Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells

• Published in: Genes to cells : devoted to molecular & cellular mechanisms Vol. 14; no. 12; pp. 1395 - 1404
• Main Authors: Nagata, Shogo, Toyoda, Masashi, Yamaguchi, Shinpei, Hirano, Kunio, Makino, Hatsune, Nishino, Koichiro, Miyagawa, Yoshitaka, Okita, Hajime, Kiyokawa, Nobutaka, Nakagawa, Masato, Yamanaka, Shinya, Akutsu, Hidenori, Umezawa, Akihiro, Tada, Takashi
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.12.2009; Blackwell Publishing Ltd
• Subjects: Amnion - cytology; Amnion - metabolism; Animals; Biomarkers - metabolism; Cell Differentiation; Cellular Reprogramming - physiology; Chimera; DNA Methylation; Embryonic Stem Cells - physiology; Female; Gene Expression Profiling; Gene Expression Regulation, Developmental; Humans; Immunoenzyme Techniques; Infant, Newborn; Male; Mice; Mice, Transgenic; Pluripotent Stem Cells - physiology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Teratoma - metabolism; Teratoma - pathology; Yolk Sac - cytology; Yolk Sac - metabolism

Practical clinical applications for current induced pluripotent stem cell (iPSC) technologies are hindered by very low generation efficiencies. Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc an...