Solitary HERV-K LTRs possess bi-directional promoter activity and contain a negative regulatory element in the U5 region

Reporter gene analysis of HERV-K solitary long terminal repeats (LTRs) showed that they retain detectable activity in human teratocarcinoma cells, and can direct the transcription in both orientations relative to the reporter gene. Deletion analysis demonstrated the possible existence of alternative...

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Bibliographic Details
Published inFEBS letters Vol. 472; no. 2-3; pp. 191 - 195
Main Authors Domansky, Andrey N., Kopantzev, Eugene P., Snezhkov, Eugene V., Lebedev, Yuri B., Leib-Mosch, Christine, Sverdlov, Eugene D.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 28.04.2000
Subjects
Endogenous Retroviruses - genetics
HERV-K
Human endogenous retrovirus
Humans
Long terminal repeat
Promoter Regions, Genetic
Regulation of transcription
Regulatory Sequences, Nucleic Acid
Terminal Repeat Sequences
Tumor Cells, Cultured
HERV-K
Human endogenous retrovirus
Regulation of transcription
Long terminal repeat
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Summary:Reporter gene analysis of HERV-K solitary long terminal repeats (LTRs) showed that they retain detectable activity in human teratocarcinoma cells, and can direct the transcription in both orientations relative to the reporter gene. Deletion analysis demonstrated the possible existence of alternative promoters within the LTR as well as a silencer-like element in the U5 region. Our results indicate also that all-trans-retinoic acid is capable of modulating expression of the reporter gene directed by a HERV-K LTR in NT2/D1 cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(00)01460-5