Effect of dihydropyridines on calcium channels in isolated smooth muscle cells from rat vena cava

• Published in: British journal of pharmacology Vol. 105; no. 2; pp. 321 - 328
• Main Authors: Mironneau, J., Yamamoto, T., Sayet, I., Arnaudeau, S., Rakotoarisoa, L., Mironneau, C.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.1992; Nature Publishing
• Subjects: Animals; Barium - metabolism; Biological and medical sciences; Ca2+ channel; calcium; Calcium Channel Blockers - pharmacology; Calcium Channels - drug effects; Calcium Channels - metabolism; Cardiovascular system; channels; dihydropyridine; dihydropyridines; Dihydropyridines - pharmacology; effects on; Electrophysiology; In Vitro Techniques; Ion Channels - drug effects; isolated smooth muscle cells; Isradipine; Medical sciences; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - drug effects; Na+ channel; Pharmacology. Drug treatments; Rats; Receptors, Nicotinic - drug effects; Receptors, Nicotinic - metabolism; smooth muscle; Sodium Channels - drug effects; Tetrodotoxin - pharmacology; Vasodilator agents. Cerebral vasodilators; Vena cava; Venae Cavae - cytology; Venae Cavae - drug effects; Vasodilator agent; Rat; Rodentia; Calcium antagonist; Electrophysiology; Smooth muscle; Ionic channel; Binding site; Muscle contraction; In vitro; Dihydropyridine derivative; Vertebrata; Mammalia; Sodium; Animal; Circulatory system; Vena cava

Summary 1 . Whole‐cell patch‐clamp method was applied to single smooth muscle cells freshly isolated from the rat inferior vena cava. 2 . Depolarizing pulses, applied from a holding potential of − 90 mV, activated both Na+ and Ca2+ channels. The fast Na+ current was inhibited by nanomolar concentrat...