Improvement of Neutral Lipid and Polyunsaturated Fatty Acid Biosynthesis by Overexpressing a Type 2 Diacylglycerol Acyltransferase in Marine Diatom Phaeodactylum tricornutum
Microalgae have been emerging as an important source for the production of bioactive compounds. Marine diatoms can store high amounts of lipid and grow quite quickly. However, the genetic and biochemical characteristics of fatty acid biosynthesis in diatoms remain unclear. Glycerophospholipids are i...
Saved in:
| Published in | Marine drugs Vol. 11; no. 11; pp. 4558 - 4569 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Switzerland
MDPI AG
13.11.2013
MDPI |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Microalgae have been emerging as an important source for the production of bioactive compounds. Marine diatoms can store high amounts of lipid and grow quite quickly. However, the genetic and biochemical characteristics of fatty acid biosynthesis in diatoms remain unclear. Glycerophospholipids are integral as structural and functional components of cellular membranes, as well as precursors of various lipid mediators. In addition, diacylglycerol acyltransferase (DGAT) is a key enzyme that catalyzes the last step of triacylglyceride (TAG) biosynthesis. However, a comprehensive sequence-structure and functional analysis of DGAT in diatoms is lacking. In this study, an isoform of diacylglycerol acyltransferase type 2 of the marine diatom Phaeodactylum tricornutum was characterized. Surprisingly, DGAT2 overexpression in P. tricornutum stimulated more oil bodies, and the neutral lipid content increased by 35%. The fatty acid composition showed a significant increase in the proportion of polyunsaturated fatty acids; in particular, EPA was increased by 76.2%. Moreover, the growth rate of transgenic microalgae remained similar, thereby maintaining a high biomass. Our results suggest that increased DGAT2 expression could alter fatty acid profile in the diatom, and the results thus represent a valuable strategy for polyunsaturated fatty acid production by genetic manipulation. |
|---|---|
| AbstractList | Microalgae have been emerging as an important source for the production of bioactive compounds. Marine diatoms can store high amounts of lipid and grow quite quickly. However, the genetic and biochemical characteristics of fatty acid biosynthesis in diatoms remain unclear. Glycerophospholipids are integral as structural and functional components of cellular membranes, as well as precursors of various lipid mediators. In addition, diacylglycerol acyltransferase (DGAT) is a key enzyme that catalyzes the last step of triacylglyceride (TAG) biosynthesis. However, a comprehensive sequence-structure and functional analysis of DGAT in diatoms is lacking. In this study, an isoform of diacylglycerol acyltransferase type 2 of the marine diatom Phaeodactylum tricornutum was characterized. Surprisingly, DGAT2 overexpression in P. tricornutum stimulated more oil bodies, and the neutral lipid content increased by 35%. The fatty acid composition showed a significant increase in the proportion of polyunsaturated fatty acids; in particular, EPA was increased by 76.2%. Moreover, the growth rate of transgenic microalgae remained similar, thereby maintaining a high biomass. Our results suggest that increased DGAT2 expression could alter fatty acid profile in the diatom, and the results thus represent a valuable strategy for polyunsaturated fatty acid production by genetic manipulation. Microalgae have been emerging as an important source for the production of bioactive compounds. Marine diatoms can store high amounts of lipid and grow quite quickly. However, the genetic and biochemical characteristics of fatty acid biosynthesis in diatoms remain unclear. Glycerophospholipids are integral as structural and functional components of cellular membranes, as well as precursors of various lipid mediators. In addition, diacylglycerol acyltransferase (DGAT) is a key enzyme that catalyzes the last step of triacylglyceride (TAG) biosynthesis. However, a comprehensive sequence-structure and functional analysis of DGAT in diatoms is lacking. In this study, an isoform of diacylglycerol acyltransferase type 2 of the marine diatom Phaeodactylum tricornutum was characterized. Surprisingly, DGAT2 overexpression in P. tricornutum stimulated more oil bodies, and the neutral lipid content increased by 35%. The fatty acid composition showed a significant increase in the proportion of polyunsaturated fatty acids; in particular, EPA was increased by 76.2%. Moreover, the growth rate of transgenic microalgae remained similar, thereby maintaining a high biomass. Our results suggest that increased DGAT2 expression could alter fatty acid profile in the diatom, and the results thus represent a valuable strategy for polyunsaturated fatty acid production by genetic manipulation. Microalgae have been emerging as an important source for the production of bioactive compounds. Marine diatoms can store high amounts of lipid and grow quite quickly. However, the genetic and biochemical characteristics of fatty acid biosynthesis in diatoms remain unclear. Glycerophospholipids are integral as structural and functional components of cellular membranes, as well as precursors of various lipid mediators. In addition, diacylglycerol acyltransferase (DGAT) is a key enzyme that catalyzes the last step of triacylglyceride (TAG) biosynthesis. However, a comprehensive sequence-structure and functional analysis of DGAT in diatoms is lacking. In this study, an isoform of diacylglycerol acyltransferase type 2 of the marine diatom Phaeodactylum tricornutum was characterized. Surprisingly, DGAT2 overexpression in P. tricornutum stimulated more oil bodies, and the neutral lipid content increased by 35%. The fatty acid composition showed a significant increase in the proportion of polyunsaturated fatty acids; in particular, EPA was increased by 76.2%. Moreover, the growth rate of transgenic microalgae remained similar, thereby maintaining a high biomass. Our results suggest that increased DGAT2 expression could alter fatty acid profile in the diatom, and the results thus represent a valuable strategy for polyunsaturated fatty acid production by genetic manipulation.Microalgae have been emerging as an important source for the production of bioactive compounds. Marine diatoms can store high amounts of lipid and grow quite quickly. However, the genetic and biochemical characteristics of fatty acid biosynthesis in diatoms remain unclear. Glycerophospholipids are integral as structural and functional components of cellular membranes, as well as precursors of various lipid mediators. In addition, diacylglycerol acyltransferase (DGAT) is a key enzyme that catalyzes the last step of triacylglyceride (TAG) biosynthesis. However, a comprehensive sequence-structure and functional analysis of DGAT in diatoms is lacking. In this study, an isoform of diacylglycerol acyltransferase type 2 of the marine diatom Phaeodactylum tricornutum was characterized. Surprisingly, DGAT2 overexpression in P. tricornutum stimulated more oil bodies, and the neutral lipid content increased by 35%. The fatty acid composition showed a significant increase in the proportion of polyunsaturated fatty acids; in particular, EPA was increased by 76.2%. Moreover, the growth rate of transgenic microalgae remained similar, thereby maintaining a high biomass. Our results suggest that increased DGAT2 expression could alter fatty acid profile in the diatom, and the results thus represent a valuable strategy for polyunsaturated fatty acid production by genetic manipulation. |
| Author | Yang, Wei-Dong Bai, Wei-Bin Zhang, Meng-Han Niu, Ying-Fang Li, Da-Wei Li, Hong-Ye Liu, Jie-Sheng |
| AuthorAffiliation | Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China; E-Mails: 442359764@qq.com (Y.-F.N.); 756057321@qq.com (M.-H.Z.); 27432133@qq.com (D.-W.L.); tywd@jnu.edu.cn (W.-D.Y.); ljs@jnu.edu.cn (J.-S.L.); baiweibin@163.com (W.-B.B.) |
| AuthorAffiliation_xml | – name: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China; E-Mails: 442359764@qq.com (Y.-F.N.); 756057321@qq.com (M.-H.Z.); 27432133@qq.com (D.-W.L.); tywd@jnu.edu.cn (W.-D.Y.); ljs@jnu.edu.cn (J.-S.L.); baiweibin@163.com (W.-B.B.) |
| Author_xml | – sequence: 1 givenname: Ying-Fang surname: Niu fullname: Niu, Ying-Fang – sequence: 2 givenname: Meng-Han surname: Zhang fullname: Zhang, Meng-Han – sequence: 3 givenname: Da-Wei surname: Li fullname: Li, Da-Wei – sequence: 4 givenname: Wei-Dong surname: Yang fullname: Yang, Wei-Dong – sequence: 5 givenname: Jie-Sheng surname: Liu fullname: Liu, Jie-Sheng – sequence: 6 givenname: Wei-Bin surname: Bai fullname: Bai, Wei-Bin – sequence: 7 givenname: Hong-Ye surname: Li fullname: Li, Hong-Ye |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24232669$$D View this record in MEDLINE/PubMed |
| BookMark | eNptksFuEzEQhleoiLaBCw-ALHFBSIG1vd6NL0ilUIgUaA_lvJrYs4kjr53a3oh9KN4Rh5TSVvhie_zN7_HvOS2OnHdYFC9p-Y5zWb7vNc2jEmL2pDihdV1Oc7g5urc-Lk5j3JQlFzNZPSuOWcU4q2t5Uvya99vgd9ijS8R35DsOKYAlC7M1moDT5MrbcXAR0hAgoSYXkNJIzlQ-_mh8HF1aYzSRLEdyucOAP7cBYzRuRYBcj1skjHwyoEa7sqPC4G3OHW2-xMUOA0QkxpFvEIzDPZh8T67WgF6DSqMdepKCUT64IQ398-JpBzbii9t5Uvy4-Hx9_nW6uPwyPz9bTJXgszTVDJmulkixrhophFo2lLIaWCk500xoAN1VTHZlpaDkvGY0b3THs0EdV4xPivlBV3vYtNtgeghj68G0fwI-rFoIySiLLWUdMEZpMwNaKaGl1EtZyoY3ICrZVVnrw0FrOyx71CobnQ1-IPrwxJl1u_K7ls8EbyqRBd7cCgR_M2BMbW-iQmvBoR9im39eUkE55Rl9_Qjd-CG4bFVLBRd1LWRugknx6n5Fd6X87YoMvD0AKvgYA3Z3CC3bfcu1_1ouw-UjWJkEyfj9a4z9X8pvXQfblA |
| CitedBy_id | crossref_primary_10_1016_j_jbiosc_2018_03_003 crossref_primary_10_1016_j_rser_2024_114328 crossref_primary_10_1007_s10811_017_1364_3 crossref_primary_10_2139_ssrn_4104119 crossref_primary_10_1016_j_biotechadv_2021_107836 crossref_primary_10_3390_ijms242316624 crossref_primary_10_1016_j_biotechadv_2020_107554 crossref_primary_10_1007_s40518_014_0011_8 crossref_primary_10_1007_s11274_016_2164_7 crossref_primary_10_1016_j_scitotenv_2021_152895 crossref_primary_10_1016_j_fuel_2021_122738 crossref_primary_10_1080_15435075_2021_1983726 crossref_primary_10_1080_17597269_2019_1583714 crossref_primary_10_1016_j_pecs_2023_101071 crossref_primary_10_1016_j_jbiotec_2022_06_002 crossref_primary_10_1002_jobm_202100417 crossref_primary_10_1016_j_algal_2016_02_004 crossref_primary_10_1016_j_biortech_2019_121894 crossref_primary_10_1016_j_plipres_2018_07_001 crossref_primary_10_1002_lipd_12081 crossref_primary_10_1016_j_biortech_2019_121890 crossref_primary_10_1016_j_btre_2021_e00695 crossref_primary_10_1371_journal_pone_0194834 crossref_primary_10_1016_j_plipres_2014_07_001 crossref_primary_10_1073_pnas_1419818112 crossref_primary_10_3389_fmicb_2016_00432 crossref_primary_10_1098_rstb_2016_0407 crossref_primary_10_1007_s10811_022_02833_9 crossref_primary_10_3390_md19070368 crossref_primary_10_1007_s10811_015_0634_1 crossref_primary_10_1016_j_jcou_2022_102183 crossref_primary_10_1098_rstb_2016_0410 crossref_primary_10_1016_j_biortech_2025_132250 crossref_primary_10_1098_rstb_2016_0411 crossref_primary_10_3389_fmicb_2016_00546 crossref_primary_10_1002_er_5557 crossref_primary_10_1007_s00253_016_7948_z crossref_primary_10_1080_07388551_2021_1971621 crossref_primary_10_1016_j_jclepro_2023_137526 crossref_primary_10_1371_journal_pone_0299780 crossref_primary_10_1016_j_biortech_2021_126250 crossref_primary_10_3390_en16010531 crossref_primary_10_1016_j_algal_2021_102436 crossref_primary_10_1186_s12934_017_0677_x crossref_primary_10_1186_s12870_024_05014_7 crossref_primary_10_3390_md17080459 crossref_primary_10_1080_17597269_2023_2233805 crossref_primary_10_1186_s12870_017_0995_5 crossref_primary_10_1002_clen_202100407 crossref_primary_10_1186_s13068_018_1317_3 crossref_primary_10_1016_j_jbiotec_2022_10_010 crossref_primary_10_1016_j_plipres_2022_101181 crossref_primary_10_1080_21622515_2023_2283097 crossref_primary_10_1016_j_biortech_2019_121953 crossref_primary_10_1371_journal_pone_0105834 crossref_primary_10_1016_j_algal_2023_103148 crossref_primary_10_3390_su132212374 crossref_primary_10_1016_j_plipres_2019_01_003 crossref_primary_10_4236_ajps_2020_1110118 crossref_primary_10_1128_msystems_00563_22 crossref_primary_10_1038_srep18708 crossref_primary_10_1016_j_copbio_2015_09_005 crossref_primary_10_1016_j_jbiotec_2022_07_007 crossref_primary_10_3390_agronomy14050895 crossref_primary_10_1007_s12155_022_10443_z crossref_primary_10_1016_j_jbiosc_2020_09_012 crossref_primary_10_3390_md23020086 crossref_primary_10_1016_j_fuel_2021_121169 crossref_primary_10_1016_j_algal_2023_103137 crossref_primary_10_1002_biot_201900228 crossref_primary_10_1016_j_fuel_2022_124844 crossref_primary_10_1016_j_plipres_2018_03_001 crossref_primary_10_1371_journal_pone_0146158 crossref_primary_10_1016_j_esr_2024_101634 crossref_primary_10_1016_j_rser_2021_111270 crossref_primary_10_1016_j_biortech_2021_125597 crossref_primary_10_1016_j_bcab_2024_103373 crossref_primary_10_3389_fpls_2017_01751 crossref_primary_10_1016_j_chemosphere_2021_130553 crossref_primary_10_1007_s10811_018_1623_y crossref_primary_10_1186_s13068_024_02471_y crossref_primary_10_3390_cimb46100649 crossref_primary_10_1002_biot_202100603 crossref_primary_10_1016_j_biombioe_2024_107257 crossref_primary_10_3389_fmicb_2023_1295994 crossref_primary_10_1016_j_algal_2017_05_025 crossref_primary_10_1016_j_bbalip_2018_10_004 crossref_primary_10_1007_s43393_022_00095_7 crossref_primary_10_1002_biot_201600644 crossref_primary_10_1016_j_algal_2021_102590 crossref_primary_10_1016_j_rser_2021_111609 crossref_primary_10_1007_s11101_022_09804_5 crossref_primary_10_3390_md12010153 crossref_primary_10_1016_j_jece_2023_110398 crossref_primary_10_3389_fmicb_2015_01376 crossref_primary_10_1016_j_algal_2019_101425 crossref_primary_10_3390_md17060314 crossref_primary_10_1016_j_algal_2024_103621 crossref_primary_10_1016_j_scitotenv_2022_159281 crossref_primary_10_1016_j_enconman_2022_116118 crossref_primary_10_1002_biot_201900043 crossref_primary_10_2525_ecb_54_31 crossref_primary_10_1186_s13068_019_1366_2 crossref_primary_10_1016_j_algal_2015_09_004 crossref_primary_10_1080_10826068_2018_1476886 crossref_primary_10_2139_ssrn_4113112 crossref_primary_10_3390_biotech14010006 crossref_primary_10_1186_s13068_018_1309_3 crossref_primary_10_1016_j_biortech_2024_130532 crossref_primary_10_1080_17597269_2017_1402587 crossref_primary_10_1002_aocs_12718 crossref_primary_10_1007_s00253_018_8925_5 crossref_primary_10_1016_j_tplants_2017_05_005 crossref_primary_10_1021_acssynbio_4c00683 crossref_primary_10_3390_biology10070565 crossref_primary_10_1038_s41598_019_45786_9 crossref_primary_10_1007_s13762_016_1020_5 crossref_primary_10_1038_s41598_019_44710_5 crossref_primary_10_1016_j_plipres_2017_07_002 crossref_primary_10_1016_j_jbiotec_2017_10_011 crossref_primary_10_1111_pre_12162 crossref_primary_10_1016_j_foodchem_2019_06_004 crossref_primary_10_1016_j_nbt_2015_03_016 crossref_primary_10_1016_j_tibtech_2014_07_004 crossref_primary_10_3390_md20050285 crossref_primary_10_3390_md13063672 crossref_primary_10_1111_gcbb_12771 crossref_primary_10_1002_bab_1812 crossref_primary_10_1007_s42452_025_06588_z crossref_primary_10_1016_j_tibtech_2022_09_002 crossref_primary_10_1111_pbi_12523 crossref_primary_10_1007_s10811_017_1103_9 crossref_primary_10_1016_j_biortech_2022_128457 crossref_primary_10_3390_molecules26247697 crossref_primary_10_1016_j_biotechadv_2014_10_003 crossref_primary_10_1186_s13068_021_01969_z crossref_primary_10_1016_j_biortech_2021_126529 crossref_primary_10_1016_j_jwpe_2020_101701 crossref_primary_10_1021_acs_jafc_2c03489 crossref_primary_10_1016_j_tibtech_2017_10_020 crossref_primary_10_1021_acs_jafc_2c03006 crossref_primary_10_3390_md20070440 crossref_primary_10_1021_acs_energyfuels_2c02922 crossref_primary_10_1111_brv_70006 crossref_primary_10_1186_s13068_016_0478_1 crossref_primary_10_1186_s13068_018_1029_8 crossref_primary_10_1016_j_algal_2019_101504 crossref_primary_10_1007_s10529_016_2216_y crossref_primary_10_1016_j_rser_2017_10_038 crossref_primary_10_1016_j_rser_2020_110155 crossref_primary_10_1007_s10811_021_02490_4 crossref_primary_10_1016_j_biochi_2019_07_005 crossref_primary_10_1111_jpy_13063 crossref_primary_10_1111_jpy_12513 crossref_primary_10_1038_srep25494 crossref_primary_10_1080_10408398_2018_1455030 crossref_primary_10_1093_plphys_kiad581 crossref_primary_10_7717_peerj_14576 crossref_primary_10_1007_s00343_020_9325_0 crossref_primary_10_1038_s41598_018_30254_7 crossref_primary_10_3390_biology9020038 crossref_primary_10_1186_s12934_018_0906_y crossref_primary_10_1007_s10126_018_9841_5 crossref_primary_10_1016_j_enconman_2024_119037 crossref_primary_10_1186_1754_6834_7_110 crossref_primary_10_3390_md13085334 crossref_primary_10_1007_s13131_020_1659_0 crossref_primary_10_1016_j_algal_2023_103096 crossref_primary_10_1186_s13068_014_0128_4 crossref_primary_10_3390_fuels5040049 crossref_primary_10_1080_15435075_2016_1277728 crossref_primary_10_1016_j_fuel_2022_124567 crossref_primary_10_1016_j_algal_2018_08_012 crossref_primary_10_1080_07388551_2016_1237467 crossref_primary_10_1002_biot_201500272 crossref_primary_10_3389_fbioe_2020_00914 crossref_primary_10_1111_pbi_12278 crossref_primary_10_1007_s13762_018_2098_8 crossref_primary_10_3389_fpls_2019_01573 crossref_primary_10_1016_j_ymben_2014_10_002 crossref_primary_10_1016_j_biombioe_2021_106108 crossref_primary_10_1186_s13068_017_0874_1 crossref_primary_10_3389_fmars_2017_00017 crossref_primary_10_1016_j_fuproc_2025_108199 crossref_primary_10_3934_bioeng_2017_1_151 crossref_primary_10_1007_s10811_024_03367_y crossref_primary_10_1134_S1021443722010162 crossref_primary_10_1080_07388551_2020_1805402 crossref_primary_10_1146_annurev_arplant_050718_095841 crossref_primary_10_1016_j_biotechadv_2024_108460 crossref_primary_10_1016_j_jembe_2015_05_001 crossref_primary_10_1021_acs_jafc_7b04295 crossref_primary_10_1016_j_biotechadv_2016_06_004 crossref_primary_10_1016_j_algal_2024_103497 crossref_primary_10_3389_fmars_2018_00382 crossref_primary_10_1016_j_jbiotec_2016_05_005 crossref_primary_10_1007_s00253_018_8813_z |
| Cites_doi | 10.1016/S0021-9258(19)50785-2 10.1002/pmic.201100114 10.1016/j.cmet.2007.05.005 10.1186/1754-6834-6-67 10.1111/j.1742-4658.2011.08284.x 10.1016/j.tiv.2007.11.012 10.1104/pp.110.167676 10.1016/S0021-9258(18)66142-3 10.1128/EC.00203-09 10.1016/j.jbiotec.2012.04.006 10.1016/0003-2697(87)90021-2 10.1016/j.plaphy.2010.03.008 10.1126/science.1101156 10.1111/j.1467-7652.2009.00458.x 10.1128/MCB.25.12.5146-5157.2005 10.1007/s11745-006-5057-y 10.1128/EC.00364-09 10.2144/000113881 10.1016/j.jbiotec.2012.05.009 10.1016/j.gene.2007.05.022 10.1016/j.febslet.2013.01.015 10.1104/pp.126.2.861 10.1016/j.cub.2010.02.024 10.1111/j.1467-7652.2008.00358.x 10.1111/febs.12537 10.1016/j.ymben.2010.10.003 |
| ContentType | Journal Article |
| Copyright | Copyright MDPI AG 2013 2013 by the authors; licensee MDPI, Basel, Switzerland. 2013 |
| Copyright_xml | – notice: Copyright MDPI AG 2013 – notice: 2013 by the authors; licensee MDPI, Basel, Switzerland. 2013 |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7T7 7TN 7X7 7XB 88E 8FD 8FE 8FH 8FI 8FJ 8FK ABUWG AEUYN AFKRA AZQEC BBNVY BENPR BHPHI BKSAR C1K CCPQU DWQXO F1W FR3 FYUFA GHDGH GNUQQ H95 H99 HCIFZ K9. L.F L.G LK8 M0S M1P M7P P64 PCBAR PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS 7X8 5PM DOA |
| DOI | 10.3390/md11114558 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Industrial and Applied Microbiology Abstracts (Microbiology A) Oceanic Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Technology Research Database ProQuest SciTech Collection ProQuest Natural Science Journals Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection Earth, Atmospheric & Aquatic Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Korea ASFA: Aquatic Sciences and Fisheries Abstracts Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources ASFA: Marine Biotechnology Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts Aquatic Science & Fisheries Abstracts (ASFA) Professional Biological Sciences ProQuest Health & Medical Collection PML(ProQuest Medical Library) Biological Science Database Biotechnology and BioEngineering Abstracts Earth, Atmospheric & Aquatic Science Database ProQuest Central Premium ProQuest One Academic ProQuest - Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database Aquatic Science & Fisheries Abstracts (ASFA) Professional ProQuest Central Student Technology Research Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest Central Earth, Atmospheric & Aquatic Science Collection ProQuest One Applied & Life Sciences ProQuest One Sustainability ProQuest Health & Medical Research Collection Health Research Premium Collection Oceanic Abstracts Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources Industrial and Applied Microbiology Abstracts (Microbiology A) ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Earth, Atmospheric & Aquatic Science Database ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ASFA: Aquatic Sciences and Fisheries Abstracts Engineering Research Database ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE Publicly Available Content Database MEDLINE - Academic CrossRef |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Open Access Full Text url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Pharmacy, Therapeutics, & Pharmacology |
| EISSN | 1660-3397 |
| EndPage | 4569 |
| ExternalDocumentID | oai_doaj_org_article_12fa221178a14c5d99db909737a549f4 PMC3853745 3334859031 24232669 10_3390_md11114558 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- 29M 2WC 53G 5GY 5VS 7X7 88E 8CJ 8FE 8FH 8FI 8FJ AADQD AAHBH AAYXX ABUWG ACGFO ACIHN ACPRK ADBBV ADRAZ AEAQA AENEX AEUYN AFKRA AFRAH AFZYC AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BBNVY BCNDV BENPR BHPHI BKSAR BPHCQ BVXVI CCPQU CITATION CS3 D1J DIK DU5 E3Z EBD EDH F5P FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IPNFZ KQ8 LK5 LK8 M1P M48 M7P M7R MK0 MODMG M~E O5R O5S OK1 OVT P2P PCBAR PGMZT PHGZM PHGZT PIMPY PQQKQ PROAC PSQYO RIG RNS RPM TR2 UKHRP XSB CGR CUY CVF ECM EIF NPM PJZUB PPXIY PQGLB 3V. 7T7 7TN 7XB 8FD 8FK AZQEC C1K DWQXO F1W FR3 GNUQQ H95 H99 K9. L.F L.G P64 PKEHL PQEST PQUKI PRINS 7X8 5PM PUEGO |
| ID | FETCH-LOGICAL-c538t-d2e2d4be1e647955cb71126a20932d25daadf429f04ca03362129fdf3035f3c23 |
| IEDL.DBID | M48 |
| ISSN | 1660-3397 |
| IngestDate | Wed Aug 27 01:22:35 EDT 2025 Thu Aug 21 17:24:15 EDT 2025 Thu Jul 10 18:07:30 EDT 2025 Fri Jul 25 10:39:59 EDT 2025 Mon Jul 21 06:02:22 EDT 2025 Tue Jul 01 03:19:23 EDT 2025 Thu Apr 24 22:55:08 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 11 |
| Language | English |
| License | https://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c538t-d2e2d4be1e647955cb71126a20932d25daadf429f04ca03362129fdf3035f3c23 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.3390/md11114558 |
| PMID | 24232669 |
| PQID | 1535665900 |
| PQPubID | 2032365 |
| PageCount | 12 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_12fa221178a14c5d99db909737a549f4 pubmedcentral_primary_oai_pubmedcentral_nih_gov_3853745 proquest_miscellaneous_1459151313 proquest_journals_1535665900 pubmed_primary_24232669 crossref_primary_10_3390_md11114558 crossref_citationtrail_10_3390_md11114558 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 20131113 |
| PublicationDateYYYYMMDD | 2013-11-13 |
| PublicationDate_xml | – month: 11 year: 2013 text: 20131113 day: 13 |
| PublicationDecade | 2010 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland – name: Basel |
| PublicationTitle | Marine drugs |
| PublicationTitleAlternate | Mar Drugs |
| PublicationYear | 2013 |
| Publisher | MDPI AG MDPI |
| Publisher_xml | – name: MDPI AG – name: MDPI |
| References | Wagner (ref_8) 2010; 48 Radakovits (ref_16) 2010; 9 Radakovits (ref_19) 2011; 13 Armbrust (ref_21) 2004; 306 Williams (ref_15) 2010; 20 Yang (ref_25) 2013; 6 ref_12 Jako (ref_4) 2001; 126 Andrianov (ref_17) 2010; 8 Xu (ref_6) 2008; 6 Lung (ref_3) 2006; 41 Macedo (ref_24) 2008; 22 Oakes (ref_5) 2011; 155 Nguyen (ref_10) 2011; 11 Kennedy (ref_2) 1956; 222 Leu (ref_13) 2011; 278 Moellering (ref_20) 2010; 9 ref_23 Gong (ref_14) 2013; 587 Chen (ref_11) 2012; 162 Kornberg (ref_1) 1953; 204 Siaut (ref_22) 2007; 406 Monetti (ref_7) 2007; 6 Park (ref_18) 2005; 25 Bogen (ref_9) 2012; 162 Chomczynski (ref_26) 1987; 162 |
| References_xml | – volume: 222 start-page: 193 year: 1956 ident: ref_2 article-title: The function of cytidine coenzymes in the biosynthesis of phospholipides publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)50785-2 – volume: 11 start-page: 4266 year: 2011 ident: ref_10 article-title: Proteomic profiling of oil bodies isolated from the unicellular green microalga Chlamydomonas reinhardtii: With focus on proteins involved in lipid metabolism publication-title: Proteomics doi: 10.1002/pmic.201100114 – volume: 6 start-page: 69 year: 2007 ident: ref_7 article-title: Dissociation of hepatic steatosis and insulin resistance in mice overexpressing DGAT in the liver publication-title: Cell Metab. doi: 10.1016/j.cmet.2007.05.005 – volume: 6 start-page: 67 year: 2013 ident: ref_25 article-title: Molecular and cellular mechanisms of neutral lipid accumulation in diatom following nitrogen deprivation publication-title: Biotechnol. Biofuels doi: 10.1186/1754-6834-6-67 – volume: 278 start-page: 3651 year: 2011 ident: ref_13 article-title: Cloning and molecular characterization of a novel acyl-CoA: Diacylglycerol acyltransferase 1-like gene (PtDGAT1) from the diatom Phaeodactylum tricornutum publication-title: FEBS J. doi: 10.1111/j.1742-4658.2011.08284.x – volume: 22 start-page: 716 year: 2008 ident: ref_24 article-title: Effects of herbicide bentazon on growth and photosystem II maximum quantum yield of the marine diatom Skeletonema costatum publication-title: Toxicol. in Vitro doi: 10.1016/j.tiv.2007.11.012 – volume: 155 start-page: 1146 year: 2011 ident: ref_5 article-title: Expression of fungal diacylglycerol acyltransferase2 genes to increase kernel oil in maize publication-title: Plant Physiol. doi: 10.1104/pp.110.167676 – volume: 204 start-page: 329 year: 1953 ident: ref_1 article-title: Enzymatic esterification of α-glycerophosphate by long chain fatty acids publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)66142-3 – volume: 9 start-page: 97 year: 2010 ident: ref_20 article-title: RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii publication-title: Eukaryot. Cell doi: 10.1128/EC.00203-09 – volume: 162 start-page: 13 year: 2012 ident: ref_9 article-title: Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii publication-title: J. Biotechnol. doi: 10.1016/j.jbiotec.2012.04.006 – volume: 162 start-page: 156 year: 1987 ident: ref_26 article-title: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction publication-title: Anal. Biochem. doi: 10.1016/0003-2697(87)90021-2 – volume: 48 start-page: 407 year: 2010 ident: ref_8 article-title: Identification and characterization of an acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) gene from the microalga O. tauri publication-title: Plant Physiol. Biochem. doi: 10.1016/j.plaphy.2010.03.008 – volume: 306 start-page: 79 year: 2004 ident: ref_21 article-title: The genome of the diatom Thalassiosira pseudonana: Ecology, evolution, and metabolism publication-title: Science doi: 10.1126/science.1101156 – volume: 8 start-page: 277 year: 2010 ident: ref_17 article-title: Tobacco as a production platform for biofuel: Overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2009.00458.x – volume: 25 start-page: 5146 year: 2005 ident: ref_18 article-title: Overexpression of glucose-6-phosphate dehydrogenase is associated with lipid dysregulation and insulin resistance in obesity publication-title: Mol. Cell Biol. doi: 10.1128/MCB.25.12.5146-5157.2005 – volume: 41 start-page: 1073 year: 2006 ident: ref_3 article-title: Diacylglycerol acyltransferase: A key mediator of plant triacylglycerol synthesis publication-title: Lipids doi: 10.1007/s11745-006-5057-y – volume: 9 start-page: 486 year: 2010 ident: ref_16 article-title: Genetic engineering of algae for enhanced biofuel production publication-title: Eukaryot. Cell doi: 10.1128/EC.00364-09 – ident: ref_23 doi: 10.2144/000113881 – volume: 162 start-page: 28 year: 2012 ident: ref_11 article-title: A look at diacylglycerol acyltransferases (DGATs) in algae publication-title: J. Biotechnol. doi: 10.1016/j.jbiotec.2012.05.009 – volume: 406 start-page: 23 year: 2007 ident: ref_22 article-title: Molecular toolbox for studying diatom biology in Phaeodactylum tricornutum publication-title: Gene doi: 10.1016/j.gene.2007.05.022 – volume: 587 start-page: 481 year: 2013 ident: ref_14 article-title: Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-Coenzyme A: Diacylglycerol acyltransferase family in the diatom Phaeodactylum tricornutum publication-title: FEBS Lett. doi: 10.1016/j.febslet.2013.01.015 – volume: 126 start-page: 861 year: 2001 ident: ref_4 article-title: Seed-specific over-expression of an arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight publication-title: Plant Physiol. doi: 10.1104/pp.126.2.861 – volume: 20 start-page: R219 year: 2010 ident: ref_15 article-title: New biofuel questions publication-title: Curr. Biol. doi: 10.1016/j.cub.2010.02.024 – volume: 6 start-page: 799 year: 2008 ident: ref_6 article-title: Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2008.00358.x – ident: ref_12 doi: 10.1111/febs.12537 – volume: 13 start-page: 89 year: 2011 ident: ref_19 article-title: Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum publication-title: Metab. Eng. doi: 10.1016/j.ymben.2010.10.003 |
| SSID | ssj0035894 |
| Score | 2.48503 |
| Snippet | Microalgae have been emerging as an important source for the production of bioactive compounds. Marine diatoms can store high amounts of lipid and grow quite... |
| SourceID | doaj pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 4558 |
| SubjectTerms | Acid production Amino Acid Sequence Bioactive compounds Biochemistry Biosynthesis Cloning, Molecular - methods diacylglycerol acyltranferase Diacylglycerol O-Acyltransferase - genetics diatom Diatoms - enzymology Diatoms - genetics Diatoms - metabolism Fatty Acids - genetics Fatty Acids - metabolism Fatty Acids, Unsaturated - biosynthesis Lipids - biosynthesis Microalgae Molecular Sequence Data neutral lipid Photosynthesis - genetics Polyunsaturated fatty acids PUFA |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1La9tAEF5KTr2UvqsmLVNaAoWISqtdPY5JWxMKSX1IIDex2kdqcFbFkqD6Uf2PnZHkVwn00pvtHfDIM5rvm_XqG8Y-KC0yYwoZ5jrKQ-T_MizyqAql4xnCu44rRxv6F5fp-bX4diNvdkZ90ZmwUR54_OE-xdwpjl1KlqtYaGmKwlQFacxkClsbNyiBIuatm6mxBicyH0YgxmmKVQYhdxQmxVcRNvpUJYSkIe87UDQo9t9HM_8-LbkDP7PH7NHEG-F09PcJe2D9U3Y8H4Wn-xO42j5H1ZzAMcy3ktT9M_Z73DsYtgKhdnCJhvglQJOrDShvYF4v-843JPOJ7NPATLVtD6cal88WddN7JIrNooGqh--Y_fbXeIDW34IC6mWBw5cFerK8Xfbarmp0Fd-0Ay1GzxoLCw8Xip41JMO2viMXbW2UbnsskECTAuqV79ru7jm7nn29-nweToMaQo31sg0Nt9yIysY2FVkhpa4ozqnCwCTccGmUMg6Bz0VCqyhBzESW4YxD-JQu0Tx5wQ587e0rBlkaqUhGmueREiKzOXZURrvKikwg87MB-7iOWaknFXMaprEssZuh-Jbb-Abs_cb256jdca_VGYV-Y0F628MHmIXllIXlv7IwYEfrxCmnItCUCCZIlmksa8DebZbx9qX_ZJS3dYc2QhZIupI4CdjLMc82nhDVRf5UBCzby8A9V_dX_OLHIBGeIAvLhHz9P67tkD3kNAOEzj4mR-ygXXX2DTKxtno73HR_AE2CMuk priority: 102 providerName: Directory of Open Access Journals – databaseName: Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1La9tAEF7a9NJL6btq0rKlJVCIqLTa9UqnkrQ1oZDUhwR8E6t9OAZnN7FkqH5U_2NnJNmOS-jR3gENzOub1egbQj4pzaUxhYhzneQx4H8RF3lSxcIxCeVdp5XDC_2z89HpJf85FdPhwq0exirXObFL1CZovCP_ApEJyAN3XH69uY1xaxS-XR1WaDwkj5C6DEe65HTTcGUiL3hPSZpBaw8tPuYHLnC9-50i1HH13wcw_52TvFN4xk_JkwEx0uPexM_IA-ufk8NJTzndHtGL7RdU9RE9pJMtGXX7gvzpbw26S0AaHD0HQXgIxZ3Vhipv6CQs2pWvkeATcKehY9U0LT3WcHwyD3XrASLW85pWLf0Ffm9_96OzfkYVxS6WMvp9DposZotW22UAVeFH0wFi0Ky2dO7pmcKvDFGwCdeoog1G6aaF1EhxR0BY-lWzun5JLsc_Lr6dxsOKhlhDpmxiwywzvLKpHXFZCKErtPBIsQRwoWHCKGUclDyXcK2SDKol4AtnHBRO4TLNsldkzwdv3xAqR4lKRKJZnijOpc2hlzLaVZZLDpjPRuTz2malHvjLcY3GooQ-Bu1bbu0bkY8b2ZueteNeqRM0_UYCmba7P8JyVg6BW6bMKQZdssxVyrUwRWGqAjmOpILW2vGIHKwdpxzCvy63zhqRD5tjCFx8G6O8DSuQ4aIAuJWlWURe93620QRBLiCnIiJyxwN3VN098fOrjhw8A_wluXj7f7X2yWOGez1wnjE7IHvNcmXfAbpqqvddCP0F4ykpgw priority: 102 providerName: ProQuest |
| Title | Improvement of Neutral Lipid and Polyunsaturated Fatty Acid Biosynthesis by Overexpressing a Type 2 Diacylglycerol Acyltransferase in Marine Diatom Phaeodactylum tricornutum |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/24232669 https://www.proquest.com/docview/1535665900 https://www.proquest.com/docview/1459151313 https://pubmed.ncbi.nlm.nih.gov/PMC3853745 https://doaj.org/article/12fa221178a14c5d99db909737a549f4 |
| Volume | 11 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1La9tAEF7yuPRS-q7a1GxpCRSiIq92vdKhlLiNCQW7psSQm1jtwzE4q9aSIfpR_Y-dkfyIg089SjtCg2ZG880-viHko9JcGpOKMNFREgL-F2GaRHkoHJOQ3nU3dzihPxz1Lif8x7W4PiDr_p2rD1juLe2wn9RkMf9896f-CgH_BStOKNmhdMe450Ikh-QYMpLEAB3yzWpCLJKUt9SkD-SRChhXKnu43_leXmro-_dhzodbJ-_losET8ngFIul5a_Wn5MD6Z-R03LJQ12f0anuoqjyjp3S85aeun5O_7URCMy9IC0dHIAgvodjG2lDlDR0X83rpS-T8BChq6EBVVU3PNQz3Z0VZe0CN5aykeU1_QijYu3Y3rZ9SRbGwpYx-n4Em8-m81nZRgKpwUTUYGTQrLZ15OlR48BAFq-IWVbSFUbqq4W9JsW1AsfDLann7gkwGF1ffLsNV14ZQw8-zCg2zzPDcdm2Py1QInaPRe4pFABUNE0Yp4yALuohrFcWQQAFyOOMglwoXaxa_JEe-8PY1obIXqUhEmiWR4lzaBMoro11uueRgNhuQT2ubZXpFaY6dNeYZlDZo6mxr6oB82Mj-bok89kr10fQbCSTfbm4Ui2m2iuWsy5xiUDjLRHW5FiZNTZ4i7ZFUUG07HpCTteNka4fOILMAcsYerQF5vxmGWMYFGuVtsQQZLlJAYHE3Dsir1s82mqz9NCByxwN3VN0d8bObhi88BkgmuXjz30--JY8YdgHB3Y_xCTmqFkv7DrBYlXfIobyWHXLcvxiNf3WaGY1OE3z_AHQSPBY |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3fb9MwELam7gFeEL8JG2AETEJatNSxm-QBoZWt6thaKtRJe8sc2-kqtcloUkH-KPgbucuPdkUTb3tMfFJOOt_n7xz7PkLeS8U9rQNh-8rxbeD_wg58J7JFzDxY3lU7inFDfzDs9M_51wtxsUX-NHdh8Fhlg4klUOtU4R75AWQmMA_UuPx8_cNG1Sj8u9pIaFTT4tQUP6Fkyz6dHEF8PzDWOx5_6du1qoCtILlzWzPDNI9M23S4FwihInSqIxnU9kwzoaXUMaB07HAlHRcAHpbEWMeA9SJ2FTY6AMjf5i6UMi2y3T0ejr432O8KP-BVE1TXDZyDuUZE4gIF5W8se6U6wG2U9t-TmTeWut5D8qDmqPSwmlSPyJZJHpO9UdXkutin4_WdrWyf7tHRuv118YT8rvYpym1HmsZ0CIbwEYoq2ZrKRNNROiuWSYYtRYHpatqTeV7QQwXD3WmaFQmQ0mya0aig3yDTzK_qsG4yoZJi3UwZPZqCJ7PJrFBmkYKr8JCXFBw8ywydJnQg8V4jGubpHF00qZYqLwCMKaoSpItkmS_nT8n5nYTvGWklaWJeEOp1HOkIRzHfkZx7xofqTas4MtzjwDKNRT42MQtV3TEdhTtmIVROGN9wHV-LvFvZXld9Qm616mLoVxbY27t8kS4mYQ0VYZvFkkFd7vmyzZXQQaCjALsqeRKK-ZhbZLeZOGENOFm4Tg-LvF0NA1Tg_x-ZmHQJNlwEQPDctmuR59U8W3mCtBq4WmARb2MGbri6OZJMr8p25C4wPo-Ll_936w251x8PzsKzk-HpDrnPUFUET1O6u6SVL5bmFXC7PHpdJxQll3edw38BXTtm9g |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEF5VRUJcEG8MBRYBlZBqxV7vZu0DQi0haikNObRSb-56HyFSYpfYEfhH8Qf4dczYeTSo4tajvSN5pJn59pv17Awhb5Xm0phE-LEOYh_4v_CTOMh84ZiE7V2HmcMD_ZNB9_CMfzkX51vkz_IuDJZVLjGxAWpTaDwj70BkAvPAGZcdtyiLGPb6Hy9_-DhBCv-0LsdptC5ybOufkL6VH456YOt3jPU_n3469BcTBnwNgV75hllmeGZD2-UyEUJnqGBXMcjzmWHCKGUcILYLuFZBBGAP26MzDnBfuEhj0wOA_1syEiHGmDxfJXuRiBPetkONoiToTA1iExc4Wv7KBtjMCbiO3P5bo3ll0-vfI3cXbJXut-51n2zZ_AHZHbbtrus9erq-vVXu0V06XDfCrh-S3-2JRXMASQtHByAIH6E4L9tQlRs6LCb1PC-xuShwXkP7qqpquq9h-WBclHUO9LQclzSr6TeIOfurLdvNR1RRzKApo70xaDIZTWptZwWoCg9VQ8ZBs9LScU5PFN5wRMGqmKKKtjBKVzXAMsX5BMUsn1fz6SNydiPGe0y28yK3TwmV3UAFItAsDhTn0saQxxntMsslB75pPfJ-abNUL3qn4wiPSQo5FNo3XdvXI29Wspdtx5BrpQ7Q9CsJ7PLdvChmo3QBGmnInGKQoctYhVwLkyQmS7C_klSQ1jvukZ2l46QL6CnTdaB45PVqGUAD_wSp3BZzkOEiAaoXhZFHnrR-ttIECTawtsQjcsMDN1TdXMnH35vG5BFwP8nFs_-r9YrchshNvx4Njp-TOwzHi2BZZbRDtqvZ3L4AkldlL5toouTipsP3L8cCacY |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Improvement+of+Neutral+Lipid+and+Polyunsaturated+Fatty+Acid+Biosynthesis+by+Overexpressing+a+Type+2+Diacylglycerol+Acyltransferase+in+Marine+Diatom+Phaeodactylum+tricornutum&rft.jtitle=Marine+drugs&rft.au=Niu%2C+Ying-Fang&rft.au=Zhang%2C+Meng-Han&rft.au=Li%2C+Da-Wei&rft.au=Yang%2C+Wei-Dong&rft.date=2013-11-13&rft.pub=MDPI&rft.eissn=1660-3397&rft.volume=11&rft.issue=11&rft.spage=4558&rft.epage=4569&rft_id=info:doi/10.3390%2Fmd11114558&rft_id=info%3Apmid%2F24232669&rft.externalDocID=PMC3853745 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1660-3397&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1660-3397&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1660-3397&client=summon |