Single-cell force spectroscopy as a technique to quantify human red blood cell adhesion to subendothelial laminin

• Published in: Journal of biomechanics Vol. 47; no. 16; pp. 3855 - 3861
• Main Authors: Maciaszek, Jamie L., Partola, Kostyantyn, Zhang, Jing, Andemariam, Biree, Lykotrafitis, George
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 18.12.2014; Elsevier Limited
• Subjects: Adhesion; Adhesive strength; Atomic force microscopy; BCAM/Lu; Blood; Cell Adhesion; Cell Adhesion Molecules - physiology; Cyclic AMP - metabolism; Cytoadhesion; Erythrocyte; Erythrocytes - physiology; Experiments; Glass substrates; Healthy Volunteers; Human; Human subjects; Humans; Kinases; Laminin; Laminin - physiology; Ligands; Lutheran Blood-Group System - physiology; Membrane Glycoproteins; Microscopy, Atomic Force - methods; Physical Medicine and Rehabilitation; Protein Binding; Proteins; Red blood cells; Silicon nitride; Single molecule force spectroscopy; Spectrum analysis; Spectrum Analysis - methods; Studies; Erythrocyte; Single molecule force spectroscopy; Atomic force microscopy; BCAM/Lu; Cytoadhesion

Single-cell force spectroscopy (SCFS), an atomic force microscopy (AFM)-based assay, enables quantitative study of cell adhesion while maintaining the native state of surface receptors in physiological conditions. Human healthy and pathological red blood cells (RBCs) express a large number of surfac...