Crystallization and preliminary X-ray diffraction studies of a deglycosylated glucose oxidase from Penicillium amagasakiense
The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. Deglycosylation and purification to isoelectric homogeneity were shown to be an important prerequisite step to obtain crystals suitable for X-ray investigation...
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| Published in | Journal of molecular biology Vol. 223; no. 4; pp. 1167 - 1169 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford
Elsevier Ltd
20.02.1992
Elsevier |
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| Abstract | The dimeric glucose oxidase from
Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. Deglycosylation and purification to isoelectric homogeneity were shown to be an important prerequisite step to obtain crystals suitable for X-ray investigations. Crystals of the deglycosylated enzyme were reproducibly grown using ammonium sulfate as precipitant at pH 7.4 to 7.5. Crystals diffract to at least 2.0 Å resolution and belong to the orthorhombic space group
P2
12
12
1, with refined lattice constants of
a = 59.3
A
̊
, b = 136.3
A
̊
and
c = 156.7
A
̊
. Assuming two monomers (≈ 135 kDa) per asymmetric unit the
V
m value is 2.3 Å
3/Da. |
|---|---|
| AbstractList | The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated purified and crystallized as a complex with its coenzyme FAD. Deglycosylation and purification to isoelectric homogeneity were shown to be an important prerequisite step to obtain crystals suitable for X-ray investigations. Crystals of the deglycosylated enzyme were reproducibly grown using ammonium sulfate as precipitant at pH 7.4 to 7.5. Crystals diffract to at least 2.0 angstroms resolution and belong to the orthorhombic space group P2(1)2(1)2(1), with refined lattice constants of a = 59.3 angstroms, b = 136.3 angstroms and c = 156.7 angstroms. Assuming two monomers (approximately 135 kDa) per asymmetric unit the Vm value is 2.3 cubic angstroms/Da. The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. Deglycosylation and purification to isoelectric homogeneity were shown to be an important prerequisite step to obtain crystals suitable for X-ray investigations. Crystals of the deglycosylated enzyme were reproducibly grown using ammonium sulfate as precipitant at pH 7.4 to 7.5. Crystals diffract to at least 2.0 A resolution and belong to the orthorhombic space group P2(1)2(1)2(1), with refined lattice constants of a = 59.3 A, b = 136.3 A and c = 156.7 A. Assuming two monomers (approximately 135 kDa) per asymmetric unit the Vm value is 2.3 A3/Da. The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. Deglycosylation and purification to isoelectric homogeneity were shown to be an important prerequisite step to obtain crystals suitable for X-ray investigations. Crystals of the deglycosylated enzyme were reproducibly grown using ammonium sulfate as precipitant at pH 7.4 to 7.5. Crystals diffract to at least 2.0 A resolution and belong to the orthorhombic space group P2(1)2(1)2(1), with refined lattice constants of a = 59.3 A, b = 136.3 A and c = 156.7 A. Assuming two monomers (approximately 135 kDa) per asymmetric unit the Vm value is 2.3 A3/Da.The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. Deglycosylation and purification to isoelectric homogeneity were shown to be an important prerequisite step to obtain crystals suitable for X-ray investigations. Crystals of the deglycosylated enzyme were reproducibly grown using ammonium sulfate as precipitant at pH 7.4 to 7.5. Crystals diffract to at least 2.0 A resolution and belong to the orthorhombic space group P2(1)2(1)2(1), with refined lattice constants of a = 59.3 A, b = 136.3 A and c = 156.7 A. Assuming two monomers (approximately 135 kDa) per asymmetric unit the Vm value is 2.3 A3/Da. The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. Deglycosylation and purification to isoelectric homogeneity were shown to be an important prerequisite step to obtain crystals suitable for X-ray investigations. Crystals of the deglycosylated enzyme were reproducibly grown using ammonium sulfate as precipitant at pH 7.4 to 7.5. Crystals diffract to at least 2.0 Å resolution and belong to the orthorhombic space group P2 12 12 1, with refined lattice constants of a = 59.3 A ̊ , b = 136.3 A ̊ and c = 156.7 A ̊ . Assuming two monomers (≈ 135 kDa) per asymmetric unit the V m value is 2.3 Å 3/Da. The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. |
| Author | Hecht, Hans-Jürgen Kalisz, Henryk M. Schmid, Rolf D. Schomburg, Dietmar Hendle, Jörg |
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| Keywords | crystallization Penicillium amagasakiense glucose oxidase Fungi Glucose oxidase Unit cell Purification Enzyme Crystallization Coenzyme enzyme complex Fungi Imperfecti X ray diffraction Thallophyta |
| Language | English |
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| Snippet | The dimeric glucose oxidase from
Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. Deglycosylation... The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated purified and crystallized as a complex with its coenzyme FAD. Deglycosylation and... The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. Deglycosylation... The dimeric glucose oxidase from Penicillium amagasakiense was deglycosylated, purified and crystallized as a complex with its coenzyme FAD. |
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| SubjectTerms | Biological and medical sciences crystal structure Crystalline structure crystallization Crystallography crystals dimensions enzymology FAD Fundamental and applied biological sciences. Psychology glucose oxidase Glucose Oxidase - ultrastructure interaction Molecular biophysics Penicillium Penicillium - enzymology Penicillium amagasakiense Protein Conformation purification Structure in molecular biology ultrastructure X-ray crystallography X-Ray Diffraction |
| Title | Crystallization and preliminary X-ray diffraction studies of a deglycosylated glucose oxidase from Penicillium amagasakiense |
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