Copper is a Cofactor of the Formylglycine‐Generating Enzyme

• Published in: Chembiochem : a European journal of chemical biology Vol. 18; no. 2; pp. 161 - 165
• Main Authors: Knop, Matthias, Dang, Thanh Quy, Jeschke, Gunnar, Seebeck, Florian P.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 17.01.2017; John Wiley and Sons Inc
• Subjects: Actinobacteria - enzymology; Biocatalysis; Catalytic Domain; Communication; Communications; copper; Copper - chemistry; Copper - metabolism; Cysteine - chemistry; Cysteine - metabolism; Electron Spin Resonance Spectroscopy; Enzymes; EPR; formylglycine; Glycine - analogs & derivatives; Glycine - chemistry; Glycine - metabolism; Humans; Kinetics; Molecular Dynamics Simulation; oxidases; oxygen activation; protein engineering; Sulfatases - chemistry; Sulfatases - metabolism; copper; protein engineering; EPR; oxygen activation; formylglycine; oxidases

Formylglycine‐generating enzyme (FGE) is an O2‐utilizing oxidase that converts specific cysteine residues of client proteins to formylglycine. We show that CuI is an integral cofactor of this enzyme and binds with high affinity (KD=of 10−17 m) to a pair of active‐site cysteines. These findings estab...