Engineering of chicken avidin: a progressive series of reduced charge mutants

• Published in: FEBS letters Vol. 441; no. 2; pp. 313 - 317
• Main Authors: Marttila, Ari T, Airenne, Kari J, Laitinen, Olli H, Kulik, Tikva, Bayer, Edward A, Wilchek, Meir, Kulomaa, Markku S
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 18.12.1998
• Subjects: Animals; Avidin; Avidin - chemistry; Avidin - genetics; Avidin - metabolism; Avidin-biotin technology; Biotin - metabolism; Charge mutant; Chickens; DNA, Complementary; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Hot Temperature; Mutagenesis, Site-Directed; Protein Engineering; Spodoptera; Charge mutant; Protein engineering; Avidin-biotin technology; Avidin

Avidin, a positively charged egg-white glycoprotein, is a widely used tool in biotechnological applications because of its ability to bind biotin strongly. The high p I of avidin (∼10.5), however, is a hindrance in certain applications due to non-specific (charge-related) binding. Here we report a c...