Tandem application of cationic colloidal silica and Triton X-114 for plasma membrane protein isolation and purification: Towards developing an MDCK protein database

• Published in: Proteomics (Weinheim) Vol. 11; no. 7; pp. 1238 - 1253
• Main Authors: Mathias, Rommel A., Chen, Yuan-Shou, Goode, Robert J. A., Kapp, Eugene A., Mathivanan, Suresh, Moritz, Robert L., Zhu, Hong-Jian, Simpson, Richard J.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.04.2011; WILEY‐VCH Verlag; Wiley-VCH; Wiley Subscription Services, Inc
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Blotting, Western; Cationic colloidal silica; Cations - metabolism; Cell adhesion; Cell adhesion & migration; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - isolation & purification; Cell Adhesion Molecules - metabolism; Cell biology; Cell Fractionation; Cell Membrane - chemistry; Cell Membrane - genetics; Cell Membrane - metabolism; Cell surface; Cells, Cultured; Colloids - metabolism; copy number; Data processing; Databases, Protein; Dogs; Drug development; Drugs; Female; Fundamental and applied biological sciences. Psychology; Genomes; hidden Markov models; Hydrophobic and Hydrophilic Interactions; Hydrophobicity; Intercellular signalling; Ion Transport - genetics; Kidney - cytology; Lipids; Mass Spectrometry; MDCK; Membrane proteins; Membrane Proteins - genetics; Membrane Proteins - isolation & purification; Membrane Proteins - metabolism; Miscellaneous; Plasma; Plasma membrane; Plasma membranes; Polyethylene Glycols - metabolism; Protein Array Analysis - methods; protein purification; Protein Structure, Tertiary; Proteins; Proteome - chemistry; Proteome - genetics; Proteome - metabolism; proteomics; Proteomics - methods; Silica; Silicon Dioxide - metabolism; Transmembrane domains; Triton X-114; Membrane protein; Purification; Plasma membrane; Proteomics; Database; Cell biology; Isolation; Cationic colloidal silica; MDCK; Triton X-114

Plasma membrane (PM) proteins are attractive therapeutic targets because of their accessibility to drugs. Although genes encoding PM proteins represent 20–30% of eukaryotic genomes, a detailed characterisation of their encoded proteins is underrepresented, due, to their low copy number and the inher...