CRISPR interference-based specific and efficient gene inactivation in the brain

• Published in: Nature neuroscience Vol. 21; no. 3; pp. 447 - 454
• Main Authors: Zheng, Yi, Shen, Wei, Zhang, Jian, Yang, Bo, Liu, Yao-Nan, Qi, Huihui, Yu, Xia, Lu, Si-Yao, Chen, Yun, Xu, Yu-Zhou, Li, Yun, Gage, Fred H., Mi, Shuangli, Yao, Jun
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2018; Nature Publishing Group
• Subjects: 13/51; 14/19; 42/41; 45/15; 631/337; 631/378/340; 64/60; 9/74; Analysis; Animal genetic engineering; Animal Genetics and Genomics; Artificial neural networks; Behavioral Sciences; Biological Techniques; Biomedical and Life Sciences; Biomedicine; Brain; Cell lines; Comparative analysis; CRISPR; CRISPR-Cas systems; Deactivation; Dentate gyrus; Fishing; Fishing (Recreation); Gene silencing; Genes; Genetic aspects; Genetic engineering; Genetic modification; Genome editing; Inactivation; Interference; Neural networks; Neurobiology; Neurons; Neurosciences; Neurotransmitter release; Synaptotagmin; technical-report

CRISPR–Cas9 has been demonstrated to delete genes in postmitotic neurons. Compared to the establishment of proliferative cell lines or animal strains, it is more challenging to acquire a highly homogeneous consequence of gene editing in a stable neural network. Here we show that dCas9-based CRISPR i...