Endocytosis of HLA and H‐2 molecules on transformed murine cells measured by fluorescence dequenching of liposome‐encapsulated carboxyfluorescein

• Published in: The EMBO journal Vol. 2; no. 12; pp. 2285 - 2291
• Main Authors: Truneh, A., Machy, P., Barbet, J., Mishal, Z., Lemonnier, F.A., Leserman, L.D.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 01.01.1983
• Subjects: Animals; Antibodies, Monoclonal; Biological and medical sciences; Cell physiology; cell surface; Cell Transformation, Viral; Endocytosis; Epitopes - analysis; Fluoresceins; Fundamental and applied biological sciences. Psychology; H-2 Antigens - genetics; H-2 Antigens - immunology; HeLa Cells - metabolism; histocompatibility antigen H-2; histocompatibility antigen HLA; HLA Antigens - genetics; HLA Antigens - immunology; Humans; L cells; L Cells - immunology; Liposomes - administration & dosage; Major Histocompatibility Complex; Methotrexate - pharmacology; Mice; Molecular and cellular biology; Simian virus 40 - genetics; Spectrometry, Fluorescence; Human; HLA-System; Histocompatibility system; Transformed cell; Rodentia; Fluorescence; Liposome; Monoclonal antibody; H-2-System; Cell surface; Fluorescence spectrometry; Antigen; Proteins; Vertebrata; Endocytosis; Mammalia; Mouse; Cytofluorimetry

We have studied internalization of cell surface proteins encoded by genes of the human major histocompatibility complex (HLA) transferred into murine L cells, in comparison with mouse (H‐2) histocompatibility determinants. This internalization was measured by the use of monoclonal antibodies directe...