A quantitative framework reveals ecological drivers of grassland microbial community assembly in response to warming

• Published in: Nature communications Vol. 11; no. 1; pp. 4717 - 12
• Main Authors: Ning, Daliang, Yuan, Mengting, Wu, Linwei, Zhang, Ya, Guo, Xue, Zhou, Xishu, Yang, Yunfeng, Arkin, Adam P., Firestone, Mary K., Zhou, Jizhong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.09.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 49/22; 49/23; 631/158/2453; 631/326/2565; 704/158/853; 704/158/855; Animals; Bacteria - classification; BASIC BIOLOGICAL SCIENCES; Biodiversity; community ecology; Droughts; Ecology; Global Warming; Grassland; grassland ecology; Humanities and Social Sciences; Microbial communities; microbial ecology; Microbiota; Models, Biological; multidisciplinary; Phylogeny; Science; Science (multidisciplinary); Sensitivity and Specificity
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-18560-z

Unraveling the drivers controlling community assembly is a central issue in ecology. Although it is generally accepted that selection, dispersal, diversification and drift are major community assembly processes, defining their relative importance is very challenging. Here, we present a framework to quantitatively infer community assembly mechanisms by phylogenetic bin-based null model analysis (iCAMP). iCAMP shows high accuracy (0.93–0.99), precision (0.80–0.94), sensitivity (0.82–0.94), and specificity (0.95–0.98) on simulated communities, which are 10–160% higher than those from the entire community-based approach. Application of iCAMP to grassland microbial communities in response to experimental warming reveals dominant roles of homogeneous selection (38%) and ‘drift’ (59%). Interestingly, warming decreases ‘drift’ over time, and enhances homogeneous selection which is primarily imposed on Bacillales. In addition, homogeneous selection has higher correlations with drought and plant productivity under warming than control. iCAMP provides an effective and robust tool to quantify microbial assembly processes, and should also be useful for plant and animal ecology. Studies of microbial community assembly mechanisms typically use metrics for turnover within the whole community. Here, the authors develop an alternative approach based on turnover within lineages and dissect mechanistic change in grassland bacterial assembly under experimental warming.