Characterizing Regulatory and Functional Differentiation between Maize Mesophyll and Bundle Sheath Cells by Transcriptomic Analysis

• Published in: Plant physiology (Bethesda) Vol. 160; no. 1; pp. 165 - 177
• Main Authors: Chang, Yao-Ming, Liu, Wen-Yu, Shih, Arthur Chun-Chieh, Shen, Meng-Ni, Lu, Chen-Hua, Lu, Mei-Yeh Jade, Yang, Hui-Wen, Wang, Tzi-Yuan, Chen, Sean C.-C., Chen, Stella Maris, Li, Wen-Hsiung, Ku, Maurice S.B.
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01.09.2012
• Subjects: Biological and medical sciences; bundle sheath cells; C4 plants; carbon; Cell Differentiation; cell division; Cell Wall - genetics; Cell Wall - metabolism; Cell walls; Cells; Chloroplasts; Chromosome Mapping; Corn; data collection; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genes, Plant; Genome Analysis; hydrogen; leaves; mesophyll; Mesophyll Cells - cytology; Mesophyll Cells - metabolism; metabolites; oxygen; Photosynthesis; Plant cells; Plant Cells - metabolism; Plant Epidermis - genetics; Plant Epidermis - metabolism; Plant Leaves - cytology; Plant Leaves - genetics; Plant Leaves - metabolism; Plant physiology and development; Plant Proteins - metabolism; Plant Vascular Bundle - cytology; Plant Vascular Bundle - genetics; Plant Vascular Bundle - metabolism; Plants; Plasmodesmata - genetics; Plasmodesmata - metabolism; post-translational modification; Protein Biosynthesis; protein degradation; Protein Transport; Protoplasts - cytology; Protoplasts - metabolism; RNA; RNA, Plant - analysis; RNA, Plant - genetics; sequence analysis; Signal Transduction; transcription (genetics); transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptome; Transcriptomes; transcriptomics; transporters; Zea mays; Zea mays - cytology; Zea mays - genetics; Zea mays - metabolism; Monocotyledones; Zea mays; Mesophyll; Plant physiology; Gramineae; Vascular bundle; Angiospermae; Transcriptome; Spermatophyta; Plant leaf; Cereal crop

To study the regulatory and functional differentiation between the mesophyll (M) and bundle sheath (BS) cells of maize (Zea mays), we isolated large quantities of highly homogeneous M and BS cells from newly matured second leaves for transcriptome profiling by RNA sequencing. A total of 52,421 annot...