Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs : An Application in Pharmaceutical and Human Serum
A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : ...
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| Published in | Journal of analytical methods in chemistry Vol. 2013; no. 2013; pp. 1 - 7 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Cairo, Egypt
Hindawi Puplishing Corporation
01.01.2013
Hindawi Publishing Corporation Wiley |
| Subjects | |
| Online Access | Get full text |
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| Abstract | A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar μBondapak ODS C18 column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation studies proved the stability indicating abilities of the method. |
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| AbstractList | A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar μBondapak ODS C18 column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation studies proved the stability indicating abilities of the method. A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar μ Bondapak ODS C 18 column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation studies proved the stability indicating abilities of the method. A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar μ Bondapak ODS C18 column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation studies proved the stability indicating abilities of the method.A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar μ Bondapak ODS C18 column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation studies proved the stability indicating abilities of the method. A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar mu Bondapak ODS C sub(18) column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation studies proved the stability indicating abilities of the method. A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical parenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of Acetonitrile : buffer : sulfuric acid 0.1 M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar μ Bondapak ODS C18 column monitored at dual wavelength of 266 nm and 205 nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs were subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the molecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear with a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of 98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and trimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify the amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation studies proved the stability indicating abilities of the method. |
| Author | Khan, Sauleha Hasan, Najmul Siddiqui, Muhammad Zain Sher, Nawab Chaiharn, Mathurot Siddiqui, Farhan Ahmed Khalid, Hira |
| AuthorAffiliation | 6 Department of Chemistry, Faculty of Science, Federal Urdu University of Science & Technology, Karachi 75300, Pakistan 2 Division of Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand 5 Faculty of Pharmacy, Federal Urdu University of Science & Technology, Karachi 75300, Pakistan 4 Department of Chemistry, Government College University, Lahore 54000, Pakistan 3 Department of Chemistry, Faculty of Science, University of Karachi, Karachi 75270, Pakistan 1 Department of Microbiology, Faculty of Science, University of Karachi, Karachi 75270, Pakistan |
| AuthorAffiliation_xml | – name: 6 Department of Chemistry, Faculty of Science, Federal Urdu University of Science & Technology, Karachi 75300, Pakistan – name: 4 Department of Chemistry, Government College University, Lahore 54000, Pakistan – name: 1 Department of Microbiology, Faculty of Science, University of Karachi, Karachi 75270, Pakistan – name: 2 Division of Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand – name: 3 Department of Chemistry, Faculty of Science, University of Karachi, Karachi 75270, Pakistan – name: 5 Faculty of Pharmacy, Federal Urdu University of Science & Technology, Karachi 75300, Pakistan |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24286017$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1093_jaoacint_qsac092 crossref_primary_10_2174_1874104501610010033 crossref_primary_10_14500_aro_11375 crossref_primary_10_1016_j_microc_2024_111099 crossref_primary_10_52711_0974_360X_2024_00287 crossref_primary_10_3390_pharmaceutics16101300 crossref_primary_10_4236_ojop_2023_122002 |
| Cites_doi | 10.1016/j.jchromb.2009.11.012 10.1002/bio.740 10.1081/DDC-120003457 10.1016/S1570-0232(03)00316-7 10.1111/j.2042-7158.1961.tb11865.x 10.1016/j.talanta.2007.07.011 10.1016/0039-9140(84)80278-7 10.1053/gast.1996.v110.pm8566580 10.1002/pca.720 10.1016/0378-4347(93)80433-5 10.1016/0003-2697(69)90138-9 10.1021/jf011304n |
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| Copyright | Copyright © 2013 Najmul Hasan et al. Copyright © 2013 Najmul Hasan et al. 2013 |
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| Title | Dual Wavelength RP-HPLC Method for Simultaneous Determination of Two Antispasmodic Drugs : An Application in Pharmaceutical and Human Serum |
| URI | https://search.emarefa.net/detail/BIM-461404 https://dx.doi.org/10.1155/2013/297285 https://www.ncbi.nlm.nih.gov/pubmed/24286017 https://www.proquest.com/docview/1469212879 https://www.proquest.com/docview/1475545997 https://pubmed.ncbi.nlm.nih.gov/PMC3826572 https://doaj.org/article/60c916b44c984b17b05a4b1d04f2f139 |
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