Gene expression profiling: monitoring transcription and translation products using DNA microarrays and proteomics

• Published in: FEBS Letters Vol. 480; no. 1; pp. 2 - 16
• Main Authors: Celis, Julio E, Kruhøffer, Mogens, Gromova, Irina, Frederiksen, Casper, Østergaard, Morten, Thykjaer, Thomas, Gromov, Pavel, Yu, Jinsheng, Pálsdóttir, Hildur, Magnusson, Nils, Ørntoft, Torben F
• Format: Book Review Journal Article
• Language: English
• Published: England Elsevier B.V 25.08.2000
• Subjects: 2D PAGE, two-dimensional polyacrylamide gel electrophoresis; Animals; Biopsy; Bladder cancer; Computational Biology - methods; Databases as Topic; Disease Models, Animal; Electrophoresis, Gel, Two-Dimensional - methods; Gene expression profile; Gene Expression Profiling - methods; Humans; IEF, isoelectric focusing; Immunohistochemistry; IPG, immobilized pH gradient; Microarray; Neoplasms - genetics; Neoplasms - metabolism; Neoplasms - pathology; NEPHGE, non-equilibrium pH gradient electrophoresis; Oligonucleotide Array Sequence Analysis - methods; Proteins - analysis; Proteins - genetics; Proteome; Transcription, Genetic - genetics; Two-dimensional gel electrophoresis; Immunohistochemistry; NEPHGE, non-equilibrium pH gradient electrophoresis; IPG, immobilized pH gradient; 2D PAGE, two-dimensional polyacrylamide gel electrophoresis; Two-dimensional gel electrophoresis; Proteome; Microarray; IEF, isoelectric focusing; Bladder cancer; Gene expression profile
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(00)01771-3

Novel and powerful technologies such as DNA microarrays and proteomics have made possible the analysis of the expression levels of multiple genes simultaneously both in health and disease. In combination, these technologies promise to revolutionize biology, in particular in the area of molecular medicine as they are expected to reveal gene regulation events involved in disease progression as well as to pinpoint potential targets for drug discovery and diagnostics. Here, we review the current status of these technologies and highlight some studies in which they have been applied in concert to the analysis of biopsy specimens.