Rifampin phosphotransferase is an unusual antibiotic resistance kinase

• Published in: Nature communications Vol. 7; no. 1; pp. 11343 - 12
• Main Authors: Stogios, Peter J., Cox, Georgina, Spanogiannopoulos, Peter, Pillon, Monica C., Waglechner, Nicholas, Skarina, Tatiana, Koteva, Kalinka, Guarné, Alba, Savchenko, Alexei, Wright, Gerard D.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.04.2016; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326/41/2536; 631/45/607/275; 82/16; 82/58; 82/80; Adenosine Triphosphate - chemistry; Adenosine Triphosphate - metabolism; Amino Acid Sequence; Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; ATP; Bacteria; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Binding Sites; Biochemistry; Biotransformation; Catalysis; Crystallography, X-Ray; Drug resistance; Drug Resistance, Bacterial; Enzymes; Escherichia coli - genetics; Escherichia coli - metabolism; Gene Expression; Genomes; Humanities and Social Sciences; Kinases; Listeria; Listeria monocytogenes - classification; Listeria monocytogenes - drug effects; Listeria monocytogenes - enzymology; Listeria monocytogenes - genetics; Models, Molecular; Molecular Sequence Data; multidisciplinary; Phosphorylation; Phosphotransferases - chemistry; Phosphotransferases - genetics; Phosphotransferases - metabolism; Phylogeny; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Rifampin - metabolism; Rifampin - pharmacology; Science; Science (multidisciplinary); Sequence Alignment; Ontario Canada; Canada

Rifampin (RIF) phosphotransferase (RPH) confers antibiotic resistance by conversion of RIF and ATP, to inactive phospho-RIF, AMP and P i . Here we present the crystal structure of RPH from Listeria monocytogenes (RPH- Lm ), which reveals that the enzyme is comprised of three domains: two substrate-b...