Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cD...

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Published inAnimal (Cambridge, England) Vol. 7; no. 5; pp. 778 - 783
Main Authors Collares, T. F., Campos, V. F., Urtiaga, G., Leon, P. M. M., Amaral, M. G., Hartleben, C. P., McBride, A. J., Dellagostin, O. A., Deschamps, J. C., Seixas, F. K., Collares, T.
Format Journal Article
LanguageEnglish
Published Cambridge, UK Cambridge University Press 01.05.2013
Elsevier Limited
Elsevier
Subjects
Animals
Cloning, Molecular
complementary DNA
EPO
Erythrocytes
erythropoietin
Erythropoietin - genetics
gene therapy
gene transfer
Genetic Therapy - methods
Genetic Therapy - veterinary
HeLa Cells
hematocrit
Humans
Male
Physiology and functional biology of systems
plasmid DNA
Rabbits
reproductive performance
rHuEPO
sperm characteristics
sperm motility
Sperm Motility - physiology
spermatozoa
Spermatozoa - cytology
Spermatozoa - physiology
testes
Testis
viability
vigor
rHuEPO
plasmid DNA
EPO
sperm characteristics
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Abstract Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P < 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P < 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P > 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis.
AbstractList Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P < 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P < 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P > 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis.
Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P < 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P < 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P > 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis. [PUBLICATION ABSTRACT]
Author Hartleben, C. P.
McBride, A. J.
Collares, T.
Dellagostin, O. A.
Leon, P. M. M.
Urtiaga, G.
Collares, T. F.
Campos, V. F.
Amaral, M. G.
Seixas, F. K.
Deschamps, J. C.
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/23171533$$D View this record in MEDLINE/PubMed
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DocumentTitleAlternate Collares, Campos, Urtiaga, Leon, Amaral, Hartleben, McBride, Dellagostin, Deschamps, Seixas and Collares
Sperm characteristics after rabbit EPO gene therapy
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plasmid DNA
EPO
sperm characteristics
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Snippet Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to...
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StartPage 778
SubjectTerms Animals
Cloning, Molecular
complementary DNA
EPO
Erythrocytes
erythropoietin
Erythropoietin - genetics
gene therapy
gene transfer
Genetic Therapy - methods
Genetic Therapy - veterinary
HeLa Cells
hematocrit
Humans
Male
Physiology and functional biology of systems
plasmid DNA
Rabbits
reproductive performance
rHuEPO
sperm characteristics
sperm motility
Sperm Motility - physiology
spermatozoa
Spermatozoa - cytology
Spermatozoa - physiology
testes
Testis
viability
vigor
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Title Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm
URI https://www.cambridge.org/core/product/identifier/S1751731112002157/type/journal_article
https://www.ncbi.nlm.nih.gov/pubmed/23171533
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