that the expression level of PGC3 was increased in infected grouper serum when compared with that of control grouper. Furthermore, the binding of PGC3 on the surface of C. irritans trophonts located on the grouper skin was detected. These data suggested that pearl gentian grouper's complement system indeed play roles in the immune response against the C. irritans infection.Pearl gentian grouper is a new aquacultural hybrid resulted from breeding of female tiger grouper (Epinephelus fuscogutattus) and male giant grouper (E. lanceolatus). Our preliminary study found that pearl gentian grouper exhibits less susceptible to the primary infection of Cryptocaryon irritans, which is an important parasitic ciliate in marine aquaculture, indicated that pearl gentian grouper might own a strong innate immune system. Complement system play key roles in innate immunity, whether pearl gentian grouper's complement component contribute for the defensing against the C. irritans infection remain unclear. In the present study, we found that C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper, suggested that the heat-labile components in serum are responsible for the immobilization of C. irritans. Moreover, we cloned and characterized the encoding sequence of pearl gentian grouper complement C3 (PGC3), a key component in complement system. We also found that the expression level of PGC3 was increased in infected grouper serum when compared with that of control grouper. Furthermore, the binding of PGC3 on the surface of C. irritans trophonts located on the grouper skin was detected. These data suggested that pearl gentian grouper's complement system indeed play roles in the immune response against the C. irritans infection. |
| ArticleNumber | 100032 |
| Author | Jiang, Biao Li, Yanwei Mo, Zequan Luo, Xiaochun Dan, Xueming Lai, Xueli Wu, Huicheng |
| Author_xml | – sequence: 1 givenname: Zequan surname: Mo fullname: Mo, Zequan organization: College of Marine Sciences, South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China – sequence: 2 givenname: Biao surname: Jiang fullname: Jiang, Biao organization: Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China – sequence: 3 givenname: Xueli surname: Lai fullname: Lai, Xueli organization: College of Marine Sciences, South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China – sequence: 4 givenname: Huicheng surname: Wu fullname: Wu, Huicheng organization: College of Marine Sciences, South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China – sequence: 5 givenname: Xiaochun surname: Luo fullname: Luo, Xiaochun organization: School of Bioscience and Biotechnology, South China University of Technology, Guangzhou 510006, China – sequence: 6 givenname: Xueming surname: Dan fullname: Dan, Xueming email: dxm72@scau.edu.cn organization: College of Marine Sciences, South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China – sequence: 7 givenname: Yanwei surname: Li fullname: Li, Yanwei email: yanweili@scau.edu.cn organization: College of Marine Sciences, South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China |
| BookMark | eNqFks9u1DAQxiNUJErpG3DwsRx2cf45MQcktCpQqRIXOFsTZ5z1ymsH26m0nBAvgbj1RbiUN-FJ8DYr1HKgp3jsb376MvM9zY6ss5hlz3O6zGnOXm6WKmiP47KgRZ6uKC2LR9lxwVizoHnOj-6cn2SnIWySpGh4RZv8OPu5WoMHGdHrLxC1swRsT9Rk5b4Ak0owu6ADcYqsd53XPRkRvCED2qjBksG7aURPzs5HbXFco5kCMWAlOgNxCr9_fLu5_vX95vruu5qCdMMU46z4-oJItx0NbhOUrEoCA2gb0tHvxugk-F1ypr3XEWwg2iq89fcse6zABDw9fE-yT2_PP67eLy4_vLtYvblcyLrkcQGtLGrIW1axrit6pmTNEdteUtXStlBdpVRZ9R0vOlB1R3MlUeZ12edJLYumPMkuZm7vYCNGr7fJkXCgxe2F84MAH7U0KFjdJXRXla1ileIcFC8rjqkAyrEqE-v1zBqnbou9TH_swdyD3n-xei0GdyU4ayltaAKcHQDefZ4wRLHVQaJJM0c3BZH88qainLcPS1nJWIpMu5dWs1R6F4JH9ddRTsU-aGIj5qCJfdDEHLTU9uqfNpl2tN9N8q7NQ82HUWDa3ZVGL4LUmJLTJ6mMabj6_4A_5NL_Lw |
| CitedBy_id | crossref_primary_10_1016_j_fbio_2024_105203 crossref_primary_10_1016_j_aquaculture_2024_741336 crossref_primary_10_1016_j_dci_2024_105253 |
| Cites_doi | 10.1016/j.fsi.2007.01.002 10.1016/j.fsi.2017.05.042 10.1016/j.fsi.2017.05.064 10.1016/j.fsi.2005.04.004 10.1016/j.dci.2017.06.009 10.1007/s00251-006-0096-3 10.1007/s00436-008-0904-9 10.1016/j.fsi.2021.09.041 10.1016/j.fsi.2016.01.022 10.1006/fsim.2001.0408 10.1038/cr.2009.139 10.1002/1521-4141(200003)30:3<858::AID-IMMU858>3.0.CO;2-M 10.1016/j.fsi.2016.09.059 10.1016/j.fsi.2011.09.018 10.1016/j.fsi.2019.02.039 10.1016/j.fsi.2018.03.039 10.1016/j.fsi.2012.12.011 10.1016/j.fsi.2008.05.010 10.1016/j.fsi.2006.04.011 10.1016/j.fsi.2018.02.044 10.1016/j.aquaculture.2006.04.026 10.1007/s00441-010-1034-0 10.1385/IR:27:2-3:549 10.1016/j.fsi.2011.02.004 10.1016/j.fsi.2012.07.005 10.1096/fj.08-110595 10.1016/j.molimm.2009.08.032 10.1016/j.fsi.2018.03.052 10.1016/j.fsi.2011.06.023 10.1038/s41598-018-25710-3 10.1007/s00251-005-0005-1 10.1023/A:1018360323287 10.1016/j.fsi.2014.06.018 |
| ContentType | Journal Article |
| Copyright | 2021 The Author(s) 2021 The Author(s). 2021 The Author(s) 2021 |
| Copyright_xml | – notice: 2021 The Author(s) – notice: 2021 The Author(s). – notice: 2021 The Author(s) 2021 |
| DBID | 6I. AAFTH AAYXX CITATION 7S9 L.6 7X8 5PM DOA |
| DOI | 10.1016/j.fsirep.2021.100032 |
| DatabaseName | ScienceDirect Open Access Titles Elsevier:ScienceDirect:Open Access CrossRef AGRICOLA AGRICOLA - Academic MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef AGRICOLA AGRICOLA - Academic MEDLINE - Academic |
| DatabaseTitleList | AGRICOLA MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Zoology |
| EISSN | 2667-0119 |
| ExternalDocumentID | oai_doaj_org_article_65bee8b438f64f99af9349ef64a09e43 PMC9680070 10_1016_j_fsirep_2021_100032 S266701192100027X |
| GroupedDBID | 6I. AAEDW AAFTH AAXUO AEXQZ ALMA_UNASSIGNED_HOLDINGS AMRAJ EBS FDB GROUPED_DOAJ M~E OK1 ROL RPM 0R~ AAHBH AALRI AAYWO AAYXX ACVFH ADCNI ADVLN AEUPX AFPUW AIGII AITUG AKBMS AKRWK AKYEP CITATION 7S9 L.6 7X8 5PM |
| ID | FETCH-LOGICAL-c539t-a8c25a18646bb2d6fc59ee8dc0f8082fb4ff34db92baf5b01fcec153d1b2dc273 |
| IEDL.DBID | DOA |
| ISSN | 2667-0119 |
| IngestDate | Wed Aug 27 01:29:33 EDT 2025 Thu Aug 21 18:39:51 EDT 2025 Fri Jul 11 09:15:47 EDT 2025 Thu Jul 10 23:32:24 EDT 2025 Thu Apr 24 23:10:38 EDT 2025 Tue Jul 01 03:09:01 EDT 2025 Fri Feb 23 02:41:05 EST 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | Pearl gentian grouper Complement C3 Cryptocaryon irritans |
| Language | English |
| License | This is an open access article under the CC BY-NC-ND license. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c539t-a8c25a18646bb2d6fc59ee8dc0f8082fb4ff34db92baf5b01fcec153d1b2dc273 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Zequan Mo and Biao Jiang have an equal dedication to this paper. |
| OpenAccessLink | https://doaj.org/article/65bee8b438f64f99af9349ef64a09e43 |
| PQID | 2636610088 |
| PQPubID | 24069 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_65bee8b438f64f99af9349ef64a09e43 pubmedcentral_primary_oai_pubmedcentral_nih_gov_9680070 proquest_miscellaneous_2739740998 proquest_miscellaneous_2636610088 crossref_primary_10_1016_j_fsirep_2021_100032 crossref_citationtrail_10_1016_j_fsirep_2021_100032 elsevier_sciencedirect_doi_10_1016_j_fsirep_2021_100032 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2021-12-01 |
| PublicationDateYYYYMMDD | 2021-12-01 |
| PublicationDate_xml | – month: 12 year: 2021 text: 2021-12-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationTitle | Fish and shellfish immunology reports |
| PublicationYear | 2021 |
| Publisher | Elsevier Ltd Elsevier |
| Publisher_xml | – name: Elsevier Ltd – name: Elsevier |
| References | Qu, Ricklin, Lambris (bib0005) 2009; 47 Jiang, Du, Li, Ma, Hu, Li (bib0033) 2019; 88 Hu, Li, Xu, Jiang, Lu, Luo (bib0030) 2017; 66 Mo, Xu, Cassidy-Hanley, Li, Kolbin, Fricke, Li, Clark, Dan (bib0025) 2019; 9 Luo, Xie, Zhu, Li (bib0028) 2007; 22 Lovoll, Kilvik, Boshra, Bogwald, Sunyer, Dalmo (bib0014) 2006; 58 Guardiola, Cuesta, Abellan, Meseguer, Esteban (bib0027) 2014; 40 Mishra, Sahoo, Mohanty, Das (bib0013) 2009; 47 Qi, Liu, Wang, Wu, Xin, Lu, Wang (bib0012) 2011; 31 Colorni, Burgess (bib0016) 1997; 1 Sunyer, Boshra, Lorenzo, Parra, Freedman, Bosch (bib0002) 2003; 27 Lovoll, Johnsen, Boshra, Bogwald, Sunyer, Dalmo (bib0015) 2007; 23 Nakao, Mutsuro, Obo, Fujiki, Nonaka, Yano (bib0010) 2000; 30 Sarma, Ward (bib0006) 2011; 343 Zhou, Sun, Yang, Dong, Chen, Wang, Guan, Jiang, Wang (bib0009) 2011; 31 Mo, Wu, Hu, Lu, Lai, Chen, He, Luo, Li, Dan (bib0021) 2021; 119 Luo, Xie, Zhu, Li (bib0018) 2008; 102 Mo, Han, Zeng, Wang, Li, Li, Sun, Li, Luo, Dan (bib0024) 2018; 77 Flierl, Rittirsch, Nadeau, Day, Zetoune, Sarma, Huber-Lang, Ward (bib0035) 2008; 22 Holland, Lambris (bib0001) 2002; 12 Mo, Wang, Yang, Ni, Wang, Lao, Li, Li, Luo, Dan (bib0022) 2017; 67 Dan, Zhang, Li, Li (bib0036) 2013; 34 Boshra, Li, Sunyer (bib0034) 2006; 20 Yin, Liu, Bao, Jin, Qian, Wang, Tang (bib0020) 2018; 77 Wang, Zhang, Wang, Zhang, Lv, Liu, Wang, Zhou, Qiu, Wang, Li, Song (bib0003) 2017; 76 Wang, Zhang, Wang (bib0011) 2008; 25 Khoo, Abdul-Murad, Kua, Mohd-Adnan (bib0029) 2012; 33 Yin, Gao, Tang, Sun, Han, Huang (bib0032) 2016; 50 Wang, Li, Xie, Li (bib0019) 2011; 30 Yin, Qian (bib0031) 2017 Dishaw, Smith, Bigger (bib0007) 2005; 57 Dan, Li, Lin, Teng, Zhu (bib0023) 2006; 258 Dunkelberger, Song (bib0004) 2010; 20 Cordero, Cuesta, Meseguer, Esteban (bib0026) 2016; 58 Li, Jiang, Mo, Li, Dan (bib0017) 2021 Chen, Xu, Li, Wang, Ye, Qi (bib0008) 2018; 76 Qi (10.1016/j.fsirep.2021.100032_bib0012) 2011; 31 Dan (10.1016/j.fsirep.2021.100032_bib0023) 2006; 258 Wang (10.1016/j.fsirep.2021.100032_bib0019) 2011; 30 Mo (10.1016/j.fsirep.2021.100032_bib0022) 2017; 67 Khoo (10.1016/j.fsirep.2021.100032_bib0029) 2012; 33 Mo (10.1016/j.fsirep.2021.100032_bib0024) 2018; 77 Hu (10.1016/j.fsirep.2021.100032_bib0030) 2017; 66 Luo (10.1016/j.fsirep.2021.100032_bib0028) 2007; 22 Nakao (10.1016/j.fsirep.2021.100032_bib0010) 2000; 30 Yin (10.1016/j.fsirep.2021.100032_bib0031) 2017 Qu (10.1016/j.fsirep.2021.100032_bib0005) 2009; 47 Jiang (10.1016/j.fsirep.2021.100032_bib0033) 2019; 88 Yin (10.1016/j.fsirep.2021.100032_bib0020) 2018; 77 Zhou (10.1016/j.fsirep.2021.100032_bib0009) 2011; 31 Colorni (10.1016/j.fsirep.2021.100032_bib0016) 1997; 1 Wang (10.1016/j.fsirep.2021.100032_bib0011) 2008; 25 Li (10.1016/j.fsirep.2021.100032_bib0017) 2021 Mo (10.1016/j.fsirep.2021.100032_bib0021) 2021; 119 Mishra (10.1016/j.fsirep.2021.100032_bib0013) 2009; 47 Lovoll (10.1016/j.fsirep.2021.100032_bib0014) 2006; 58 Lovoll (10.1016/j.fsirep.2021.100032_bib0015) 2007; 23 Cordero (10.1016/j.fsirep.2021.100032_bib0026) 2016; 58 Holland (10.1016/j.fsirep.2021.100032_bib0001) 2002; 12 Boshra (10.1016/j.fsirep.2021.100032_bib0034) 2006; 20 Dishaw (10.1016/j.fsirep.2021.100032_bib0007) 2005; 57 Yin (10.1016/j.fsirep.2021.100032_bib0032) 2016; 50 Guardiola (10.1016/j.fsirep.2021.100032_bib0027) 2014; 40 Sarma (10.1016/j.fsirep.2021.100032_bib0006) 2011; 343 Dan (10.1016/j.fsirep.2021.100032_bib0036) 2013; 34 Luo (10.1016/j.fsirep.2021.100032_bib0018) 2008; 102 Sunyer (10.1016/j.fsirep.2021.100032_bib0002) 2003; 27 Flierl (10.1016/j.fsirep.2021.100032_bib0035) 2008; 22 Dunkelberger (10.1016/j.fsirep.2021.100032_bib0004) 2010; 20 Wang (10.1016/j.fsirep.2021.100032_bib0003) 2017; 76 Mo (10.1016/j.fsirep.2021.100032_bib0025) 2019; 9 Chen (10.1016/j.fsirep.2021.100032_bib0008) 2018; 76 |
| References_xml | – volume: 27 start-page: 549 year: 2003 end-page: 564 ident: bib0002 article-title: Evolution of complement as an effector system in innate and adaptive immunity publication-title: Immunol. Res. – volume: 20 start-page: 34 year: 2010 end-page: 50 ident: bib0004 article-title: Complement and its role in innate and adaptive immune responses publication-title: Cell Res. – volume: 57 start-page: 535 year: 2005 end-page: 548 ident: bib0007 article-title: Characterization of a C3-like cDNA in a coral: phylogenetic implications publication-title: Immunogenetics – volume: 9 start-page: 1029 year: 2019 ident: bib0025 article-title: Characterization and immune regulation role of an immobilization antigen from publication-title: Sci. Rep. – volume: 77 start-page: 91 year: 2018 end-page: 99 ident: bib0024 article-title: Molecular characterization and function analysis of grouper ( publication-title: Fish Shellfish Immunol. – volume: 31 start-page: 1284 year: 2011 end-page: 1290 ident: bib0012 article-title: Molecular characterization and functional analysis of a complement C3 molecule in the orange-spotted grouper ( publication-title: Fish Shellfish Immunol. – volume: 47 start-page: 672 year: 2009 end-page: 678 ident: bib0013 article-title: Sequence information, ontogeny and tissue-specific expression of complement component C3 in Indian major carp, publication-title: Indian J. Exp. Biol. – year: 2021 ident: bib0017 article-title: (Brown, 1951) is a serious threat to aquaculture of marine fish publication-title: Rev. Aquacult. – volume: 30 start-page: 1095 year: 2011 end-page: 1108 ident: bib0019 article-title: The serum of rabbitfish ( publication-title: Fish Shellfish Immunol. – volume: 1 start-page: 217 year: 1997 end-page: 238 ident: bib0016 article-title: Brown 1951, the cause of ‘white spot disease’ in marine fish: an update publication-title: Aquarium Sci. Conserv. – volume: 40 start-page: 24 year: 2014 end-page: 31 ident: bib0027 article-title: Comparative analysis of the humoral immunity of skin mucus from several marine teleost fish publication-title: Fish Shellfish Immunol. – volume: 25 start-page: 264 year: 2008 end-page: 270 ident: bib0011 article-title: Response of complement expression to challenge with lipopolysaccharide in embryos/larvae of zebrafish publication-title: Fish Shellfish Immunol. – volume: 47 start-page: 185 year: 2009 end-page: 195 ident: bib0005 article-title: Recent developments in low molecular weight complement inhibitors publication-title: Mol. Immunol. – volume: 58 start-page: 500 year: 2016 end-page: 507 ident: bib0026 article-title: Changes in the levels of humoral immune activities after storage of gilthead seabream ( publication-title: Fish Shellfish Immunol. – volume: 119 start-page: 67 year: 2021 end-page: 75 ident: bib0021 article-title: Transcriptomic analysis reveals innate immune mechanisms of an underlying parasite-resistant grouper hybrid (Epinephelus fuscogutatus × Epinephelus lanceolatus) publication-title: Fish Shellfish Immunol. – volume: 12 start-page: 399 year: 2002 end-page: 420 ident: bib0001 article-title: The complement system in teleosts publication-title: Fish Shellfish Immunol. – volume: 102 start-page: 1269 year: 2008 end-page: 1275 ident: bib0018 article-title: Some characteristics of host-parasite relationship for publication-title: Parasitol. Res. – volume: 258 start-page: 127 year: 2006 end-page: 133 ident: bib0023 article-title: A standardized method to propagate publication-title: Aquaculture – volume: 22 start-page: 427 year: 2007 end-page: 432 ident: bib0028 article-title: Protective immunity in grouper ( publication-title: Fish Shellfish Immunol. – volume: 66 start-page: 398 year: 2017 end-page: 410 ident: bib0030 article-title: Transcriptomic variation of locally-infected skin of publication-title: Fish Shellfish Immunol. – volume: 23 start-page: 542 year: 2007 end-page: 552 ident: bib0015 article-title: The ontogeny and extrahepatic expression of complement factor C3 in Atlantic salmon ( publication-title: Fish Shellfish Immunol. – volume: 76 start-page: 209 year: 2017 end-page: 219 ident: bib0003 article-title: The RNA-seq analysis suggests a potential multi-component complement system in oyster publication-title: Dev. Comp. Immunol. – volume: 34 start-page: 885 year: 2013 end-page: 891 ident: bib0036 article-title: Immune responses and immune-related gene expression profile in orange-spotted grouper after immunization with publication-title: Fish Shellfish Immunol. – volume: 77 start-page: 298 year: 2018 end-page: 303 ident: bib0020 article-title: Comparison of the susceptibility and resistance of four marine perciform fishes to publication-title: Fish Shellfish Immunol. – start-page: 10 year: 2017 ident: bib0031 article-title: Transcriptomic analysis reveals the key immune-related signalling pathways of publication-title: Parasit. Vectors – volume: 33 start-page: 788 year: 2012 end-page: 794 ident: bib0029 article-title: infection induces the acute phase response in publication-title: Fish Shellfish Immunol. – volume: 30 start-page: 858 year: 2000 end-page: 866 ident: bib0010 article-title: Molecular cloning and protein analysis of divergent forms of the complement component C3 from a bony fish, the common carp ( publication-title: Eur. J. Immunol. – volume: 22 start-page: 3483 year: 2008 end-page: 3490 ident: bib0035 article-title: Functions of the complement components C3 and C5 during sepsis publication-title: FASEB J. – volume: 76 start-page: 41 year: 2018 end-page: 47 ident: bib0008 article-title: Molecular characterization of complement component 3 (C3) in publication-title: Fish Shellfish Immunol. – volume: 31 start-page: 540 year: 2011 end-page: 547 ident: bib0009 article-title: Molecular characterization and expression analysis of a complement component 3 in the sea cucumber ( publication-title: Fish Shellfish Immunol. – volume: 88 start-page: 111 year: 2019 end-page: 116 ident: bib0033 article-title: Transcriptome analysis provides insights into molecular immune mechanisms of rabbitfish, publication-title: Fish Shellfish Immunol. – volume: 343 start-page: 227 year: 2011 end-page: 235 ident: bib0006 article-title: The complement system publication-title: Cell Tissue Res. – volume: 67 start-page: 467 year: 2017 end-page: 474 ident: bib0022 article-title: Characterization and expression analysis of grouper ( publication-title: Fish Shellfish Immunol. – volume: 50 start-page: 127 year: 2016 end-page: 141 ident: bib0032 article-title: Transcriptome and analysis on the complement and coagulation cascades pathway of large yellow croaker ( publication-title: Fish Shellfish Immunol. – volume: 20 start-page: 239 year: 2006 end-page: 262 ident: bib0034 article-title: Recent advances on the complement system of teleost fish publication-title: Fish Shellfish Immunol. – volume: 58 start-page: 168 year: 2006 end-page: 179 ident: bib0014 article-title: Maternal transfer of complement components C3-1, C3-3, C3-4, C4, C5, C7, Bf, and Df to offspring in rainbow trout ( publication-title: Immunogenetics – volume: 23 start-page: 542 issue: 3 year: 2007 ident: 10.1016/j.fsirep.2021.100032_bib0015 article-title: The ontogeny and extrahepatic expression of complement factor C3 in Atlantic salmon (Salmo salar) publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2007.01.002 – volume: 66 start-page: 398 year: 2017 ident: 10.1016/j.fsirep.2021.100032_bib0030 article-title: Transcriptomic variation of locally-infected skin of Epinephelus coioides reveals the mucosal immune mechanism against Cryptocaryon irritans publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2017.05.042 – volume: 67 start-page: 467 year: 2017 ident: 10.1016/j.fsirep.2021.100032_bib0022 article-title: Characterization and expression analysis of grouper (Epinephelus coioides) co-stimulatory molecules CD83 and CD80/86 post Cryptocaryon irritans infection publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2017.05.064 – volume: 20 start-page: 239 issue: 2 year: 2006 ident: 10.1016/j.fsirep.2021.100032_bib0034 article-title: Recent advances on the complement system of teleost fish publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2005.04.004 – volume: 76 start-page: 209 year: 2017 ident: 10.1016/j.fsirep.2021.100032_bib0003 article-title: The RNA-seq analysis suggests a potential multi-component complement system in oyster Crassostrea gigas publication-title: Dev. Comp. Immunol. doi: 10.1016/j.dci.2017.06.009 – volume: 58 start-page: 168 issue: 2–3 year: 2006 ident: 10.1016/j.fsirep.2021.100032_bib0014 article-title: Maternal transfer of complement components C3-1, C3-3, C3-4, C4, C5, C7, Bf, and Df to offspring in rainbow trout (Oncorhynchus mykiss) publication-title: Immunogenetics doi: 10.1007/s00251-006-0096-3 – volume: 102 start-page: 1269 issue: 6 year: 2008 ident: 10.1016/j.fsirep.2021.100032_bib0018 article-title: Some characteristics of host-parasite relationship for Cryptocaryon irritans isolated from South China publication-title: Parasitol. Res. doi: 10.1007/s00436-008-0904-9 – volume: 119 start-page: 67 year: 2021 ident: 10.1016/j.fsirep.2021.100032_bib0021 article-title: Transcriptomic analysis reveals innate immune mechanisms of an underlying parasite-resistant grouper hybrid (Epinephelus fuscogutatus × Epinephelus lanceolatus) publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2021.09.041 – volume: 50 start-page: 127 year: 2016 ident: 10.1016/j.fsirep.2021.100032_bib0032 article-title: Transcriptome and analysis on the complement and coagulation cascades pathway of large yellow croaker (Larimichthys crocea) to ciliate ectoparasite Cryptocaryon irritans infection publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2016.01.022 – volume: 12 start-page: 399 issue: 5 year: 2002 ident: 10.1016/j.fsirep.2021.100032_bib0001 article-title: The complement system in teleosts publication-title: Fish Shellfish Immunol. doi: 10.1006/fsim.2001.0408 – volume: 20 start-page: 34 issue: 1 year: 2010 ident: 10.1016/j.fsirep.2021.100032_bib0004 article-title: Complement and its role in innate and adaptive immune responses publication-title: Cell Res. doi: 10.1038/cr.2009.139 – start-page: 10 year: 2017 ident: 10.1016/j.fsirep.2021.100032_bib0031 article-title: Transcriptomic analysis reveals the key immune-related signalling pathways of Sebastiscus marmoratus in response to infection with the parasitic ciliate Cryptocaryon irritans publication-title: Parasit. Vectors – volume: 30 start-page: 858 issue: 3 year: 2000 ident: 10.1016/j.fsirep.2021.100032_bib0010 article-title: Molecular cloning and protein analysis of divergent forms of the complement component C3 from a bony fish, the common carp (Cyprinus carpio): presence of variants lacking the catalytic histidine publication-title: Eur. J. Immunol. doi: 10.1002/1521-4141(200003)30:3<858::AID-IMMU858>3.0.CO;2-M – volume: 58 start-page: 500 year: 2016 ident: 10.1016/j.fsirep.2021.100032_bib0026 article-title: Changes in the levels of humoral immune activities after storage of gilthead seabream (Sparus aurata) skin mucus publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2016.09.059 – volume: 31 start-page: 1284 issue: 6 year: 2011 ident: 10.1016/j.fsirep.2021.100032_bib0012 article-title: Molecular characterization and functional analysis of a complement C3 molecule in the orange-spotted grouper (Epinephelus coioides) publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2011.09.018 – volume: 88 start-page: 111 year: 2019 ident: 10.1016/j.fsirep.2021.100032_bib0033 article-title: Transcriptome analysis provides insights into molecular immune mechanisms of rabbitfish, Siganus oramin against Cryptocaryon irritans infection publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2019.02.039 – volume: 77 start-page: 91 year: 2018 ident: 10.1016/j.fsirep.2021.100032_bib0024 article-title: Molecular characterization and function analysis of grouper (Epinephelus coioides) Bruton’s tyrosine kinase BTK publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2018.03.039 – volume: 34 start-page: 885 issue: 3 year: 2013 ident: 10.1016/j.fsirep.2021.100032_bib0036 article-title: Immune responses and immune-related gene expression profile in orange-spotted grouper after immunization with Cryptocaryon irritans vaccine publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2012.12.011 – volume: 25 start-page: 264 issue: 3 year: 2008 ident: 10.1016/j.fsirep.2021.100032_bib0011 article-title: Response of complement expression to challenge with lipopolysaccharide in embryos/larvae of zebrafish Danio rerio: acquisition of immunocompetent complement publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2008.05.010 – volume: 22 start-page: 427 issue: 4 year: 2007 ident: 10.1016/j.fsirep.2021.100032_bib0028 article-title: Protective immunity in grouper (Epinephelus coioides) following exposure to or injection with Cryptocaryon irritans publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2006.04.011 – volume: 76 start-page: 41 year: 2018 ident: 10.1016/j.fsirep.2021.100032_bib0008 article-title: Molecular characterization of complement component 3 (C3) in Mytilus coruscus improves our understanding of bivalve complement system publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2018.02.044 – year: 2021 ident: 10.1016/j.fsirep.2021.100032_bib0017 article-title: Cryptocaryon irritans (Brown, 1951) is a serious threat to aquaculture of marine fish publication-title: Rev. Aquacult. – volume: 258 start-page: 127 issue: 1–4 year: 2006 ident: 10.1016/j.fsirep.2021.100032_bib0023 article-title: A standardized method to propagate Cryptocaryon irritans on a susceptible host pompano Trachinotus ovatus publication-title: Aquaculture doi: 10.1016/j.aquaculture.2006.04.026 – volume: 343 start-page: 227 issue: 1 year: 2011 ident: 10.1016/j.fsirep.2021.100032_bib0006 article-title: The complement system publication-title: Cell Tissue Res. doi: 10.1007/s00441-010-1034-0 – volume: 27 start-page: 549 issue: 2–3 year: 2003 ident: 10.1016/j.fsirep.2021.100032_bib0002 article-title: Evolution of complement as an effector system in innate and adaptive immunity publication-title: Immunol. Res. doi: 10.1385/IR:27:2-3:549 – volume: 30 start-page: 1095 issue: 4–5 year: 2011 ident: 10.1016/j.fsirep.2021.100032_bib0019 article-title: The serum of rabbitfish (Siganus oramin) has antimicrobial activity to some pathogenic organisms and a novel serum l-amino acid oxidase is isolated publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2011.02.004 – volume: 33 start-page: 788 issue: 4 year: 2012 ident: 10.1016/j.fsirep.2021.100032_bib0029 article-title: Cryptocaryon irritans infection induces the acute phase response in Lates calcarifer: a transcriptomic perspective publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2012.07.005 – volume: 22 start-page: 3483 issue: 10 year: 2008 ident: 10.1016/j.fsirep.2021.100032_bib0035 article-title: Functions of the complement components C3 and C5 during sepsis publication-title: FASEB J. doi: 10.1096/fj.08-110595 – volume: 47 start-page: 185 issue: 2–3 year: 2009 ident: 10.1016/j.fsirep.2021.100032_bib0005 article-title: Recent developments in low molecular weight complement inhibitors publication-title: Mol. Immunol. doi: 10.1016/j.molimm.2009.08.032 – volume: 77 start-page: 298 year: 2018 ident: 10.1016/j.fsirep.2021.100032_bib0020 article-title: Comparison of the susceptibility and resistance of four marine perciform fishes to Cryptocaryon irritans infection publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2018.03.052 – volume: 31 start-page: 540 issue: 4 year: 2011 ident: 10.1016/j.fsirep.2021.100032_bib0009 article-title: Molecular characterization and expression analysis of a complement component 3 in the sea cucumber (Apostichopus japonicus) publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2011.06.023 – volume: 9 start-page: 1029 issue: 1 year: 2019 ident: 10.1016/j.fsirep.2021.100032_bib0025 article-title: Characterization and immune regulation role of an immobilization antigen from Cryptocaryon irritans on groupers publication-title: Sci. Rep. doi: 10.1038/s41598-018-25710-3 – volume: 57 start-page: 535 issue: 7 year: 2005 ident: 10.1016/j.fsirep.2021.100032_bib0007 article-title: Characterization of a C3-like cDNA in a coral: phylogenetic implications publication-title: Immunogenetics doi: 10.1007/s00251-005-0005-1 – volume: 1 start-page: 217 issue: 4 year: 1997 ident: 10.1016/j.fsirep.2021.100032_bib0016 article-title: Cryptocaryon irritans Brown 1951, the cause of ‘white spot disease’ in marine fish: an update publication-title: Aquarium Sci. Conserv. doi: 10.1023/A:1018360323287 – volume: 40 start-page: 24 issue: 1 year: 2014 ident: 10.1016/j.fsirep.2021.100032_bib0027 article-title: Comparative analysis of the humoral immunity of skin mucus from several marine teleost fish publication-title: Fish Shellfish Immunol. doi: 10.1016/j.fsi.2014.06.018 – volume: 47 start-page: 672 issue: 8 year: 2009 ident: 10.1016/j.fsirep.2021.100032_bib0013 article-title: Sequence information, ontogeny and tissue-specific expression of complement component C3 in Indian major carp, Labeo rohita (Hamilton) publication-title: Indian J. Exp. Biol. |
| SSID | ssj0002794071 |
| Score | 2.2148774 |
| Snippet | •C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper.•The expression level of PGC3 was increased in... Pearl gentian grouper is a new aquacultural hybrid resulted from breeding of female tiger grouper (Epinephelus fuscogutattus) and male giant grouper (E.... • C. irritans can be immobilized by untreated serum but not heat-treated serum from pearl gentian grouper. • The expression level of PGC3 was increased in... |
| SourceID | doaj pubmedcentral proquest crossref elsevier |
| SourceType | Open Website Open Access Repository Aggregation Database Enrichment Source Index Database Publisher |
| StartPage | 100032 |
| SubjectTerms | blood serum complement Complement C3 Cryptocaryon irritans females grouper heat treatment hybrids immune response innate immunity males mariculture Mycteroperca tigris Pearl gentian grouper shellfish |
| Title | Characterization and functional analysis of hybrid pearl gentian grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) complement C3 against Cryptocaryon irritans infection |
| URI | https://dx.doi.org/10.1016/j.fsirep.2021.100032 https://www.proquest.com/docview/2636610088 https://www.proquest.com/docview/2739740998 https://pubmed.ncbi.nlm.nih.gov/PMC9680070 https://doaj.org/article/65bee8b438f64f99af9349ef64a09e43 |
| Volume | 2 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV27itwwFBVhIZAmZPMgkxcKpEgKEz9k2SqTYZYlsKmysKQxki3tzDLYxo9imhD2J0K6_ZE0mz_Jl-ReyR7sJtOkMci-M37oWPfo-t4jQt4wiU5cBV4IXNRjKdeeLDTztIa2SriONAb0zz7z03P26SK-mCz1hTlhTh7YPbj3PFZap4pFqeHMCCGNiJjQ0JC-0MzqfILPm0ymruznNIEzFVxZjnPMrQzEWDdnk7tMCwMKylWGAeYJ-FE480tWvn_mnib0c548OfFGJw_I_YFG0g_u8o_JHV0-JHe_VjZI_oj8Wu51mF2ZJZVlQdGHudAfNJ0WCa0MXe-waovWgPktxUorQAy11R66oW9XNfDQeq23fUu3CBGYC3d9--fn9e3N7x-3N9Pjpm_z6rLvOmfx_R21Ges2AkmXEZWXcgN0lC6bXd2BE212cGWbBuMTZUvHvLDyMTk_WX1ZnnrDQg1eHkei82Sah7EMUs64UmHBTR4L6LQi900KFMMoZkzECiVCJU2s_MDkOoehtgjAOgcC9YQclVWpnxIaplIgp1AGuCRwVWVgNA5zXwo4RWySBYnGbsryQcUcF9PYZmO62lXmOjfDzs1c5y6It_9V7VQ8Dth_RATsbVGD2-4AZGYDMrNDyFyQZMRPNtAZR1PgrzYHTv96hFsGbzt-wpGlrvo2C3kEhAp4W_oPmwQ4JkzbBdgkM6zO7md-pNysrba44CkqQD37Hw_gObmHN-WSf16Qo67p9UugcJ16Zd9W2J59W_0FBdlQhg |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Characterization+and+functional+analysis+of+hybrid+pearl+gentian+grouper+%28Epinephelus+lanceolatus%E2%99%82%C2%A0%C3%97%C2%A0Epinephelus+fuscoguttatus%E2%99%80%29+complement+C3+against+Cryptocaryon+irritans+infection&rft.jtitle=Fish+and+shellfish+immunology+reports&rft.au=Mo%2C+Zequan&rft.au=Jiang%2C+Biao&rft.au=Lai%2C+Xueli&rft.au=Wu%2C+Huicheng&rft.date=2021-12-01&rft.pub=Elsevier&rft.eissn=2667-0119&rft.volume=2&rft_id=info:doi/10.1016%2Fj.fsirep.2021.100032&rft.externalDocID=PMC9680070 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2667-0119&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2667-0119&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2667-0119&client=summon |