Association of Mycobacterium tuberculosis PE_PGRS33 polymorphism with clinical and epidemiological characteristics
Summary There is evidence that some members of the Mycobacterium tuberculosis PE_PGRS gene subfamily, including PE_PGRS33, may have a specific function in M. tuberculosis persistence. The impact of naturally-occurring PE_PGRS33 genetic variations on the virulence and transmissibility of clinical M....
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Published in | Tuberculosis (Edinburgh, Scotland) Vol. 87; no. 4; pp. 338 - 346 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Scotland
Elsevier Ltd
01.07.2007
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Subjects | |
Online Access | Get full text |
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Summary: | Summary There is evidence that some members of the Mycobacterium tuberculosis PE_PGRS gene subfamily, including PE_PGRS33, may have a specific function in M. tuberculosis persistence. The impact of naturally-occurring PE_PGRS33 genetic variations on the virulence and transmissibility of clinical M. tuberculosis isolates is not known. We used PCR and DNA sequencing to identify genetic variations in the PE_PGRS33 gene in comparison with the sequenced laboratory strain, H37Rv, among 649 isolates from a population-based sample. The PE_PGRS33 alleles were placed into two groups, based on the effect of the sequence variations on the PE_PGRS33 protein, and their associations with clinical and epidemiological characteristics were assessed using multivariate logistic regression to control for potential confounding of host-related factors. Of the 639 isolates for which sequence data were obtained, 139 (21.8%) had PE_PGRS33 alleles that would result in a significant change to the PE_PGRS33 protein due to large insertions/deletions or frameshift mutations. These isolates were significantly associated with clustering based on genotype and absence of cavitations in the lungs, compared to isolates having PE_PGRS33 alleles that would result in no or minimal change to the PE_PGRS33 protein. The association of significant changes to PE_PGRS33 with clinical and epidemiological characteristics suggests that PE_PGRS33 may have an important role in M. tuberculosis persistence. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1472-9792 1873-281X |
DOI: | 10.1016/j.tube.2007.03.003 |