Cell Fractionation and the Identification of Host Proteins Involved in Plant–Virus Interactions

• Published in: Pathogens (Basel) Vol. 13; no. 1; p. 53
• Main Authors: Gomaa, Amany E., El Mounadi, Kaoutar, Parperides, Eric, Garcia-Ruiz, Hernan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.01.2024
• Subjects: Analysis; Antiviral activity; Antiviral agents; antiviral properties; Cell Fractionation; Centrifugation; Chloroplasts; Cloning; Comparative analysis; Control; Crops; Disease resistance; Dosage and administration; Enzymes; Extracellular vesicles; Flowers & plants; Fractionation; genes; Genetic modification; Genome editing; Genomes; Host plants; Host-Pathogen Interactions; Hybridization; Identification and classification; Immunoprecipitation; Infections; Kinases; Localization; Mass Spectrometry; Mass spectroscopy; Mitochondria; Organelles; Pathogenesis; Plant Diseases - virology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Viruses; precipitin tests; Protein interaction; Proteins; protein–protein interactions; Proteome - metabolism; Proteomes; Proteomics; Proteomics - methods; Replication; RNA polymerase; Roles; subcellular localization; Tobacco; Viral infections; Viral Proteins - genetics; Viral Proteins - metabolism; Virus diseases of plants; Virus Replication; Viruses; virus–host interactions; Yeast; yeasts; United States; cell fractionation; protein–protein interactions; mass spectrometry; subcellular localization; virus–host interactions

Plant viruses depend on host cellular factors for their replication and movement. There are cellular proteins that change their localization and/or expression and have a proviral role or antiviral activity and interact with or target viral proteins. Identification of those proteins and their roles d...