The Rab5 Guanine Nucleotide Exchange Factor Rabex-5 Binds Ubiquitin (Ub) and Functions as a Ub Ligase through an Atypical Ub-interacting Motif and a Zinc Finger Domain

• Published in: The Journal of biological chemistry Vol. 281; no. 10; pp. 6874 - 6883
• Main Authors: Mattera, Rafael, Tsai, Yien Che, Weissman, Allan M., Bonifacino, Juan S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.03.2006; American Society for Biochemistry and Molecular Biology
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; Amino Acid Motifs; Animals; Cattle; Guanine Nucleotide Exchange Factors - metabolism; Guanine Nucleotide Exchange Factors - physiology; HeLa Cells; Humans; Protein Interaction Mapping; Protein Structure, Tertiary; rab GTP-Binding Proteins - metabolism; Two-Hybrid System Techniques; Ubiquitin - metabolism; Ubiquitin-Protein Ligases - metabolism; Ubiquitin-Protein Ligases - physiology; Zinc Fingers - physiology

Rabex-5, the mammalian orthologue of yeast Vps9p, is a guanine nucleotide exchange factor for Rab5. Rabex-5 forms a tight complex with Rabaptin-5, a multivalent adaptor protein that also binds to Rab4, Rab5, and to domains present in γ-adaptins and the Golgi-localized, γ-ear-containing, ARF-binding...